Temporal expression of cytokines and chemokines in rat adjuvant-induced arthritis

Abstract

We discuss the presence of anti-keratin antibodies (AKA) of the IgG class in patients with defined juvenile idiopathic arthritis (JIA). An indirect immunofluorescence test and rat oesophagus substrate was used for the detection and quantification of AKA antibodies in patients´ sera. Overall 33/60 patients with JIA had sera positive for AKA (55 %, P = 0,0001) ranging from 1:10 to 1:160 dilutions. Following idiopathic arthritis of childhood classification criteria AKA occurred in 2/7 patients with systemic disease (28,6 %), in 13/30 patients with RF negative polyarthritis (43,3 %, P = 0,008) and in 15/18 RF positive polyarthritis (83,3 %, P = 0,000002). AKA were also found in a small cohort of patients with oligoarthritis (1/3) and psoriatic arthritis (2/2). AKA positivity occurred in 3/26 healthy controls at a 1:20 dilution. The presence of AKA was correlated as well as with the severity of the disease. Our study revealed that AKA was present overall in 18/29 patients (62%) with severe JIA and in 12/26 patients (46,2 %) with non-severe disease, however this did not reach statistical significance (P = 0,18). We also observed that AKA remained positive regardless of disease activity. AKA were detectable in 55,6 % patients with active JIA and in 48,6 % patients in the complete or near remission. Acknowledgement: This research was supported by a European Commission (Acronym: EUROBANK, contract no: QOL-2000-14.1), web site http://www.ncl.ac.uk and by grant of 2nd Medical Faculty, Charles University in Prague, VZ no. 111300003. P2The significance of antibodies to cyclic citrullinated peptide, antikeratin antibodies, antiperinuclear factor, rheumatoid factor isotypes and HLA shared epitope in prediction of erosive disease in early rheumatoid arthritis patients J Vencovsky, L Sedova, S Machacek, J Gatterova, V Pesakova, J Kafkova and O Krystufkova Institute of Rheumatology, Prague, Czech RepublicObjectives: To evaluate a predictive value of autoantibody examinations in development of erosive disease in early rheumatoid arthritis (RA). Patients and methods: One hundred and fourteen patients with disease duration less than 2 years after the onset of symptoms were investigated. Only patients who fulfilled the diagnostic criteria for RA either at the beginning of the disease or during the follow-up period were included. The antibodies to cyclic citrullinated peptide (anti-CCP) (Immunoscan RA, Euro-diagnostica, The Netherlands), IgM, IgA and IgG rheumatoid factors (RF) were measured by ELISA, antikeratin antibodies (AKA) and antiperinuclear factor (APF) were detected by indirect immunofluorescence, and the presence of HLA shared epitope (HLA SE) was detected by PCR with sequence specific primers. Patients were divided into two groups, either with erosive or non-erosive changes present on the hand or/and feet radiographs at the end of 24 months follow-up. Results: Seventy-six (66.7%) patients developed bony erosion, whereas 38 (33.3%) remained without destructive changes. The initial anti-CCP, AKA, APF, IgM RF, IgA RF, IgG RF ...

Manipulation of the induction of adjuvant arthritis in Sprague-Dawley rats

“…5D). In the study performed by Szekanecz et al, expression of IL-1b in the AIA Lewis rats was found to be similar to TNF-a, while the serum IL-6 levels were increased throughout the time course with abundant production occurring in the late phase of AIA [26]. Actually, these divergent cytokines profi les have been described previously as being due to different animal strains involved and different experimental protocols [33,34].…”

“…Szekanecz et al [26] investigated the correlation between clinical signs and the laboratory analysis along with time-dependent expression of pro-infl ammatory cytokines in female Lewis rats, showing that the production of TNF-a, IL-1b and IL-6 was abundant during the course of AIA induced by subcutaneous injection of 1,500 µg M. butyricum suspended in mineral oil. However, Lussier et al [27] showed that in Lewis rats, 500 µg of M. butyricum could effectively produce the maximal intensity of lesions.…”

Amelioration of Autoimmunity and Inflammation by Zinc Oxide Nanoparticles in Experimental Rheumatoid Arthritis

“…Tumor necrosis factor alpha (TNF-α) and interleukin-1β (IL-1β) are central in ammatory cytokines involved in the pathogenesis of rheumatoid arthritis (RA), since they are involved in bone resorption and cartilage remodeling through maturation of osteoclast cells and inhibition of collagen synthesis (Goldring 2003). In addition, they augment several in ammatory mechanisms such as angiogenesis, brosis and cell adhesion (Szekanecz et al 2000). In the current study ZnO nanoparticles suppressed the serum levels of these cytokines to a level comparable to that of diclofenac Na, the standard drug.…”