Temporal gene expression changes during adipogenesis in human mesenchymal stem cells

Nakamura, Takanori; Shiojima, Satoshi; Hirai, Yuichi; Iwama, Tetsuo; Tsuruzoe, Nobutomo; Hirasawa, Akira; Katsuma, Susumu; Tsujimoto, Gozoh

doi:10.1016/s0006-291x(03)00325-5

Cited by 82 publications

(69 citation statements)

References 27 publications

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“…44 The expression profile during terminal adipogenic differentiation in human MSCs is also similar to that of 3T3-L1 preadipocytes, characterized by early expression of C/EBPb and -d and followed by C/EBPa and PPARg expression. 42 In addition, we have shown specific induction of PPARg2 in human marrow MPCs following addition of adipogenic differentiation media and the levels parallel C/EBPa. 36,37 Therefore, examining the expression of these transcription factors offer insight into paracrine factors regulating adipogenesis in diabetes.…”

Section: Mechanisms Of Enhanced Marrow Adipogenesis In Diabetesmentioning

confidence: 78%

“…The process of differentiation in murine cell lines appears to be similar to the signaling cascade that drives adipogenesis in human bone marrow cells, with the principle actors being peroxisome proliferator-activated receptor g (PPARg) and the CCAAT/enhancer-binding protein (C/EBPa, -b, and -d) transcription factors. 42,43 It should be noted that there are reports of differences between murine cell lines and human MSC/MPCs. For example, Yu and colleagues suggested that human marrow cells primarily express PPARg1 isoform upon differentiation with PPARg2 increases being noted at later time point, which is believed to be in contrast to murine cells.…”

Section: Mechanisms Of Enhanced Marrow Adipogenesis In Diabetesmentioning

confidence: 99%

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Pathophysiological role of enhanced bone marrow adipogenesis in diabetic complications

Piccinin

Khan

2014

Adipocyte

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Section: Mechanisms Of Enhanced Marrow Adipogenesis In Diabetesmentioning

confidence: 78%

Section: Mechanisms Of Enhanced Marrow Adipogenesis In Diabetesmentioning

confidence: 99%

Pathophysiological role of enhanced bone marrow adipogenesis in diabetic complications

Piccinin

Khan

2014

Adipocyte

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“…These precursor cell types allow insights into biochemical pathways leading to adiposity and identification of crucial signaling molecules or pathways that may control the development of obesity and thus the discovery of therapeutic drugs for the disease. [15][16][17] However, invasive surgery is required to harvest bone marrow progenitor cells, causing undue stress and potential risk to the donor. In addition, Stolzing 18 reported that there was a reduced ability to maintain mesenchymal tissue homeostasis in aged mammals as a result of a decline in progenitor cell numbers and functionality due to the accumulation of oxidative damage.…”

Section: Discussionmentioning

confidence: 99%

“…This is consistent with observations made by other groups dealing with mesenchymal cells. 15,17,26 It is expected that there is no PPARg gene expression in naive CLMCs, as this gene is induced only before transcriptional activation of most adipocyte genes. 27 There is no significant difference in the intensity of gene expression among the four differentiation-treatment groups of different glucose and insulin concentrations.…”

Section: Discussionmentioning

confidence: 99%

Cord lining progenitor cells: potential in vitro adipogenesis model

et al. 2010

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Objective: To investigate the effect of glucose and insulin concentrations on differentiation of umbilical cord lining progenitor cells to adipocyte-like cells (ALCs). Methods: Cord lining mesenchymal cells (CLMCs) were isolated from the explant of human umbilical cord amniotic membrane. CLMCs were subjected to differentiation under various culture conditions for 20 days. Lipid droplets were confirmed with Oil Red O staining. Gene expressions of adipsin and peroxisome proliferator-activated receptor gamma (PPARg) were analyzed using reverse transcription-PCR. Leptin and adiponectin secretions were detected using enzyme-linked immunosorbent assay kit. Results: CLMCs became irregular, cuboidal-shaped cells that resemble adipocytes, and Oil Red O staining showed the presence of lipid droplets. The gene expressions of PPARg and adipsin were upregulated. Leptin and adiponectin secretions by naive CLMCs were below the limits of detection. Matured ALCs cultured in low-glucose medium significantly secreted leptin and adiponectin, whereas those in high-glucose medium significantly secreted only leptin. Insulin concentration affects leptin but not adiponectin secretion. Conclusions: Under different culture conditions, CLMCs can differentiate into ALCs that resemble adipocytes in either normalweight or obese individuals. Hence, these ALCs have the potential to be used as an in vitro model to study adipogenesis and obesity, and possibly as a drug discovery model for metabolic disorders.

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“…To induce adipogenic differentiation in vitro, MScs were seeded into a 12-well plate and treated with adipogenic medium (dMeM supplemented with 10% FBS, 0.5 mM 3-isobutyl-1-methylxanthine, 1 µM dexamethasone, 0.2 mM indomethacin and 10 µM insulin) (Sigma) (16), which was changed every 2-3 days until confluence was reached. Adipocytes were identified with Sudan III (Sigma) staining.…”

Section: Methodsmentioning

confidence: 99%

Erythropoietin combined with granulocyte colony‑stimulating factor enhances MMP-2 expression in mesenchymal stem cells and promotes cell migration

Chen

You

et al. 2010

Mol Med Rep

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Abstract. Mesenchymal stem cells (MScs) are promising clinical tools, but the molecular mechanisms that regulate the mobilization and homing of MScs and cause invasion through extracellular matrix (ecM) barriers are unknown. Matrix metalloproteinase (MMP) degrades the ecM and promotes cell migration. in this study, we investigated MMP expression and cell migration after treatment with erythropoietin (ePo) and granulocyte colony-stimulating factor (G-cSF). Specifically, we studied whether EPO combined with G-CSF enhanced MMP expression and increased the in vitro motility of MScs. real-time Pcr was used to detect the mrna of MMPs and tissue inhibitors of metalloproteinase. cell migration was evaluated by transwell and wound healing assays. Western blotting was used to detect changes in erK1/2 protein levels. The results showed that ePo combined with G-cSF enhanced MMP-2 expression in MScs, promoted MSc motility and activated the erK1/2 signaling pathway. Thus, a combination treatment of ePo with G-cSF promoted cell migration by stimulating MMP-2 expression in MScs and this appeared to be related to the erK1/2 signaling pathway.

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Temporal gene expression changes during adipogenesis in human mesenchymal stem cells

Cited by 82 publications

References 27 publications

Pathophysiological role of enhanced bone marrow adipogenesis in diabetic complications

Pathophysiological role of enhanced bone marrow adipogenesis in diabetic complications

Cord lining progenitor cells: potential in vitro adipogenesis model

Erythropoietin combined with granulocyte colony‑stimulating factor enhances MMP-2 expression in mesenchymal stem cells and promotes cell migration

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