Temporal insights into molecular and cellular responses during rAAV production in HEK293T cells

Patra, Alok Tanala; Tan, Evan; Kok, Yee Jiun; Ng, Say Kong; Bi, Xuezhi

doi:10.1016/j.omtm.2024.101278

Molecular Therapy - Methods & Clinical Development

2024

DOI: 10.1016/j.omtm.2024.101278

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Temporal insights into molecular and cellular responses during rAAV production in HEK293T cells

Alok Tanala Patra,

Evan Tan,

Yee Jiun Kok

et al.

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2024

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Cited by 2 publications

References 86 publications

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HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing

Gurazada,

Kennedy,

Braatz

et al. 2024

Biotechnology Advances

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HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing

Gurazada,

Kennedy,

Braatz

et al. 2024

Biotechnology Advances

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Application of the Insect Cell-Baculovirus Expression Vector System in Adeno-Associated Viral Production

Wang,

Sun,

Guo

et al. 2024

Applied Sciences

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Insect Cell-Baculovirus Expression Vector System (IC-BEVS) is an efficient protein expression platform, which is famous for its high-level expression of complex protein in insect cells. The system is based on baculoviruses such as Autographa californica multiple nucleopolyhedrovirus (AcMNPV), and the expression efficiency of the target proteins has been significantly improved by optimizing the viral vectors and cell lines. In recent years, IC-BEVS have shown great potential for Adeno-Associated Virus (AAV) production, particularly excelling in AAV structural protein expression and recombinant AAV production. The system not only improves the yield and purity of AAV, but also shortens the production cycle, providing an efficient and reliable tool for gene therapy. However, the system also has some challenges, including protein modification differences, limitations in expression levels, and production costs. This paper reviews the development of the insect baculovirus expression system, its application in AAV production, and its prospects in gene therapy, aiming to provide a systematic reference and outlook for research in related fields.

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Temporal insights into molecular and cellular responses during rAAV production in HEK293T cells

Cited by 2 publications

References 86 publications

HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing

HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing

Application of the Insect Cell-Baculovirus Expression Vector System in Adeno-Associated Viral Production

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