Temporal origin of mouse claustrum and development of its cortical projections

Hoerder‐Suabedissen, Anna; Ocana-Santero, Gabriel; Draper, Thomas H; Scott, Sophie A.; Kimani, Jesse G.; Shelton, Andrew M; Butt, Simon J. B.; Molnár, Zoltán; Packer, Adam M.

doi:10.1093/cercor/bhac318

Cited by 12 publications

(26 citation statements)

References 72 publications

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“…We performed unilateral injections of retro-AAV-CAG-GFP into the RSC of adult mice (>P70) and analyzed GFP expression in the claustrum 14 days post injection ( Figure 1A-C ). Several studies found Nurr1 to be a relatively selective marker for the claustrum at different ages and across different species, including rodents and primates (10,11,13,20). We found that Nurr1 immunolabelling showed a dense population of cells that appeared to be spatially aligned with the GFP-labeled retro-RSC central zone ( Figure 1C, D ).…”

Section: Resultsmentioning

confidence: 99%

A simple and reliable method for claustrum localization across age in mice

Shaker,

Dagpa,

Cattaud

et al. 2023

Preprint

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1.ABSTRACTThe anatomical organization of the rodent claustrum remains obscure due to lack of clear borders that distinguish it from neighboring forebrain structures. Defining what constitutes the claustrum is imperative for elucidating its functions. Methods based on gene/protein expression or transgenic mice have been used to spatially outline the claustrum but often report incomplete labeling and/or lack of specificity during certain neurodevelopmental timepoints. To reliably identify claustrum cells in mice, we propose a simple immunolabelling method that juxtaposes the expression pattern of claustrum-enriched and cortical-enriched markers. We determined that claustrum cells immunoreactive for the claustrum-enriched markers Nurr1 and Nr2f2 are devoid of the cortical marker Tle4, which allowed us to differentiate the claustrum from adjoining cortical cells. Using retrograde tracing, we verified that nearly all claustrum projection neurons lack Tle4 but expressed Nurr1/Nr2f2 markers to different degrees. At neonatal stages between 7 and 21 days, claustrum projection neurons were identified by their Nurr1-postive/Tle4-negative expression profile, a time-period when techniques used to localize the claustrum in adult mice are ineffective. Finally, exposure to environmental novelty enhanced the expression of the neuronal activation marker cFos in the claustrum region. Notably, cFos labeling was mainly restricted to Nurr1-positive cells and nearly absent from Tle4-positive cells, thus corroborating previous work reporting novelty-induced claustrum activation. Taken together, this method will aid in studying the claustrum during postnatal development and may improve histological and functional studies where other approaches are not amenable.

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Section: Resultsmentioning

confidence: 99%

A simple and reliable method for claustrum localization across age in mice

Shaker,

Dagpa,

Cattaud

et al. 2023

Preprint

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“…Similar experiments performed on rats observed that claustral populations are produced between E11.5 and E14.5 with a peak at E12.5 (22). The most recent study by Hoerder-Suabedissen (34) on neurogenesis of the claustrum in mice indicated that claustrum neurons were born at a short time window, E12.5 and neurons in endopiriform nucleus were born at E11.5. Here we found that claustrum neurons in macaques are generated later than subplate neurons of the insular cortex.…”

Section: Discussionmentioning

confidence: 99%

Origin and Development of the Claustrum in Rhesus Macaque

Duque

Rakić

2023

Preprint

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Understanding the claustrum functions has recently progressed thanks to new anatomical and behavioral studies in rodents, which suggest that it plays an important role in attention, salience detection, slow-wave generation, and neocortical network synchronization. Nevertheless, knowledge about the origin and development of the claustrum, especially in primates, is still limited. Here, we show that neurons of rhesus macaque claustrum primordium are generated between embryonic day E48 and E55 and express some neocortical molecular markers, such as NR4A2, SATB2, and SOX5. However, in the early stages, it lacks TBR1 expression, which separates it from other surrounding telencephalic structures. We also found that two waves of neurogenesis (E48 and E55) in the claustrum, corresponding to the birthdates of layers 6 and 5 of the insular cortex, establish a core and shell cytoarchitecture, which is potentially a basis for differential circuit formation and could influence information processing underlying higher cognitive functions of the claustrum. In addition, parvalbumin-positive interneurons are the dominant interneuron type in the claustrum in fetal macaque, and their maturation is independent of that in the overlaying neocortex. Finally, our study reveals that the claustrum is likely not a continuance of subplate neurons of the insular cortex, but an independent pallial region, suggesting its potentially unique role in cognitive control.

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“…Intracerebral delivery of viral vectors was performed as previously described . In brief, mice were anaesthetised with isoflurane, head-fixed in a stereotaxic frame and a 1 cm incision was performed on the scalp prior to craniotomy (coordinates AP = −1.9, ML = 3.0 from bregma).…”

Section: Methodsmentioning

confidence: 99%

In Vivo Two-Photon Microscopy Reveals Sensory-Evoked Serotonin (5-HT) Release in Adult Mammalian Neocortex

Ocana-Santero,

Packer,

Sharp

et al. 2024

ACS Chem. Neurosci.

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The recent development of genetically encoded fluorescent neurotransmitter biosensors has opened the door to recording serotonin (5-hydroxytryptamine, 5-HT) signaling dynamics with high temporal and spatial resolution in vivo. While this represents a significant step forward for serotonin research, the utility of available 5-HT biosensors remains to be fully established under diverse in vivo conditions. Here, we used two-photon microscopy in awake mice to examine the effectiveness of specific 5-HT biosensors for monitoring 5-HT dynamics in somatosensory cortex. Initial experiments found that whisker stimulation evoked a striking change in 5-HT biosensor signal. However, similar changes were observed in controls expressing green fluorescent protein, suggesting a potential hemodynamic artifact. Subsequent use of a second control fluorophore with emission peaks separated from the 5-HT biosensor revealed a reproducible, stimulus-locked increase in 5-HT signal. Our data highlight the promise of 5-HT biosensors for in vivo application, provided measurements are carried out with appropriate optical controls.

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Temporal origin of mouse claustrum and development of its cortical projections

Cited by 12 publications

References 72 publications

A simple and reliable method for claustrum localization across age in mice

A simple and reliable method for claustrum localization across age in mice

Origin and Development of the Claustrum in Rhesus Macaque

In Vivo Two-Photon Microscopy Reveals Sensory-Evoked Serotonin (5-HT) Release in Adult Mammalian Neocortex

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