Temporal pattern and effect of sex on lipopolysaccharide-induced stress hormone and cytokine response in pigs

Williams, Phillip N.; Collier, C.T.; Carroll, J.A.; Welsh, T. H.; Laurenz, Jamie C.

doi:10.1016/j.domaniend.2009.04.004

Cited by 53 publications

(47 citation statements)

References 26 publications

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“…In some other studies, maximal IL-1␤ levels were only seen at 3 h p.a. (Williams et al, 2009;Wyns et al, 2013). Interestingly, the studies describing a noticeable increase or maximal concentration of IL-1␤ p.a., all applied E. coli serotype O111:B4 (Table 1).…”

Section: Pro-inflammatory Cytokinesmentioning

confidence: 92%

“…Noteworthy, in setups utilizing frequent blood sampling, maximal levels of IL-6 were repetitively established at 2.5 h p.a. (Frank et al, 2003;Carroll et al, 2005;Williams et al, 2009;Wyns et al, 2013). Although IL-6 is a potent inducer of the APR, it has anti-inflammatory properties as well.…”

Section: Pro-inflammatory Cytokinesmentioning

confidence: 99%

“…Williams et al (2009) determined an increase in SAA, CRP as well as Hp after an i.v. LPS bolus injection of 25 g/kg BW.…”

Section: Porcine Acute Phase Proteinsmentioning

confidence: 99%

“…Different LPS serotypes and preparations are commercially available of which O111:B4 is indisputably the most frequently used in porcine research (Frank et al, 2003;Carroll et al, 2005;Williams et al, 2009). However, protein impurities in those LPS preparations influence the transcriptional profile as both TLR2-and TLR4-mediated signaling can occur (Hirschfeld et al, 2000;Lorne et al, 2010;Rutledge et al, 2012).…”

Section: Lps Challengementioning

confidence: 99%

See 3 more Smart Citations

In vivo porcine lipopolysaccharide inflammation models to study immunomodulation of drugs

Wyns

Plessers

Backer

et al. 2015

Veterinary Immunology and Immunopathology

103

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Section: Pro-inflammatory Cytokinesmentioning

confidence: 92%

Section: Pro-inflammatory Cytokinesmentioning

confidence: 99%

“…Williams et al (2009) determined an increase in SAA, CRP as well as Hp after an i.v. LPS bolus injection of 25 g/kg BW.…”

Section: Porcine Acute Phase Proteinsmentioning

confidence: 99%

Section: Lps Challengementioning

confidence: 99%

See 2 more Smart Citations

In vivo porcine lipopolysaccharide inflammation models to study immunomodulation of drugs

Wyns

Plessers

Backer

et al. 2015

Veterinary Immunology and Immunopathology

103

View full text Add to dashboard Cite

“…LPS activates the innate immune response and induces the release of pro-inflammatory cytokines in pigs (Williams et al 2009). LPS and inflammatory cytokines activate NF-kB, a family of transcription factors central to activation of the immune response (Oeckinghaus et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Septic shock non-thyroidal illness syndrome causes hypothyroidism and conditions for reduced sensitivity to thyroid hormone

Castro¹,

Quisenberry²,

Calvo³

et al. 2013

Journal of Molecular Endocrinology

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Non-thyroidal illness syndrome (NTIS) is part of the neuroendocrine response to stress, but the significance of this syndrome remains uncertain. The aim of this study was to investigate the effect of lipopolysaccharide (LPS)-induced NTIS on thyroid hormone (TH) levels and TH molecular targets, as well as the relationship between septic shock nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) activation and TH receptor b (THRB) gene expression at a multi-tissue level in a pig model. Prepubertal domestic pigs were given i.v. saline or LPS for 48 h. Serum and tissue TH was measured by chemiluminescence and RIA. Expression of THRs and cofactors was measured by real-time PCR, and deiodinase (DIO) activity was measured by enzyme assays. Tissue NF-kB nuclear binding activity was evaluated by EMSA. LPS-treated pigs had decreased TH levels in serum and most tissues. DIO1 expression in liver and kidney and DIO1 activity in kidney decreased after LPS. No changes in DIO2 activity were observed between groups. LPS induced an increase in hypothalamus, thyroid, and liver DIO3 activity. Among the other studied genes, monocarboxylate transporter 8 and THRB were the most commonly repressed in endotoxemic pigs. LPS-induced NF-kB activation was associated with a decrease in THRB gene expression only in frontal lobe, adrenal gland, and kidney cortex. We conclude that LPS-induced NTIS in pigs is characterized by hypothyroidism and tissue-specific reduced TH sensitivity. The role of NF-kB in regulating THRB expression during endotoxemia, if any, is restricted to a limited number of tissues.

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Peripheral tumor necrosis factor α regulation of adipose tissue metabolism and adipokine gene expression in neonatal pigs

Ramsay

Stoll²,

Conde-Aguilera³

et al. 2012

Vet Res Commun

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The neonatal pig is susceptible to stress and infection, conditions which favor tumor necrosis factor α (TNFα) secretion. This study examined whether TNFα can alter metabolic activity and cytokine gene expression within neonatal pig adipose tissue. Cell cultures were prepared from neonatal subcutaneous adipose tissue using standard procedures. Cultures (5 experiments) were incubated with medium containing (14)C-glucose for 4 h to measure glucose conversion to lipid in the presence of combinations of TNFα (10 ng), insulin (10 nM) and an anti-pig TNFα antibody (5 μg). Basal lipogenesis was not affected by TNFα treatment (P > 0.05). However, insulin stimulated lipogenesis was reduced by TNFα (P < 0.02). For gene expression studies, cultures were incubated with 0, 2.5, 5.0 or 10 ng TNFα for 2, 4 or 24 h (n = 4 experiments). Interleukin 6 and TNFα gene expression were acutely (2-4 h) stimulated by exogenous TNFα treatment (P < 0.05), as analyzed by real-time PCR. Adiponectin mRNA abundance was reduced (P < 0.001) while monocyte chemotactic gene expression was increased by TNFα treatment at all time points (P < 0.001). Chronic treatment (24 h) was required to increase monocyte multiplication inhibitory factor or suppress lipoprotein lipase gene expression (P < 0.02). These data suggest conditions which increase serum TNFα, like sepsis, could suppress lipid accumulation within adipose tissue at a time of critical need in the neonate and induce a variety of adipose derived cytokines which may function to alter adipose physiology.

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Temporal pattern and effect of sex on lipopolysaccharide-induced stress hormone and cytokine response in pigs

Cited by 53 publications

References 26 publications

In vivo porcine lipopolysaccharide inflammation models to study immunomodulation of drugs

In vivo porcine lipopolysaccharide inflammation models to study immunomodulation of drugs

Septic shock non-thyroidal illness syndrome causes hypothyroidism and conditions for reduced sensitivity to thyroid hormone

Peripheral tumor necrosis factor α regulation of adipose tissue metabolism and adipokine gene expression in neonatal pigs

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