Temporal patterns of alterations in obesity index, lipid profile, renal function and blood pressure during the development of hypertension in male, but not female, rats fed a moderately high-fat diet

Nazari, Somayeh; Moosavi, Seyed Mostafa Shid

doi:10.1080/13813455.2020.1739713

Cited by 2 publications

(7 citation statements)

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“…After a 15 min acclimatization period, the average of the three most consistent of five systolic blood pressure (P S ) measurements was recorded using a PowerLab/4SP data acquisition system (ADInstruments). The animal remained in the restrainer covered by a towel and its tail was warmed to facilitate collection of a 2 ml blood sample from the lateral tail vein (Nazari & Moosavi, 2020).…”

Section: Animals and Experimental Groupsmentioning

confidence: 99%

“…After the collection of the basal data on day 0, 12 rats were randomly selected to remain on the LF diet (LF group) and the remainder were switched onto a MHF diet (32% kcal as fat), which was prepared as described previously (Nazari & Moosavi, 2020). nasoanal length, OI and P S were measured weekly, while metabolic cage studies and tail-blood sampling were performed every other week (even weeks).…”

Section: Animals and Experimental Groupsmentioning

confidence: 99%

“…The renal excretory response to acute salt loading was evaluated as previously described (Nazari & Moosavi, 2020). On the day after the 10-week dietary period, rats were injected with normal saline (I.P.,7.5% BW) over 15 min and immediately placed in individual metabolic cages.…”

Section: Acute Salt Loading Experimentsmentioning

confidence: 99%

“…Thereafter, a gentle separation of the left renal artery and vein was performed using the surgical microscope to allow placement of a perivascular probe (MA1PRB) of a Transonic flowmeter (T402-PB; Transonic Systems Inc., Ithaca, NY, USA) around the left renal artery and connected to the PowerLab for measurement of renal blood flow (RBF) (Nazari & Moosavi, 2020). The rat was allowed to equilibrate for 30 min before the start of the experimental protocol.…”

Section: Renal Autoregulatory Experimentsmentioning

confidence: 99%

“…Renal vascular resistance (RVR) at each clearance period was also calculated by dividing the average RPP by the average RBF corrected for kidney weight. The volume of urine samples at each clearance period was measured and all urine and plasma samples were stored for later assessment of their creatinine and sodium concentrations (Nazari & Moosavi, 2020). After killing the rat with an overdose of pentobarbitone anaesthetic, both kidneys and intra-abdominal fat depots including retroperitoneal, mesenteric, perirenal and epididymal fat pads were carefully removed and separately weighed.…”

Section: Renal Autoregulatory Experimentsmentioning

confidence: 99%

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Bilateral renal denervation prevents the development of hypertension during diet‐induced obesity in male rats

Nazari

Haghani

Moosavi

2021

Experimental Physiology

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Feeding a moderately high-fat (MHF) diet in male Sprague-Dawley rats induces obesity, pressure natriuresis impairment and hypertension. This study investigated the role of the renal nerves in the impaired pressure natriuresis and hypertension caused by feeding a MHF diet. After collecting baseline data on day 0, 12 rats remained on a low-fat diet (LF group) while the others were switched onto a MHF diet and diverged into obesity-resistant (OR) or obesity-prone (OP). After 4 weeks, half of the OR and OP rats underwent bilateral renal denervation (BRD) to generate four groups: OR, OR/BRD, OP and OP/BRD (n = 12). During 10 weeks, body weight, obesity index, systolic pressure and renal excretory function were measured regularly. After 10 weeks, renal excretory responses to acute salt loading and renal autoregulation were evaluated. The OP and OP/BRD groups had greater increases of body weight and obesity index during the dietary period compared to the other groups, and by week 10 their body weight (425.1 ± 7.2 and 411.9 ± 5.1 g) became considerably larger than that of the LF group (358.5 ± 6.2 g). Renal sodium excretion was reduced by ∼20% at week 4 in the OP and OP/BRD groups, while only the OP group had lower sodium excretion at weeks 6-8 and higher systolic pressure over weeks 5-10 than the other groups and its week 10 systolic pressure reached 138.1 ± 6.7 versus 123.6 ± 2.7 mmHg of the LF group. The OP group showed delayed renal excretory responses to salt loading with rightward and downward shifts in renal autoregulatory curves. Therefore, the renal nerves exert a main mediatory role in the development of pressure natriuresis impairment and hypertension as obesity is established due to the long-term consumption of the MHF diet in male OP rats.

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Section: Animals and Experimental Groupsmentioning

confidence: 99%

Section: Animals and Experimental Groupsmentioning

confidence: 99%

Section: Acute Salt Loading Experimentsmentioning

confidence: 99%

Section: Renal Autoregulatory Experimentsmentioning

confidence: 99%

Section: Renal Autoregulatory Experimentsmentioning

confidence: 99%

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