Temporal patterns of gene expression of G1-S cyclins and cdks during the first and second mitotic cell cycles in mouse embryos

Moore, Grace D.; Ayabe, Takuya; Kopf, Gregory S.; Schultz, Richard M.

doi:10.1002/(sici)1098-2795(199611)45:3<264::aid-mrd2>3.0.co;2-q

Cited by 71 publications

(49 citation statements)

References 40 publications

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“…2C). However, injection of the miR-34c inhibitor 30 h after hCG injection, when DNA synthesis of the zygotes had been completed (15), did not affect the first cell division; more than 95% of the injected zygotes in both groups formed two-cell embryos.…”

Section: Resultsmentioning

confidence: 99%

Sperm-borne microRNA-34c is required for the first cleavage division in mouse

Liu

Pang

Chiu

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

386

335

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In mammals, the sperm deliver mRNA of unknown function into the oocytes during fertilization. The role of sperm microRNAs (miRNAs) in preimplantation development is unknown. miRNA profiling identified six miRNAs expressed in the sperm and the zygotes but not in the oocytes or preimplantation embryos. Sperm contained both the precursor and the mature form of one of these miRNAs, miR-34c. The absence of an increased level of miR-34c in zygotes derived from α-amanitin-treated oocytes and in parthenogenetic oocytes supported a sperm origin of zygotic miR-34c. Injection of miR-34c inhibitor into zygotes inhibited DNA synthesis and significantly suppressed first cleavage division. A 3′ UTR luciferase assay and Western blotting demonstrated that miR-34c regulates B-cell leukemia/lymphoma 2 (Bcl-2) expression in the zygotes. Coinjection of anti-Bcl-2 antibody in zygotes partially reversed but injection of Bcl-2 protein mimicked the effect of miR-34c inhibition. Oocyte activation is essential for the miR-34c action in zygotes, as demonstrated by a decrease in 3′ UTR luciferase reporter activity and Bcl-2 expression after injection of precursor miR-34c into parthenogenetic oocytes. Our findings provide evidence that sperm-borne miR-34c is important for the first cell division via modulation of Bcl-2 expression.

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Section: Resultsmentioning

confidence: 99%

Sperm-borne microRNA-34c is required for the first cleavage division in mouse

Liu

Pang

Chiu

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

386

335

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“…All the three D-type cyclins were shown to be present in unfertilized mouse oocytes (Moore et al, 1996;Kohoutek et al, 2004); cyclin D3 levels decrease sharply during the first cell cycle (Kohoutek et al, 2004). Similarly, the levels of the catalytic partners for D-type cyclins, CDK4 and CDK6 rapidly decrease at the beginning of mouse embryo development (Kohoutek et al, 2004).…”

Section: D-cyclins Cdk4 and Cdk6mentioning

confidence: 98%

“…Similarly, the levels of the catalytic partners for D-type cyclins, CDK4 and CDK6 rapidly decrease at the beginning of mouse embryo development (Kohoutek et al, 2004). Moreover, the major target of cyclin D-CDK4/6 complexes -the retinoblastoma protein-is drastically downregulated at the two-cell stage and remains absent until the late blastocyst stage (Moore et al, 1996;Iwamori et al, 2002). The absence of pRb in early embryonic cells would be predicted to result in a constitutive activation of E2F transcription factors, even without cyclin D/CDK4/6 activity.…”

Section: D-cyclins Cdk4 and Cdk6mentioning

confidence: 99%

“…However, several important observations about the core cell cycle machinery during these early embryonic cycles have been reported. Thus, the majority of proteins deemed responsible for the regulation of G1, S, G2 and M phases are present in preimplantation mouse embryos (Choi et al, 1991;Moore et al, 1996;Palena et al, 2000;Kohoutek et al, 2004). However, some observations questioned the importance of certain cell cycle regulators during the early cell cycles.…”

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Cell cycle in mouse development

2005

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Mice likely represent the most-studied mammalian organism, except for humans. Genetic engineering in embryonic stem cells has allowed derivation of mouse strains lacking particular cell cycle proteins. Analyses of these mutant mice, and cells derived from them, facilitated the studies of the functions of cell cycle apparatus at the organismal and cellular levels. In this review, we give some background about the cell cycle progression during mouse development. We next discuss some insights about in vivo functions of the cell cycle proteins, gleaned from mouse knockout experiments. Our text is meant to provide examples of the recent experiments, rather than to supply an extensive and complete list.

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“…Among others, the role of cyclins Cdk2 and Cdk4 was analysed in the cell cycle progress during meiotic and mitotic divisions in mouse embryos (Moore et al, 1996). It was found that the abundance of Cdk2 and Cdk4 was increased during the S and/or G2 phases of the first mitotic division.…”

Section: A B C Dmentioning

confidence: 99%

Expression and cellular distribution of cyclin-dependent kinase 4 (Cdk4) and connexin 43 (Cx43) in porcine oocytes before and after in vitro maturation

Kempisty

Ziółkowska

Piotrowska

et al. 2014

Acta Veterinaria Hungarica

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It is recognised that connexin 43 (Cx43) and cyclin-dependent kinase 4 (Cdk4) are involved in the cumulus cell-oocyte communication via gap junctions and the control of cell cycle progress. However, little is known about their mRNA expression pattern and encoded proteins distribution in porcine oocytes during in vitro maturation (IVM). Cumulus-oocyte complexes (COCs) were collected from 31 puberal crossbred Landrace gilts and analysed for their Cdk4 and Cx43 mRNA expression using RQ-PCR and for the respective protein expression by confocal microscopic observations. An increased Cdk4 and Cx43 mRNA expression was found in oocytes after IVM (P < 0.001 and P < 0.05, respectively). Confocal microscopic observations revealed a significant increase of Cdk4 protein expression in the cytoplasm of oocytes during the maturation process. The localisation of Cx43 changed from zona pellucida before to cytoplasm of oocytes after IVM. It is supposed that the increased expression of Cdk4 and Cx43 mRNA in oocytes after IVM is linked with the accumulation of a large amount of templates during the process of oocyte maturation. The translocation especially of Cx43 from the zona pellucida into the cytoplasm may be associated with a decrease in gap junction activity in fully grown porcine oocytes. Both Cdk4 and Cx43 can be used as 'checkpoints' of oocyte maturation.

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Temporal patterns of gene expression of G1-S cyclins and cdks during the first and second mitotic cell cycles in mouse embryos

Cited by 71 publications

References 40 publications

Sperm-borne microRNA-34c is required for the first cleavage division in mouse

Sperm-borne microRNA-34c is required for the first cleavage division in mouse

Cell cycle in mouse development

Expression and cellular distribution of cyclin-dependent kinase 4 (Cdk4) and connexin 43 (Cx43) in porcine oocytes before and after in vitro maturation

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