Temporal regulation of cell-wall pectin methylesterase and peroxidase isoforms in cadmium-treated flax hypocotyl

Paynel, Florence; Schaumann, Annick; Arkoun, Mustapha; Douchiche, Olfa; Morvan, C.

doi:10.1093/aob/mcp254

Cited by 58 publications

(36 citation statements)

References 60 publications

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“…In addition to increase polysaccharides levels in roots, Cd also notably increased PME activity in roots. This phenomenon was also observed in several previous studies (Astier et al 2014;Paynel et al 2009;Zhu et al 2012). One possible explanation was that Cd may increase ethylene production in roots (Masood et al 2012;Schellingen et al 2014), which is known to up-regulate PME gene expression and activity (Chervin et al 2008;Wei et al 2010), thus increasing the PME activity in roots.…”

Section: Discussionsupporting

confidence: 85%

Cadmium impairs iron homeostasis in Arabidopsis thaliana by increasing the polysaccharide contents and the iron-binding capacity of root cell walls

Lin

Lai

2015

Plant Soil

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Background and aims Iron (Fe) deficiency is a wellknown symptom of cadmium (Cd) toxicity. Here, the mechanisms underlying Cd impairment of Fe homeostasis in Arabidopsis were investigated. Methods Arabidopsis plants were subjected to 0 (CK) or 10 μM Cd treatments. After the treatments period, Fe concentrations, expression levels of Fe uptake-and translocation-related genes, polysaccharide concentrations, pectin methylesterase activities (PME) and the degree of pectin methylation (DM) were analyzed.

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Section: Discussionsupporting

confidence: 85%

Cadmium impairs iron homeostasis in Arabidopsis thaliana by increasing the polysaccharide contents and the iron-binding capacity of root cell walls

Lin

Lai

2015

Plant Soil

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“…Semi‐quantitative RT‐PCR of the corresponding LuPME5 gene in developing flax hypocotyls suggested that the gene might play a role in wall stiffening. More recently, a flax PME has been implicated in wall modifications following cadmium treatment . We also identified a pectinesterase inhibitor and a polygalacturonase inhibiting protein.…”

Section: Discussionmentioning

confidence: 86%

Identification of cell wall proteins in the flax (Linum usitatissimum ) stem

et al. 2013

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Sequential salt (CaCl2 , LiCl) extractions were used to obtain fractions enriched in cell wall proteins (CWPs) from the stem of 60-day-old flax (Linum usitatissimum) plants. High-resolution FT-ICR MS analysis and the use of recently published genomic data allowed the identification of 11 912 peptides corresponding to a total of 1418 different proteins. Subcellular localization using TargetP, Predotar, and WoLF PSORT led to the identification of 152 putative flax CWPs that were classified into nine different functional classes previously established for Arabidopsis thaliana. Examination of different functional classes revealed the presence of a number of proteins known to be involved in, or potentially involved in cell-wall metabolism in plants. The flax stem cell wall proteome was also compared with transcriptomic data previously obtained on comparable samples. This study represents a major contribution to the identification of CWPs in flax and will lead to a better understanding of cell wall biology in this species.

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“…Here, the amount of pectin with non-blockwise pattern of de-esterification (LM7 epitope) significantly increased, compensating for the marked decrease of JIM7 epitope, while JIM5 epitope remained almost constant and the 2F4 epitope shifted down. These data again pointed to the possible role of a pectin methylesterase under Cd stress (Paynel et al 2009) and concluded that the increase of LM7 labeling in the external tangential wall of the epidermis, together with a decrease of JIM7 labeling, suggested a specific role for randomly partially de-esterified pectins in counteracting the radial swelling stress (Douchiche et al 2010).…”

Section: Detection Of Tms In Plant Cell Wallmentioning

confidence: 82%

“…Further studies concerning L. usitatissimum indicated that Cd also led to an increase in pectin methylesterase (PME) activity (Paynel et al 2009). PME, as described above, is the enzyme responsible for the demethylation of high-methylesterified pectins and the formation of low-mehylesterified pectin epitopes (Willats et al 2006;Pelloux et al 2007).…”

Section: Detection Of Tms In Plant Cell Wallmentioning

confidence: 99%

The cell wall in plant cell response to trace metals: polysaccharide remodeling and its role in defense strategy

2010

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This review paper is focused predominantly on the role of the cell wall in the defense response of plants to trace metals. It is generally known that this compartment accumulates toxic divalent and trivalent metal cations both during their uptake by the cell from the environment and at the final stage of their sequestration from the protoplast. However, from results obtained in recent years, our understanding of the role played by the cell wall in plant defense response to toxic metals has markedly altered. It has been shown that this compartment may function not only as a sink for toxic trace metal accumulation, but that it is also actively modified under trace metal stress. These modifications lead to an increase in the capacity of the cell wall to accumulate trace metals and a decrease of its permeability for trace metal migration into the protoplast. One of the most striking alterations is the enhancement of the level of low-methylesterified pectins: the polysaccharides able to bind divalent and trivalent metal ions. This review paper will present the most recent results, especially those that are concerned with polysaccharide level, composition and distribution under trace metal stress, and describe in detail the polysaccharides responsible for metal binding and immobilization in different groups of plants (algae and higher plants). The review also contains information related to the entry pathways of trace metals into the cell wall and their detection methods.

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Temporal regulation of cell-wall pectin methylesterase and peroxidase isoforms in cadmium-treated flax hypocotyl

Cited by 58 publications

References 60 publications

Cadmium impairs iron homeostasis in Arabidopsis thaliana by increasing the polysaccharide contents and the iron-binding capacity of root cell walls

Cadmium impairs iron homeostasis in Arabidopsis thaliana by increasing the polysaccharide contents and the iron-binding capacity of root cell walls

Identification of cell wall proteins in the flax (Linum usitatissimum ) stem

The cell wall in plant cell response to trace metals: polysaccharide remodeling and its role in defense strategy

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