Temporal variation of Skeletonema community composition from a long-term time series in Narragansett Bay identified using high-throughput DNA sequencing

Canesi, Kelly; Rynearson, Tatiana A.

doi:10.3354/meps11843

Cited by 50 publications

(76 citation statements)

References 57 publications

(72 reference statements)

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“…Based on field observations, it has been proposed that genetic and phenotypic variation among species in the genus Skeletonema influence their temporal and spatial distributions (Kooistra et al. , Canesi and Rynearson ). In the dynamic coastal and estuarine ecosystems that Skeletonema inhabits, there may be a trade‐off among species as environmental conditions shift between habitats or throughout an annual cycle.…”

Section: Discussionmentioning

confidence: 99%

“…In the dynamic coastal and estuarine ecosystems that Skeletonema inhabits, there may be a trade‐off among species as environmental conditions shift between habitats or throughout an annual cycle. For example, in Narragansett Bay, six Skeletonema species comprise and often numerically dominate different blooms over the course of a year when dissolved inorganic nitrogen concentrations range from 0.14 to 18.94 μM (Canesi and Rynearson ). Environmentally variable ecosystems inhabited by multiple Skeletonema species present an opportunity to examine Skeletonema gene expression in situ to further examine the relationship between nitrogen conditions and nitrogen uptake, to define the physiological characteristics among species and to better understand the process of species succession.…”

Section: Discussionmentioning

confidence: 99%

“…There appear to be differences in geographic distributions among species (Kooistra et al. ) as well as temporal species succession within individual locations (Canesi and Rynearson ) suggesting that there are significant ecological differences among these closely related species. Variation in nutrient metabolism among Skeletonema species may help to explain species succession and geographic distributions in the ocean.…”

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confidence: 99%

“…These species were chosen because they have different seasonal cycles, with S. marinoi and S. dohrnii being most common during winter and spring and S. menzelii , during summer (Kaeriyama et al. , Canesi and Rynearson ).…”

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confidence: 99%

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Identification and Expression Analyses of the Nitrate Transporter Gene (NRT2) Family Among Skeletonema species (Bacillariophyceae)

Kang

Rynearson

2019

Journal of Phycology

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High‐affinity nitrate transporters are considered to be the major transporter system for nitrate uptake in diatoms. In the diatom genus Skeletonema, three forms of genes encoding high‐affinity nitrate transporters (NRT2) were newly identified from transcriptomes generated as part of the marine microbial eukaryote transcriptome sequencing project. To examine the expression of each form of NRT2 under different nitrogen environments, laboratory experiments were conducted under nitrate‐sufficient, ammonium‐sufficient, and nitrate‐limited conditions using three ecologically important Skeletonema species: S. dohrnii, S. menzelii, and S. marinoi. Primers were developed for each NRT2 form and species and Q‐RT‐PCR was performed. For each NRT2 form, the three Skeletonema species had similar transcriptional patterns. The transcript levels of NRT2:1 were significantly elevated under nitrogen‐limited conditions, but strongly repressed in the presence of ammonium. The transcript levels of NRT2:2 were also repressed by ammonium, but increased 5‐ to 10‐fold under nitrate‐sufficient and nitrogen‐limited conditions. Finally, the transcript levels of NRT2:3 did not vary significantly under various nitrogen conditions, and behaved more like a constitutively expressed gene. Based on the observed transcript variation among NRT2 forms, we propose a revised model describing nitrate uptake kinetics regulated by multiple forms of nitrate transporter genes in response to various nitrogen conditions in Skeletonema. The differential NRT2 transcriptional responses among species suggest that species‐specific adaptive strategies exist within this genus to cope with environmental changes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Identification and Expression Analyses of the Nitrate Transporter Gene (NRT2) Family Among Skeletonema species (Bacillariophyceae)

Kang

Rynearson

2019

Journal of Phycology

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“…Among them, the genus Skeletonema is distributed ubiquitously in coastal environments, and often predominates numerically in embayments, such as Tokyo Bay (Yoshida et al. ), Narragansett Bay (Canesi and Rynearson ), Chesapeake Bay (Marshall ), and the Gulf of Naples (Ribera d'Alcalà et al. ).…”

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confidence: 99%

Effect of cell size on growth rates of two Skeletonema species and their competitve interactions

Ando

Katano

2018

Journal of Phycology

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We investigated shifts in growth rates and cell size reduction-restoration processes in two species of diatoms, Skeletonema japonicum and Skeletonema dohrnii. Growth rates greatly fluctuated from 1.0 to 2.1 per day, even under the same conditions. Rates increased from 1.1 to 2.1 per day with decreasing valve diameter; however, rates quickly decreased to 1.0 per day when cell size reached the threshold for initiating auxosporulation. We also conducted co-culture experiments using different size combinations of the two species. The experiment was started using the same cell densities (75 cells · mL ) of both species, and when batch cultures reached late exponential phase, the cultures were reinoculated twice into new medium. When large (valve diameter of 17 μm) S. japonicum cells and small (6 μm) S. dohrnii cells were co-cultured, the S. dohrnii contributed 99% of the total cell density on day 16 (S. dohrnii: 263,900 cells · mL ; S. japonicum: 2,000 cells · mL ). In contrast, when small (9 μm) S. japonicum cells and large (15 μm) S. dohrnii cells were co-cultured, small S. japonicum cells accounted for 97% of the total cell density after only 13 d (S. dohrnii: 1,900 cells · mL ; S. japonicum: 62,500 cells · mL ). This study demonstrated that diatom growth rates covary with cell size, and this phenomenon potentially determines the outcome of competition between species.

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Recent Insights into Diatom Distributions and the Contributions of Molecular Approaches

Kulichová,

Trobajo

2024

Diatom Ecology

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Temporal variation of Skeletonema community composition from a long-term time series in Narragansett Bay identified using high-throughput DNA sequencing

Cited by 50 publications

References 57 publications

Identification and Expression Analyses of the Nitrate Transporter Gene (NRT2) Family Among Skeletonema species (Bacillariophyceae)

Identification and Expression Analyses of the Nitrate Transporter Gene (NRT2) Family Among Skeletonema species (Bacillariophyceae)

Effect of cell size on growth rates of two Skeletonema species and their competitve interactions

Recent Insights into Diatom Distributions and the Contributions of Molecular Approaches

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