Temporal variations of glandular kallikrein, protein and amylase in mixed human saliva

Jenzano, Joyce W.; Brown, C.K.; Mauriello, Sally M.

doi:10.1016/0003-9969(87)90123-3

Cited by 38 publications

(25 citation statements)

References 14 publications

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“…The diurnal variation of SAA showed a lower value in the morning and then increased during the daytime. This result well agreed with previous reports [13,14]. Since SAA w showed lower levels than SAA h at all times, sympathetic nervous activity on the workday was lower than that on the holiday.…”

Section: Relationship Between Cort and Saasupporting

confidence: 93%

Diurnal variation of salivary cortisol and amylase activity in fatigued state

Takahashi¹,

Yamaguchi²

2009

Environmental Health Risk V

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Saliva has great potential as a bio-fluid for point-of-use measurement of the human stress response. The purpose of this study was to evaluate the diurnal variation of salivary cortisol (CORT) and salivary amylase activity (SAA) under the condition of a fatigued state. The subjects were 11 emergency medical doctors who worked for the Department of Emergency and Critical Care Medicine in 24 h-shifts, and their diurnal variations were measured three times in one month during the 24h-shift workday. As a control experiment, 6 subjects' diurnal variations were measured during a holiday period. The saliva samples were collected 4 times in total during an examination. The mean CORT of a workday showed its lowest value of 1.07 ± 0.55 ng/ml before sleep (24:00), and was highest at 4.76 ± 3.16 ng/ml at the start of a daytime duty (9:00) (p < 0.01). In contrast, the mean CORT of a holiday showed its lowest value of 1.48 ± 1.00 ng/ml in the afternoon and a highest value of 4.74 ± 2.76 ng/ml the next morning (p < 0.05). The mean SAA of a workday showed its lowest value of 59.3 ± 31.5 kU/l during a daytime duty (12:00 -14:00) and was highest at 112.7 ± 90.1 kU/l at the start of night-time duty (18:00). These salivary biomarkers CORT and SAA on both workdays and holidays showed diurnal variation. In particular, the diurnal variation of CORT followed a well-defined 'bath-tub' shaped curve. A negative correlation of -0.36 was observed between the individual's CORT and SAA in workdays and of -0.62 in holidays, as determined by using the dynamic programming method. It was suggested that the fatigued state of humans is possibly reflected in the diurnal variations of CORT and SAA.

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Section: Relationship Between Cort and Saasupporting

confidence: 93%

Diurnal variation of salivary cortisol and amylase activity in fatigued state

Takahashi¹,

Yamaguchi²

2009

Environmental Health Risk V

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“…Those reports suggest that concentrations do tend to remain stable. Strong correlations over time have been reported for parotid saliva densitometric patterns (Oberg et al, 1982), stimulated parotid saliva concentrations of total protein, lysozyme, lactoferrin, and peroxidase , and unstimulated whole saliva concentrations of amylase, total protein, lysozyme, lactoferrin, peroxidase, and slgA Jenzano et al, 1987;Rudney et al, 1993). There is some uncertainty regarding the stability of slgA, since studies have reported conflicting results (Claman et al, 1967;Stuchell and Mandel, 1978;Burgio et al, 1980;Widerstrom and Bratthall, 1984;Bratthall and Widerstrom, 1985;Rudney et al, , 1993.…”

Section: (B) Longitudinal Variationmentioning

confidence: 99%

Does Variability in Salivary Protein Concentrations Influence Oral Microbial Ecology and Oral Health?

Rudney

1995

Critical Reviews in Oral Biology & Medicine

114

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Salivary protein interactions with oral microbes in vitro include aggregation, adherence, cell-killing, inhibition of metabolism, and nutrition. Such interactions might be expected to influence oral ecology. However, inconsistent results have been obtained from in vivo tests of the hypothesis that quantitative variation in salivary protein concentrations will affect oral disease prevalence. Results may have been influenced by choices made during study design, including saliva source, stimulation status, control for flow rate, and assay methods. Salivary protein concentrations also may be subject to circadian variation. Values for saliva collected at the same time of day tend to remain consistent within subjects, but events such as stress, inflammation, infection, menstruation, or pregnancy may induce short-term changes. Long-term factors such as aging, systemic disease, or medication likewise may influence salivary protein concentrations. Such sources of variation may increase the sample size needed to find statistically significant differences. Clinical studies also must consider factors such as human population variation, strain and species differences in protein-microbe interactions, protein polymorphism, and synergistic or antagonistic interaction between proteins. Salivary proteins may form heterotypic complexes with unique effects, and different proteins may exert redundant effects. Patterns of protein-microbe interaction also may differ between oral sites. Future clinical studies must take those factors into account. Promising approaches might involve meta-analysis or multi-center studies, retrospective and prospective longitudinal designs, short-term measurement of salivary protein effects, and consideration of individual variation in multiple protein effects such as aggregation, adherence, and cell-killing.

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“…There is little variation caused by aging, but daily fluctuations occur in salivary alpha-amylase activity 35,36) . For this reason, this examination was performed at the same time each day (15:00 to 17:00).…”

Section: Discussionmentioning

confidence: 99%

Intraoral electric potential <i>via</i> oral bacterial power generation —A novel mechanism of biofilm formation

et al. 2017

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In the early stages of biofilm accumulation, the electric charge of the dental enamel and pellicle surfaces is known to be involved. We therefore investigated the relationship between oral hygiene and intraoral electric potential (IoP) in 45 male participants using a double-blind study. IoP, but not body surface electric potential, was loosely correlated with oral hygiene condition (Oral Hygiene Index; OHI). IoP was also loosely correlated with smartphone use; however, there was no significant correlation between smartphone use and OHI. IoP elevation might be caused by OHI elevation resulting from biofilm formation as an internal factor, with smartphone use as an external factor. This in vitro study revealed the generating capacity of Streptococcus mutans accompanied by biofilm accumulation using a microbial fuel cell. These results suggest that IoP elevation is caused by biofilm accumulation induced by power generation of oral bacteria, resulting in elevation of OHI.

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Temporal variations of glandular kallikrein, protein and amylase in mixed human saliva

Cited by 38 publications

References 14 publications

Diurnal variation of salivary cortisol and amylase activity in fatigued state

Diurnal variation of salivary cortisol and amylase activity in fatigued state

Does Variability in Salivary Protein Concentrations Influence Oral Microbial Ecology and Oral Health?

Intraoral electric potential <i>via</i> oral bacterial power generation —A novel mechanism of biofilm formation

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