Temporally resolved direct delivery of second messengers into cells using nanostraws

Xu, Alexander M.; Kim, Sally A.; Wang, Derek S.; Aalipour, Amin; Melosh, Nicholas A.

doi:10.1039/c6lc00463f

Cited by 24 publications

(22 citation statements)

References 41 publications

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“…In comparison, no GFP signal was detected even 25 min after introducing LFN mixture to cells, showing much slower mRNA accessibility kinetics. The rate of mRNA availability is consistent with the observed temporal control for small-molecule delivery, which has been shown to be quite fast for the NS approach ( 32 , 33 ).…”

Section: Resultssupporting

confidence: 79%

Universal intracellular biomolecule delivery with precise dosage control

et al. 2018

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Section: Resultssupporting

confidence: 79%

Universal intracellular biomolecule delivery with precise dosage control

et al. 2018

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“…As substrates for cell culture, nanostraws (and related nanowires) are structurally robust and generally non‐toxic, although some perturbation in cell behavior has been observed . Previous applications of nanostraws have included the delivery of small molecules, DNA, membrane impermeable dyes, and ions …”

Section: Resultsmentioning

confidence: 99%

Direct Intracellular Delivery of Cell‐Impermeable Probes of Protein Glycosylation by Using Nanostraws

Wang

Shieh

et al. 2017

ChemBioChem

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Abstract:Bioorthogonal chemistry is an effective tool for measuring metabolic pathways and cellular activity, yet its use is currently limited due to the difficulty of introducing probes past the cell membrane and into the cytoplasm, especially as more complex probes are desired. Here we present a simple and minimally perturbative technique to deliver functional probes of glycolysylation into cells using a nanostructured "nanostraw" delivery system. Nanostraws provide large scale intracellular access to cells through fluidic conduits that remain small enough to minimize cell perturbation. First, we demonstrate that our platform can deliver an unmodified azidosugar, N-azidoacetylmannosamine, into cells with similar effectiveness as a chemical modification strategy (peracetylation). We then show that for an azidosugar modified with a charged uridine diphosphate group (UDP) that prevents intracellular penetration, the nanostraw platform enables its direct delivery into cells, thus bypassing multiple enzymatic processing steps. By effectively removing the cell permeability requirement from the probe, the nanostraws expand the toolbox of bioorthogonal probes to study biological processes using a single, easy-to-use platform.

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“…Challenged by these problems, several groups have developed alternative delivery platforms based on hollow nanoneedles to achieve precise biomanipulation with more control. [ 173–175 ]…”

Section: Intracellular Applicationsmentioning

confidence: 99%

Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane

He¹,

Hu²,

et al. 2020

Adv Funct Materials

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Establishing techniques to efficiently and nondestructively access the intracellular milieu is essential for many biomedical and scientific applications, ranging from drug delivery, to electrical recording, to biochemical detection. Cell penetration using nanoneedle arrays is currently a research focus area because it not only meets the increasing therapeutic demands of cell modifications and genome editing, but also provides an ideal platform for tracking long-term intracellular information. Although the precise mechanism driving membrane penetration by nanoneedle arrays is still unclear, the low cytotoxicity, wide range of delivered materials, diverse cell type targets, and simple material structures of nanoneedle arrays make these splendid platforms for cell access. Here, the recent progress in this field is reviewed by examining device architectures and discussing mechanisms for nanoneedle penetration, and the major studies demonstrating the most general applicability of nanoneedle arrays, typical methodologies to access the intracellular environment using nanoneedles with spontaneous or assisted penetration modes, as well as biosafety aspects are presented. This review should be valuable for deeply understanding the materials fabrication principles, device designs, cell penetration methodologies, biosafety aspects, and application strategies of nanoneedle array-based systems that are of crucial importance for the development of future practical biomedical platforms.

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Temporally resolved direct delivery of second messengers into cells using nanostraws

Cited by 24 publications

References 41 publications

Universal intracellular biomolecule delivery with precise dosage control

Universal intracellular biomolecule delivery with precise dosage control

Direct Intracellular Delivery of Cell‐Impermeable Probes of Protein Glycosylation by Using Nanostraws

Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane

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