Temporary immersion system for in vitro propagation via organogenesis of forest plant species

García-Ramírez, Yudith

doi:10.1007/s00468-022-02379-w

Cited by 10 publications

(5 citation statements)

References 67 publications

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“…According to their findings, increased CO 2 during plant propagation considerably enhanced the development of cocoa and yam propagules and eliminated the requirement for added sugars in the tissue culture growth medium. The designs and operations of various TISs are briefly described here, and detailed configurations of TIS, principles, and technological advances have been presented in previous reviews ( Etienne and Berthouly, 2002 ; Afreen, 2006 ; Watt, 2012 ; Georgiev et al., 2014 ; Garcia-Ramirez, 2023 ).…”

Section: Bioreactor Systems For Plant Propagationmentioning

confidence: 99%

“…Conventional micropropagation is considered a labor-intensive and costly technology because it involves the maintenance of a large number of culture vessels, semi-solid media, and periodic transfer of plant material to fresh media after subculturing for 4–6 weeks owing to the exhaustion of nutrients in the medium ( Maene and Debergh, 1985 ). Gelling agents substantially raise the expense of in vitro production and restrict the extent to which commercial propagation may be automated ( Garcia-Ramirez, 2023 ). Scaled-up and automated methods are consequently preferable to reduce handling throughout the processes necessary for micropropagation, boost multiplication rates, and overcome or minimize production costs ( Watt, 2012 ; Garcia-Ramirez, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

“…Gelling agents substantially raise the expense of in vitro production and restrict the extent to which commercial propagation may be automated ( Garcia-Ramirez, 2023 ). Scaled-up and automated methods are consequently preferable to reduce handling throughout the processes necessary for micropropagation, boost multiplication rates, and overcome or minimize production costs ( Watt, 2012 ; Garcia-Ramirez, 2023 ). Therefore, the use of liquid medium and culture of propagules in bioreactors have evolved as attractive alternatives to conventional propagation methods.…”

Section: Introductionmentioning

confidence: 99%

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Bioreactor systems for micropropagation of plants: present scenario and future prospects

et al. 2023

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Plant micropropagation has been adapted in the fields of agriculture, horticulture, forestry, and other related fields for large-scale production of elite plants. The use of liquid media and adoption of bioreactors have escalated the production of healthy plants. Several liquid-phase, gas-phase, temporary immersion, and other modified bioreactors have been used for plant propagation. The design, principle, operational mode, merits, and demerits of various bioreactors used for the regeneration of propagules, such as bulblets, cormlets, rhizomes, microtubers, shoots (subsequent rooting), and somatic embryos, are discussed here. In addition, various parameters that affect plant regeneration are discussed with suitable examples.

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Section: Bioreactor Systems For Plant Propagationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioreactor systems for micropropagation of plants: present scenario and future prospects

et al. 2023

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“…Studies have indicated that it facilitates faster plant multiplication and is more effective for producing certain secondary metabolites compared to plants grown in soil [ 5 , 13 , 14 , 15 , 16 ]. The key aspect for maximizing the potential of in vitro cultures maintained in the PlantForm TIS is the experimental optimization of process parameters [ 4 , 5 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Assessment of Lignan Profiling and Biological Activities of Schisandra henryi Leaf and In Vitro PlantForm Bioreactor-Grown Culture Extracts

Jafernik,

Kubica,

Dziurka

et al. 2024

Pharmaceuticals

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This research’s scope encompassed biotechnological, phytochemical, and biological studies of Schisandra henryi, including investigations into its in vitro microshoot culture grown in PlantForm bioreactors (temporary immersion systems, TISs), as well as extracts from leaves of the parent plant, focusing on anti-inflammatory, antioxidant, anticancer, and antimicrobial activities. The phytochemical analysis included the isolation and quantification of 17 compounds from dibenzocyclooctadiene, aryltetralin lignans, and neolignans using centrifugal partition chromatography (CPC), HPLC-DAD, and UHPLC-MS/MS tandem mass spectrometry with triple quadrupole mass filter methods. Higher contents of compounds were found in microshoots extracts (max. 543.99 mg/100 g DW). The major compound was schisantherin B both in the extracts from microshoots and the leaves (390.16 and 361.24 mg/100 g DW, respectively). The results of the anti-inflammatory activity in terms of the inhibition of COX-1, COX-2, sPLA2, and LOX-15 enzymes indicated that PlantForm microshoot extracts showed strong activity against COX-1 and COX-2 (for 177 mg/mL the inhibition percentage was 76% and 66%, respectively). The antioxidant potential assessed using FRAP, CUPRAC, and DPPH assays showed that extracts from microshoot cultures had 5.6, 3.8, and 3.3 times higher power compared to extracts from the leaves of the parent plant, respectively. The total polyphenol content (TPC) was 4.1 times higher in extracts from the in vitro culture compared to the leaves. The antiproliferative activity against T-cell lymphoblast line Jurkat, breast adenocarcinoma cultures (MCF-7), colon adenocarcinoma (HT-29), and cervical adenocarcinoma (HeLa), showed that both extracts have considerable effects on the tested cell lines. The antimicrobial activity tested against strains of Gram-positive and Gram-negative bacteria and fungi showed the highest activity towards H. pylori (MIC and MBC 0.625 mg/mL).

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“…In the same way, for biotic stresses, a T5 clone can be capable of growing throughout in insect stress areas when it is high above 2-3 m from the ground, such as tolerance to a gall wasp in particular genus Leptocybe (Kumar et al 2015;Mphahlele et al 2021) Furthermore, they can tolerate to leaf spot, leaf blight, and rust disease. Moreover, E. camaldulensis is excellent for propagation in cutting and in vitro culture (Mendonça et al 2016;García-Ramírez 2023).…”

Section: Introductionmentioning

confidence: 99%

Comparative of the complete chloroplast genome and RNA editing of Eucalyptus camaldulensis T5 clone, an elite variety in Thailand

RACHARAK,

SUTTANGKAKUL,

VUTTIPONGCHAIKIJ

2023

Biodiversitas

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Abstract. Racharak P, Suttangkakul A, Vuttipongchaikij S. 2023. Comparative of the complete chloroplast genome and RNA editing of Eucalyptus camaldulensis T5 clone, an elite variety in Thailand. Biodiversitas24: 3774-3784. Eucalyptus camaldulensis, T5 clone, the excellent fast-growing tree plantation in Thailand, was analyzed for the complete chloroplast genome and RNA editing. The complete chloroplast genome revealed a total genome size of 160,204 bp that divided into a large single copy (LSC) (88,904 bp) and a small single copy region (SSC) (18,506 bp) by inverted repeat regions (IR) containing 26,397 bp. A circular mapping genome and gene order showed the circle antiparallel mapping gene of 135 genes, including 37 tRNAs, 10 rRNAs, and 1 pseudogene. GC content of the genome was 36.87%. The comparative genomes analysis between the T5 clone and E. camaldulensis from the NCBI database suggested that the thymine (T) and adenine (A) strongly impacted the indel and transversion process, which could be a point of mutation in the genome. Furthermore, 24 specific genes were used to investigate RNA editing. From all genes, only 11 genes were edited with C to U conversion.Triplet codons, tUA, tUt, tUg and Ugg were the most frequently edited codon and expressions; the crucial influence of amino acid alterations. Due to RNA editing events, the physicochemical properties of amino acids were changed from polar to nonpolar amino acids and from hydrophilic to hydrophobic amino acids. Physicochemical properties conversion is necessary to form complete amino acid sequences for several essential chloroplast proteins. The event might be the accumulation of amino acid alterations causing phenotypic variation for plant adaptation and evolution.

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Temporary immersion system for in vitro propagation via organogenesis of forest plant species

Cited by 10 publications

References 67 publications

Bioreactor systems for micropropagation of plants: present scenario and future prospects

Bioreactor systems for micropropagation of plants: present scenario and future prospects

Comparative Assessment of Lignan Profiling and Biological Activities of Schisandra henryi Leaf and In Vitro PlantForm Bioreactor-Grown Culture Extracts

Comparative of the complete chloroplast genome and RNA editing of Eucalyptus camaldulensis T5 clone, an elite variety in Thailand

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