Temporary storage solution for adipose derived mesenchymal stem cells

Nofianti, Chyara Elisa; Sari, Intan Novita; Marlina, Marlina; Novialdi,; Pawitan, Jeanne Adiwinata

doi:10.21037/sci.2018.05.04

Cited by 9 publications

(13 citation statements)

References 15 publications

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“…The handling time until administration will have an impact on the survival and function of MSC. Survival of MSC is affected by the composition of storage media (31) and cytokine secretion profile of MSC can be altered, affecting MSC properties such as angiogenic potential (32). Our results show that the particular perfusion fluid used for our experiment is not detrimental for the secretion of angiogenic factors by hMSC.…”

Section: Discussionmentioning

confidence: 99%

“…Attachment of MSC to endothelial cells was shown to be reduced under flow compared to static conditions (33). Being in suspension in different clinically used storage solutions such as physiologic saline can influence the survival of MSC (31) and the composition of the solution that MSC are kept in modifies their metabolism and function (34, 35). Therefore, it can be deducted that the effect of MSC therapy delivered to renal grafts during ex vivo NMP will depend, among other factors, upon the composition of the perfusion fluid.…”

Section: Discussionmentioning

confidence: 99%

“…Mitochondrial activity, however, was similar in MSC in suspension and attached, suggesting that MSC are active also in suspension. Proliferation of pMSC was increased after exposure to perfusion fluid which could be a response to a specific component of the perfusion fluid (31). These results suggest that the nature of MSC make them more vulnerable when they are in suspension and therefore, it should be minimized when they are administered as therapy.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Normothermic Machine Perfusion Conditions on Mesenchymal Stromal Cells

et al. 2019

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Ex-situ normothermic machine perfusion (NMP) of transplant kidneys allows assessment of kidney quality and targeted intervention to initiate repair processes prior to transplantation. Mesenchymal stromal cells (MSC) have been shown to possess the capacity to stimulate kidney repair. Therefore, the combination of NMP and MSC therapy offers potential to repair transplant kidneys. It is however unknown how NMP conditions affect MSC. In this study the effect of NMP perfusion fluid on survival, metabolism and function of thawed cryopreserved human (h)MSC and porcine (p)MSC in suspension conditions was studied. Suspension conditions reduced the viability of pMSC by 40% in both perfusion fluid and culture medium. Viability of hMSC was reduced by suspension conditions by 15% in perfusion fluid, whilst no differences were found in survival in culture medium. Under adherent conditions, survival of the cells was not affected by perfusion fluid. The perfusion fluid did not affect survival of fresh MSC in suspension compared to the control culture medium. The freeze-thawing process impaired the survival of hMSC; 95% survival of fresh hMSC compared to 70% survival of thawed hMSC. Moreover, thawed MSC showed increased levels of reactive oxygen species, which indicates elevated levels of oxidative stress, and reduced mitochondrial activity, which implies reduced metabolism. The adherence of pMSC and hMSC to endothelial cells was reduced after the thawing process, effect which was particularly profound in in the perfusion fluid. To summarize, we observed that conditions required for machine perfusion are influencing the behavior of MSC. The freeze-thawing process reduces survival and metabolism and increases oxidative stress, and diminishes their ability to adhere to endothelial cells. In addition, we found that hMSC and pMSC behaved differently, which has to be taken into consideration when translating results from animal experiments to clinical studies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effects of Normothermic Machine Perfusion Conditions on Mesenchymal Stromal Cells

et al. 2019

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“…The storage condition is important to ensure the quality of ASCs preparation which can affect the outcome of therapy. The long-term, as well as short-term storage conditions of ASCs, have been investigated [72,73,74]. The media and temperature were optimized for enhancement of ASCs viability before isolation and application [72].…”

Section: Processes In the Storage Of Ascsmentioning

confidence: 99%

Adipose Tissue Stem Cells for Therapy: An Update on the Progress of Isolation, Culture, Storage, and Clinical Application

Chu

Phuong

Tien

et al. 2019

JCM

125

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Adipose tissue stem cells (ASCs), known as multipotent stem cells, are most commonly used in the clinical applications in recent years. Adipose tissues (AT) have the advantage in the harvesting, isolation, and expansion of ASCs, especially an abundant amount of stem cells compared to bone marrow. ASCs can be found in stromal vascular fractions (SVF) which are easily obtained from the dissociation of adipose tissue. Both SVFs and culture-expanded ASCs exhibit the stem cell characteristics such as differentiation into multiple cell types, regeneration, and immune regulators. Therefore, SVFs and ASCs have been researched to evaluate the safety and benefits for human use. In fact, the number of clinical trials on ASCs is going to increase by years; however, most trials are in phase I and II, and lack phase III and IV. This systemic review highlights and updates the process of the harvesting, characteristics, isolation, culture, storage, and application of ASCs, as well as provides further directions on the therapeutic use of ASCs.

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“…One type of adult stem cells that are widely used is the mesenchymal stem cell. Mesenchymal stem cells (MSCs) can be isolated easily from various tissues, including bone marrow [11], adipose tissue [12], umbilical cord [13], dental pulp [14], etc. Bone marrow MSCs were the first studied, followed by adipose tissue, umbilical cord, and various other tissues.…”

Section: The Properties Of Stem Cellsmentioning

confidence: 99%

Prospect of Stem Cell Therapy to Avoid Cytokine Storm in Severe Covid-19

Pawitan

2020

Int J App Pharm

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Severe COVID-19 cases are mostly due to severe inflammatory reaction and cytokine storm, which may lead to multiple organ failure and death. Until recently, there is no proven effective treatment for severe COVID-19. Mesenchymal stem cells (MSCs) have anti-inflammatory and regenerative properties. Therefore, they are supposed to work on COVID-19, which has failed to recover using other treatments. Therefore, studies are needed to determine the best tissue source of MSCs, the dose, repeat, and route of administration. For this review, we searched various databases, i.e. Pubmed, Science Direct, Springer, and WHO website using keywords: “mesenchymal stem cells” and “COVID-19” at 7 May 2020, without time limits. Various clinical trials on the use of MSCs for COVID-19 were registered, and initial results were reported. Initial results were promising but should be interpreted cautiously, as one was a case report, another one was case series, and one was a preliminary study of seven treated patients compared to three controls, where the baseline conditions were unequal. Therefore, well design randomized clinical trials are needed to get more robust prove.

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Temporary storage solution for adipose derived mesenchymal stem cells

Abstract: For our AT-MSCs, physiologic saline was superior to DMEM, and storage should not exceed 24 hours.

Cited by 9 publications

References 15 publications

Effects of Normothermic Machine Perfusion Conditions on Mesenchymal Stromal Cells

Effects of Normothermic Machine Perfusion Conditions on Mesenchymal Stromal Cells

Adipose Tissue Stem Cells for Therapy: An Update on the Progress of Isolation, Culture, Storage, and Clinical Application

Prospect of Stem Cell Therapy to Avoid Cytokine Storm in Severe Covid-19

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