Temporospatial characterization of the bronchus associated lymphoid tissue (BALT) of the one humped camel (Camelus dromedarius)

Elhussieny, Omnya; Zidan, Mohamed

doi:10.1007/s11250-021-02694-3

Cited by 4 publications

(2 citation statements)

References 43 publications

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“…In the present work, the frequency of MHCII+ and WC1+ lymphocytes in lymph nodes compared to blood indicates a similar tissue distribution pattern of B cells and γδ T cells in camel and bovine ( 21 ). All these results support previous reports on the similarity between the camel immune system and the immune system of other ruminants ( 26 ). Although mesenteric and bronchial lymph nodes showed similar frequency of lymphoid and monocytic cells as well as of helper and γδ T cell subsets, the lower fraction of B cells in the mesenteric lymph nodes indicates a tissue-specific distribution of these cells in the camel.…”

Section: Discussionsupporting

confidence: 92%

Flow cytometric analysis of immune cell populations in the bronchial and mesenteric lymph nodes of the dromedary camel

Hussen,

Althagafi,

Al-Sukruwah

et al. 2024

Front. Vet. Sci.

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Dromedary camel is an important livestock species with special economic value in arid and semi-arid regions of the world. Given the limited data on detailed immune cell composition and cell marker expression in the dromedary camel lymph node tissue, the present study was undertaken to investigate the immune cell composition of bronchial and mesenteric lymph nodes from healthy dromedary camels using flow cytometry. In this study, we applied flow cytometry and multicolor immuno-fluorescence to phenotype the main populations of immune cells in the bronchial and mesenteric camel lymph nodes and compared them with separated peripheral blood mononuclear cells and granulocytes. We used antibodies to detect several cell surface molecules associated with camel T cells (CD4, WC1), B cells (MHCII, BAQ44A), monocytes/macrophages (CD172a, CD14, CD163), in addition to the pan-leukocyte marker CD45 and the cell adhesion molecules CD44 and CD18. Compared to blood mononuclear cells, camel lymph node cells contained a higher percentage of lymphoid cells with only a minor fraction of myeloid cells. In addition, the lower expression of CD44 and CD18 on lymph node lymphocytes compared to lymphocytes from peripheral blood indicates higher frequency of naïve lymphocytes in the lymph nodes. The frequency of CD4+ T cells, B cells and γδ T cells within camel lymph node lymphocytes compared to blood indicates a similar tissue distribution pattern of lymphocyte subsets in camel and bovine and supports previous reports on the similarity between the camel immune system and the immune system of other ruminants. Lymph node neutrophils were identified as CD45++ CD172a++, CD14+, MHCIIlow, BAQ44A+, CD44++, CD18++ cells. In conclusion, the present study is describing the employment of flow cytometric single-cell analysis and immunostaining for the analysis of the immune cell composition in the camel lymph node.

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Section: Discussionsupporting

confidence: 92%

Flow cytometric analysis of immune cell populations in the bronchial and mesenteric lymph nodes of the dromedary camel

Hussen,

Althagafi,

Al-Sukruwah

et al. 2024

Front. Vet. Sci.

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“…This mainly includes the expression of the polymeric immunoglobulin receptor [38] in Bactrian camel lungs. In addition, two recent works characterized the anatomical distribution of the bronchus-associated lymphoid tissue in Bactrian camel [37] and dromedary camel lungs [39]. However, a flow cytometry procedure for the analysis of leukocyte populations in camel lung tissue is currently lacking.…”

Section: Discussionmentioning

confidence: 99%

Flow Cytometric Analysis of Leukocyte Populations in the Lung Tissue of Dromedary Camels

Hussen

Shawaf

Humam

et al. 2022

Veterinary Sciences

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Respiratory tract infections are among the most common infections in dromedary camels, with a high impact on animal health, production, and welfare. Tissue-specific distribution of immune cells is one of the important factors that influence the nature and outcome of the immune response to pathogens. Several protocols have recently been described for the flow cytometric analysis of immune cells in the lung tissue of several species. However, no such protocol currently exists for dromedary camels. The aim of the present study was, therefore, to establish a flow cytometric protocol for the identification of immune cell populations in the camel lung tissue and the evaluation of some of their phenotypic and functional properties. Combined staining of camel lung leukocytes with monoclonal antibodies to the pan-leukocyte marker CD45 and the myeloid cell marker CD172a allowed the identification of myeloid cells (CD45+CD172a+) and lymphoid cells (CD45+CD172a−) in the lung of healthy camels. The cell adhesion molecules CD11a and CD18 were found in a higher abundance on myeloid cells compared to lymphoid cells. Based on their differential expression of the LPS receptor CD14, macrophages (CD172a+CD14high cells) were identified as the most abundant immune cell population in the camel lung tissue. In contrast to their dominance in camel peripheral blood, granulocytes (CD172a+CD14low) presented only a minor population in the lung tissue. The higher frequency of γδ T cells in the lung tissue than in peripheral blood suggests a role for these cells in the pulmonary immune system. Flow cytometric analysis of bacterial phagocytosis and ROS production upon bacterial stimulation revealed high antimicrobial activity of camel lung phagocytes, which was comparable with the antimicrobial activity of blood granulocytes. Comparative analysis of immune cell distribution between the cranial and caudal lobes of the camel lung revealed a higher frequency of granulocytes and a lower frequency of macrophages in the cranial compared to the caudal lung lobe. In addition, the higher frequency of cells expressing the M2 macrophage marker CD163 in the caudal lung tissue, with a slightly higher fraction of MHCII-positive cells (M1 phenotype) in the cranial lung tissue, may suggest the distribution of different macrophage subtypes in the different lobes of the camel lung. Such differences between lung lobes could influence the effectiveness of the immune response to infection or vaccination with respiratory pathogens. Collectively, the present study identified some similarities and differences between camels and other farm animals regarding the distribution of the main immune cell populations in their lungs. Further studies are required for comprehensive immunophenotyping of the cellular pulmonary immune system in camels.

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Tylopoda: Camels and llamas

Tizard

2023

Comparative Mammalian Immunology

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Temporospatial characterization of the bronchus associated lymphoid tissue (BALT) of the one humped camel (Camelus dromedarius)

Cited by 4 publications

References 43 publications

Flow cytometric analysis of immune cell populations in the bronchial and mesenteric lymph nodes of the dromedary camel

Flow cytometric analysis of immune cell populations in the bronchial and mesenteric lymph nodes of the dromedary camel

Flow Cytometric Analysis of Leukocyte Populations in the Lung Tissue of Dromedary Camels

Tylopoda: Camels and llamas

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