Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves

Guo, Ying; Wang, Tongli; Fu, Fangfang; Wang, Guibin

doi:10.3389/fgene.2020.589326

Cited by 23 publications

(7 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Flavonoids are important compounds closely related to the color of plant organs. Recent studies have also found that flavonoids can regulate key enzymes, participate in antioxidant defense, have antimicrobial activity, and increase plant biological and abiotic stress resistance [ 72 , 73 , 74 ]. In particular, flavonoids can scavenge oxygen free radicals produced in plants, known as reactive oxygen species (ROS) [ 75 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolome Analysis of a Late-Senescent Vegetable Soybean during Seed Development Provides New Insights into Degradation of Chlorophyll

Wang

Chen

et al. 2022

Antioxidants

View full text Add to dashboard Cite

(1) Background: Senescence represents the final stage of plant growth and development, which transfers nutrients to growing seeds and directly affects the yield and quality of crops. However, little is known about chlorophyll degradation in developing and maturing seeds, in contrast to leaf senescence; (2) Methods: RNA-Seq was used to analyze the differentially expressed genes of different late-senescent germplasms. A widely untargeted metabolic analysis was used to analyze differential metabolites. In addition, qRT-PCR was conducted to detect gene expression levels; (3) Results: Transcriptome analysis revealed that ZX12 seeds have a higher expression level of the chlorophyll synthesis genes in the early stage of maturity, compared with ZX4, and have a lower expression level of chlorophyll degradation genes in the late stage of maturity. Flavonoids were the primary differential metabolites, and ZX12 contains the unique and highest expression of three types of metabolites, including farrerol-7-O-glucoside, cyanidin-3-o-(6′-o-feruloyl) glucoside, and kaempferide-3-o-(6′-malonyl) glucoside. Among them, farrerol-7-O-glucoside and cyanidin-3-o-(6′-o-feruloyl) glucoside are flavonoid derivatives containing mono and dihydroxy-B-ring chemical structures, respectively; and (4) Conclusions: It is speculated that the two metabolites can slow down the degradation process of chlorophyll by scavenging oxygen-free radicals in the chloroplast.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolome Analysis of a Late-Senescent Vegetable Soybean during Seed Development Provides New Insights into Degradation of Chlorophyll

Wang

Chen

et al. 2022

Antioxidants

View full text Add to dashboard Cite

show abstract

“…RNA sequencing data were processed using Trimmomatic, differentially expressed genes (DEGs) were identified using DESeq R package functions estimate size factors and nbinom test, and enrichment(GO and KEGG pathway);was performed based on the hypergeometric distribution ( 24 ). In addition, the DEGs expression pattern was visualized and analyzed by Short Time-series Expression Miner (STEM) software ( 25 ).…”

Section: Methodsmentioning

confidence: 99%

Pretreatment with IPA ameliorates colitis in mice: Colon transcriptome and fecal 16S amplicon profiling

Hou

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

3-Indolepropionic acid (IPA) is a tryptophan metabolite that has anti-inflammatory properties. The present study try to investigate the phylactic effects of IPA on dextran sodium sulfate (DSS)-induced colitis mice. The results showed that IPA pretreatment ameliorated the DSS-induced decrease in growth performance, and intestinal damage and enhanced immunity in mice. RNA-seq analysis of mouse colon samples revealed that the differentially expressed genes (DEGs) were mainly enriched in immune-related pathways. 16S rRNA sequencing showed that IPA pretreatment ameliorated DSS-induced colonic microbiota dysbiosis. Moreover, the expression levels of gut immune genes were positively correlated with the relative abundance of several probiotics, such as Alloprevotella and Catenibacterium. In conclusion, IPA alleviates DSS-induced acute colitis in mice by regulating inflammatory cytokines, balancing the colonic microbiota and modulating the expression of genes related to inflammation, which would also provide a theoretical basis for IPA as a strategy to improve intestinal health.

show abstract

“… 80 , 81 GbbHLH, GbTrihelix , and three GbERFs contributed to flavonoid biosynthesis by regulating the expression of structural genes in the flavonoid biosynthesis pathway. 82 In recent years, some studies found that TCPs may also be involved in flavonoid biosynthesis. For instance, AtTCP15 affected anthocyanin accumulation by inhibiting the expression of anthocyanin biosynthesis genes, 38 and FvTCP9 promoted anthocyanin accumulation by affecting the expression of genes involved in the ABA signal transduction pathway.…”

Section: Candidate Gbtcps Involved In Flavonoids B...mentioning

confidence: 99%

Genome-wide identification and expression pattern analysis of the TCP transcription factor family in Ginkgo biloba

Chen

Zheng

et al. 2022

Plant Signaling & Behavior

View full text Add to dashboard Cite

Plant-specific TCP transcription factors play an essential role in plant growth and development. They can regulate leaf curvature, flower symmetry and the synthesis of secondary metabolites. The flavonoids in Ginkgo biloba leaf are one of the main medicinally bioactivate compounds, which have pharmacological and beneficial health effects for humans. In this study, a total of 13 TCP genes were identified in G. biloba , and 5 of them belonged to PCF subclades ( GbTCP03, GbTCP07, GbTCP05, GbTCP13, GbTCP02 ) while others belonged to CIN ( GbTCP01, GbTCP04, GbTCP06, GbTCP08, GbTCP09, GbTCP10, GbTCP11, GbTCP12 ) subclades according to phylogenetic analysis. Numerous cis -acting elements related to various biotic and abiotic signals were predicted on the promoters by cis -element analysis, suggesting that the expression of GbTCPs might be co-regulated by multiple signals. Transcript abundance analysis exhibited that most of GbTCPs responded to multiple phytohormones. Among them, the relative expression levels of GbTCP06, GbTCP11 , and GbTCP13 were found to be significantly influenced by exogenous ABA, SA and MeJA application. In addition, a total of 126 miRNAs were predicted to target 9 TCPs (including GbTCP01, GbTCP02, GbTCP04, GbTCP05, GbTCP06, GbTCP08, GbTCP11, GbTCP12, GbTCP13 ). The correlation analysis between the expression level of GbTCPs and the flavonoid contents showed that GbTCP03, GbTCP04, GbTCP07 might involve in flavonoid biosynthesis in G. biloba . In short, this study mainly provided a theoretical foundation for better understanding the potential function of TCPs in G. biloba .

show abstract

Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves

Cited by 23 publications

References 47 publications

Transcriptome and Metabolome Analysis of a Late-Senescent Vegetable Soybean during Seed Development Provides New Insights into Degradation of Chlorophyll

Transcriptome and Metabolome Analysis of a Late-Senescent Vegetable Soybean during Seed Development Provides New Insights into Degradation of Chlorophyll

Pretreatment with IPA ameliorates colitis in mice: Colon transcriptome and fecal 16S amplicon profiling

Genome-wide identification and expression pattern analysis of the TCP transcription factor family in Ginkgo biloba

Contact Info

Product

Resources

About