Ten-year Experiences on Initial Genetic Examination in Childhood Acute Lymphoblastic Leukaemia in Hungary (1993–2002). Technical Approaches and Clinical Implementation

Oláh, Éva; Balogh, Erzsébet; Pajor, László; Jakab, Zsuzsanna

doi:10.1007/s12253-010-9286-2

Cited by 4 publications

(2 citation statements)

References 34 publications

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“…None of the cases in our study had a DI value between1.06-1.09 (hyperdiploid A or Less hyperdiploidy) and < 0.90 (hypodiploid). Various studies from West quote a low incidence of hypodiploidy ranging from 0.3-7.6% (Olah et al, 2011;Woo et al, 2014), except for a study by (Khalifa et al, 1997), which shows an incidence of 11.4%. Incidence of less hyperdiploidy (DI 1.06-1.09) also varies widely in different studies ranging from 5%-16.3% (Raimondi et al, 1988;Raimondi et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

DNA Ploidy and S-phase Fraction Analysis in Paediatric B-cell Acute Lymphoblastic Leukemia Cases: a Tertiary Care Centre Experience

Kumar

Bhatia²,

Trehan³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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DNA ploidy is an important prognostic parameter in paediatric B-ALL, but the significance of the S-phase fraction is unclear. In present study, DNA ploidy was assessed in 40 pediatric B-ALL cases by flow cytometry. The DI (DNA index) and percentage of cells in S-phase were calculated using Modfit software. Aneuploidy was noted in 26/40 (65%) cases. A DI of 1.10-1.6 (hyperdiploidy B) was noted in 20/40 (50%) and 6/40 (15%) had a DI>1.60 (triploid and tetraploid range). Some 14/40 (35%) cases had a diploid DI between 0.90-1.05. None of the cases had a DI <0.90 (hypodiploid) or in the 1.06-1.09 (hyperdiploid A) range. The mean S-phase fraction was 2.6%, with 24/40 (60%) having low and 16/40 (40%) high S-phase fractions. No correlation was noted with standard ALL risk and treatment response factors with DI values or S-phase data, except for a positive correlation of low S-phase with high NCI risk category (p=0. 032). Overall frequency of hyperdiploidy in our cohort of B-ALL patients was very high (65%). No correlation between hyperdiploidy B and low TLC or common B-phenotypewas observed in our study as 42% cases with DI 1.10-1.6 had TLC> 50 x 10 9 and 57.1% CD 10 negativity. The study also highlighted that S-phase fraction analysis does not add any prognostic information and is not a useful parameter for assessment in ALL cases. However, larger studies with long term outcome analysis are needed to derive definitive conclusions.

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Section: Discussionmentioning

confidence: 99%

DNA Ploidy and S-phase Fraction Analysis in Paediatric B-cell Acute Lymphoblastic Leukemia Cases: a Tertiary Care Centre Experience

Kumar

Bhatia²,

Trehan³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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“…These findings suggest that DI is a reliable and sensitive method for determining cell ploidy. Another study that analyzed 588 children diagnosed with ALL over 10 years indicated that the 3 techniques (karyotype, FISH, and DI) in combination helps to determine the correct genetic subgrouping, especially in the case of lack of successful karyotyping, or if the cells have more than one cell line [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Accuracy of Flow Cytometric DNA Index in Saudi Children with B Cell Acute Lymphoblastic Leukemia

Alatawi,

Bakhsh,

Tashkandi

et al. 2023

Children

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B cell Acute Lymphoblastic Leukemia (B-ALL) is one of the most common types of cancer diagnosed in children in Saudi Arabia. Cytogenetic investigations, including karyotyping and FISH, are used to determine the incidence and prognostic significance of chromosomal abnormalities in B-ALL. However, in ALL, accurate identification of the morphology of chromosomes is sometimes not achieved. Flow cytometric DI may have the advantage of being technically fast, using either fresh or frozen samples to correctly stratify the patient into the appropriate risk group for treatment. In this study, we evaluated the reliability and validity of using fixed samples instead of fresh samples to determine aneuploidy in cancer cells using a DNA index. The results of the DNA index obtained by flow cytometry were compared with those of conventional cytogenetic analysis to validate the accuracy. Fixed samples (n = 72) from children diagnosed with B-ALL at King Fahad Medical City in Riyadh between 2017 and 2019 were investigated. The results showed strong and statistically significant positive correlation between DNAI-FCM and conventional cytogenetic analysis (p = 0.000 < 0.01). The DNA index value by flow cytometry was proportional to the cytogenetic study in 94.36% (67) of the cases, while discrepancy was observed in 5.6% (four) cases. Our findings highlight the ability of the DNA index method to provide complementary information for the accurate diagnosis of aneuploidy in patients with B-ALL.

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Singularities in Pediatric Bone Marrow Lymphoid Processes

Yohe

McKenna

2012

Bone Marrow Lymphoid Infiltrates

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Ten-year Experiences on Initial Genetic Examination in Childhood Acute Lymphoblastic Leukaemia in Hungary (1993–2002). Technical Approaches and Clinical Implementation

Abstract: Complementary use of each of genetic methods used is necessary for reliable genetic diagnosis according to the algorithm presented. Specific genetic alterations proved to be of prognostic significance.

Cited by 4 publications

References 34 publications

DNA Ploidy and S-phase Fraction Analysis in Paediatric B-cell Acute Lymphoblastic Leukemia Cases: a Tertiary Care Centre Experience

DNA Ploidy and S-phase Fraction Analysis in Paediatric B-cell Acute Lymphoblastic Leukemia Cases: a Tertiary Care Centre Experience

Diagnostic Accuracy of Flow Cytometric DNA Index in Saudi Children with B Cell Acute Lymphoblastic Leukemia

Singularities in Pediatric Bone Marrow Lymphoid Processes

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