Tenascin‐<scp>C</scp> promotes differentiation of rat dental pulp cells <i>in vitro</i>

Matsuoka, Kaichi; Matsuzaka, Kenichi; Yoshinari, Masao; Inoue, Takashi

doi:10.1111/j.1365-2591.2012.02089.x

Cited by 10 publications

(11 citation statements)

References 40 publications

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“…), actively participates in physiological mineralization (Mackie & Murphy ) and promotes the differentiation of rat dental pulp cells (Matsuoka et al . ). In the present study, TNC was found to be significantly elevated under SMG conditions, which indicates that SMG might also function as a promoter of the odontogenic differentiation of hDPSCs through a TNC‐mediated mechanism.…”

Section: Discussionmentioning

confidence: 97%

Increased proliferation and adhesion properties of human dental pulp stem cells in PLGA scaffolds via simulated microgravity

Pan

et al. 2015

Int Endodontic J

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Section: Discussionmentioning

confidence: 97%

Increased proliferation and adhesion properties of human dental pulp stem cells in PLGA scaffolds via simulated microgravity

Pan

et al. 2015

Int Endodontic J

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“…Common extracellular matrix molecules were selected for immunohistochemical analysis (17–19). Five-micrometer paraffin embedded sections were dewaxed, and treated with 3% hydrogen peroxide after antigen retrieval.…”

Section: Methodsmentioning

confidence: 99%

Extracellular diffusion quantified by magnetic resonance imaging during rat C6 glioma cell progression

Song

Luo

Dong

et al. 2017

Braz J Med Biol Res

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Solution reflux and edema hamper the convection-enhanced delivery of the standard treatment for glioma. Therefore, a real-time magnetic resonance imaging (MRI) method was developed to monitor the dosing process, but a quantitative analysis of local diffusion and clearance parameters has not been assessed. The objective of this study was to compare diffusion into the extracellular space (ECS) at different stages of rat C6 gliomas, and analyze the effects of the extracellular matrix (ECM) on the diffusion process. At 10 and 20 days, after successful glioma modeling, gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA) was introduced into the ECS of rat C6 gliomas. Diffusion parameters and half-life of the reagent were then detected using MRI, and quantified according to the mathematical model of diffusion. The main ECM components [chondroitin sulfate proteoglycans (CSPGs), collagen IV, and tenascin C] were detected by immunohistochemical and immunoblot analyses. In 20-day gliomas, Gd-DTPA diffused more slowly and derived higher tortuosity, with lower clearance rate and longer half-life compared to 10-day gliomas. The increased glioma ECM was associated with different diffusion and clearance parameters in 20-day rat gliomas compared to 10-day gliomas. ECS parameters were altered with C6 glioma progression from increased ECM content. Our study might help better understand the glioma microenvironment and provide benefits for interstitial drug delivery to treat brain gliomas.

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“…Primer sequence and size used in this study were shown in Table 2 . Quantification was performed by comparing the levels obtained with standard samples as a previous study [ 21 ]. In the present study, the concentrations of cDNA in the unstimulated samples were 0.2, 0.5, 1.0 and 2.0 μl.…”

Section: Methodsmentioning

confidence: 99%

HSP70 mRNA expression by cells of the epithelial rest of Malassez due to mechanical forces in vitro

et al. 2016

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BackgroundThe purpose of the present study was to examine the in vitro responses of ERM cells under the combination of centrifugal and compression forces, in terms of their expression of HSP70 mRNA.MethodsThe ERM cells were positive for CK19 indicating that they were derived from the odontogenic epithelium. Cultured ERM cells were applied centrifugal force and compressing force at one to three times as mechanical forces. After addition of forces, cells were observed using scanning electron microscope (SEM) and were measured expression of HSP70 mRNA by RT-PCR.ResultsSEM observations showed the cells were flattened immediately after the application of mechanical force, but nuclear protrusions recovered the same as the control 3 h later. A significantly higher expression of HSP70 mRNA was observed in ERM cells under mechanical force compared with the control, but it gradually decreased with time. No accumulation of HSP70 mRNA expression occurred with intermittent force. However, the expression of HSP70 mRNA with intermittent force repeated 3 times was significantly higher compared with intermittent force applied only once or twice.ConclusionsThese findings suggest that ERM cells express HSP70 mRNA in response to mechanical force, and that intermittent force maintains the level of HSP70 mRNA expression.

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Tenascin‐C promotes differentiation of rat dental pulp cells in vitro

Abstract: TN-C promoted differentiation of rat dental pulp cells by the activation of Notch.

Cited by 10 publications

References 40 publications

Increased proliferation and adhesion properties of human dental pulp stem cells in PLGA scaffolds via simulated microgravity

Increased proliferation and adhesion properties of human dental pulp stem cells in PLGA scaffolds via simulated microgravity

Extracellular diffusion quantified by magnetic resonance imaging during rat C6 glioma cell progression

HSP70 mRNA expression by cells of the epithelial rest of Malassez due to mechanical forces in vitro

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