Tendon healing and anti-adhesion properties of electrospun fibrous membranes containing bFGF loaded nanoparticles

Liu, Shen; Ming-jie, Qin; Hu, Changmin; Wu, Fei; Cui, Wenguo; Jin, Tao; Fan, Cunyi

doi:10.1016/j.biomaterials.2013.03.026

Cited by 149 publications

(119 citation statements)

References 32 publications

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“…A burst release of 15.4% was detected during the initial 2 days, followed by a constant release for 14 days (days 2-16) and then a slow release for another 8 days (days [16][17][18][19][20][21][22][23][24]. bFGF located on or near the fiber surface results in the burst release, and the encapsulation of bFGF into the fiber core can apparently alleviate the initial burst release.…”

Section: In Vitro Bfgf Release Profilesmentioning

confidence: 98%

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Biological augmentation of rotator cuff repair using bFGF-loaded electrospun poly(lactide-co-glycolide) fibrous membranes

Zhao¹,

Zhao²,

Dong³

et al. 2014

IJN

Self Cite

108

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Clinically, rotator cuff tear (RCT) is among the most common shoulder pathologies. Despite significant advances in surgical techniques, the re-tear rate after rotator cuff (RC) repair remains high. Insufficient healing capacity is likely the main factor for reconstruction failure. This study reports on a basic fibroblast growth factor (bFGF)-loaded electrospun poly(lactide-co-glycolide) (PLGA) fibrous membrane for repairing RCT. Implantable biodegradable bFGF–PLGA fibrous membranes were successfully fabricated using emulsion electrospinning technology and then characterized and evaluated with in vitro and in vivo cell proliferation assays and repairs of rat chronic RCTs. Emulsion electrospinning fabricated ultrafine fibers with a core-sheath structure which secured the bioactivity of bFGF in a sustained manner for 3 weeks. Histological observations showed that electrospun fibrous membranes have excellent biocompatibility and biodegradability. At 2, 4, and 8 weeks after in vivo RCT repair surgery, electrospun fibrous membranes significantly increased the area of glycosaminoglycan staining at the tendon–bone interface compared with the control group, and bFGF–PLGA significantly improved collagen organization, as measured by birefringence under polarized light at the healing enthesis compared with the control and PLGA groups. Biomechanical testing showed that the electrospun fibrous membrane groups had a greater ultimate load-to-failure and stiffness than the control group at 4 and 8 weeks. The bFGF–PLGA membranes had the highest ultimate load-to-failure, stiffness, and stress of the healing enthesis, and their superiority compared to PLGA alone was significant. These results demonstrated that electrospun fibrous membranes aid in cell attachment and proliferation, as well as accelerating tendon–bone remodeling, and bFGF-loaded PLGA fibrous membranes have a more pronounced effect on tendon–bone healing. Therefore, augmentation using bFGF–PLGA electrospun fibrous membranes is a promising treatment for RCT.

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Section: In Vitro Bfgf Release Profilesmentioning

confidence: 98%

“…Encapsulation efficiency (%) = (P/Pt) ×100, where P is the actual protein encapsulated into the membranes and Pt is the theoretical amount of protein encapsulated into the membranes. 24 …”

Section: Bfgf Encapsulationmentioning

confidence: 99%

Biological augmentation of rotator cuff repair using bFGF-loaded electrospun poly(lactide-co-glycolide) fibrous membranes

Zhao¹,

Zhao²,

Dong³

et al. 2014

IJN

Self Cite

108

View full text Add to dashboard Cite

show abstract

“…GDF-5 loaded PLGA electro-spun scaffolds supported growth and promoted tenogenic differentiation of rat ADSCs [418]. Further in vitro studies using bFGF loaded PLGA electro-spun scaffolds have demonstrated enhanced BMSC proliferation and tendon differentiation, as evidenced by increased expression collagen type I and tenascin-C [419], whilst in vivo studies have shown greater vascularisation, higher histological scores and superior mechanical properties to naturally healed and non-functionalised counterparts [420]. A more complex approach based on PLGA electro-spun scaffold and a fibrin gel loaded with ADSCs and PDGF-ββ demonstrated improved healing over standard controls [421].…”

Section: Bottom-up Approached For Tendon Repair Based On Synthetic Inmentioning

confidence: 99%

The past, present and future in scaffold-based tendon treatments

Lomas

Ryan

Sorushanova

et al. 2015

Advanced Drug Delivery Reviews

183

188

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“…A porous structure manufactured with a processing technique that can add to this versatility is required; this can be achieved with electrospinning. Electrospinning is an ideal technique for producing 3D networks of fibers of tunable size, orientation, composition, and density that mimic the properties of native extracellular matrix (ECM)1, 2, 3 and can generate scaffolds with spatially arranged functionalization through layering of various polymers during electrospinning 4, 5, 6, 7, 8, 9. Developing a controlled method that allows multiple zonally arranged functional groups within a continuous scaffold allows for the production of a hierarchical structure that can modulate cell behavior within each functional zone.…”

Section: Introductionmentioning

confidence: 99%

Modular and Versatile Spatial Functionalization of Tissue Engineering Scaffolds through Fiber‐Initiated Controlled Radical Polymerization

Harrison

Steele

Chapman

et al. 2015

Adv Funct Materials

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Native tissues are typically heterogeneous and hierarchically organized, and generating scaffolds that can mimic these properties is critical for tissue engineering applications. By uniquely combining controlled radical polymerization (CRP), end‐functionalization of polymers, and advanced electrospinning techniques, a modular and versatile approach is introduced to generate scaffolds with spatially organized functionality. Poly‐ε‐caprolactone is end functionalized with either a polymerization‐initiating group or a cell‐binding peptide motif cyclic Arg‐Gly‐Asp‐Ser (cRGDS), and are each sequentially electrospun to produce zonally discrete bilayers within a continuous fiber scaffold. The polymerization‐initiating group is then used to graft an antifouling polymer bottlebrush based on poly(ethylene glycol) from the fiber surface using CRP exclusively within one bilayer of the scaffold. The ability to include additional multifunctionality during CRP is showcased by integrating a biotinylated monomer unit into the polymerization step allowing postmodification of the scaffold with streptavidin‐coupled moieties. These combined processing techniques result in an effective bilayered and dual‐functionality scaffold with a cell‐adhesive surface and an opposing antifouling non‐cell‐adhesive surface in zonally specific regions across the thickness of the scaffold, demonstrated through fluorescent labelling and cell adhesion studies. This modular and versatile approach combines strategies to produce scaffolds with tailorable properties for many applications in tissue engineering and regenerative medicine.

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Tendon healing and anti-adhesion properties of electrospun fibrous membranes containing bFGF loaded nanoparticles

Cited by 149 publications

References 32 publications

Biological augmentation of rotator cuff repair using bFGF-loaded electrospun poly(lactide-co-glycolide) fibrous membranes

Biological augmentation of rotator cuff repair using bFGF-loaded electrospun poly(lactide-co-glycolide) fibrous membranes

The past, present and future in scaffold-based tendon treatments

Modular and Versatile Spatial Functionalization of Tissue Engineering Scaffolds through Fiber‐Initiated Controlled Radical Polymerization

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