Tendon Remodeling in Response to Resistance Training, Anabolic Androgenic Steroids and Aging

Guzzoni, Vinícius; Selistre-de-Araújo, Heloísa Sobreiro; Marqueti, Rita de Cássia

doi:10.3390/cells7120251

Cited by 18 publications

(15 citation statements)

References 174 publications

(283 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chronic tendon disorders, most commonly affecting the calcaneus tendon (CT), represents approximately 50% of all sports injuries (Barin et al, 2019). Moreover, CT has a relevant biomechanical function in response to RT, which justifies the choice of use CT (Guzzoni et al, 2018). The CT was immediately dissected from the posterior right paw and frozen in clean microtubes using liquid nitrogen and then stored at −80 • C. All tendons were dissected by an experienced researcher in order to prevent contamination of other tissues.…”

Section: Euthanasiamentioning

confidence: 99%

Paternal Resistance Training Modulates Calcaneal Tendon Proteome in the Offspring Exposed to High-Fat Diet

Neto

Tibana

Silva

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

The increase in high-energy dietary intakes is a well-known risk factor for many diseases, and can also negatively impact the tendon. Ancestral lifestyle can mitigate the metabolic harmful effects of offspring exposed to high-fat diet (HF). However, the influence of paternal exercise on molecular pathways associated to offspring tendon remodeling remains to be determined. We investigated the effects of 8 weeks of paternal resistance training (RT) on offspring tendon proteome exposed to standard diet or HF diet. Wistar rats were randomly divided into two groups: sedentary fathers and trained fathers (8 weeks, three times per week, with 8-12 dynamic movements per climb in a stair climbing apparatus). The offspring were obtained by mating with sedentary females. Upon weaning, male offspring were divided into four groups (five animals per group): offspring from sedentary fathers were exposed either to control diet (SFO-C), or to high-fat diet (SFO-HF); offspring from trained fathers were exposed to control diet (TFO-C) or to a high-fat diet (TFO-HF). The Nano-LC-MS/MS analysis revealed 383 regulated proteins among offspring groups. HF diet induced a decrease of abundance in tendon proteins related to extracellular matrix organization, transport, immune response and translation. On the other hand, the changes in the offspring tendon proteome in response to paternal RT were more pronounced when the offspring were exposed to HF diet, resulting in positive regulation of proteins essential for the maintenance of tendon integrity. Most of the modulated proteins are associated to biological pathways related to tendon protection and damage recovery, such as extracellular matrix organization and

show abstract

Section: Euthanasiamentioning

confidence: 99%

Paternal Resistance Training Modulates Calcaneal Tendon Proteome in the Offspring Exposed to High-Fat Diet

Neto

Tibana

Silva

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Vinicius revealed that in comparison to their levels in young animals, collagen-I (Col-I) content and glucosaminoglycans (GAGs) were reduced in the calcaneal tendon of older sedentary animals, while biglycan (Bgn) and fibromodulin (Fmod) content were not altered with aging. The gene expression levels of COL-1, COL-3, Insulin-Like Growth Factor-Ia (IGF-Ia), Matrix metalloproteinase-2(MMP-2), tissue inhibitor of metalloproteinase-2(TIMP-2), and Bgn were decreased in aged tendons [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of aging on the histology and biochemistry of rat tendon healing

Lai

Tang

Wang

et al. 2021

BMC Musculoskelet Disord

View full text Add to dashboard Cite

Introduction Tendon diseases and injuries are a serious problem for the aged population, often leading to pain, disability and a significant decline in quality of life. The purpose of this study was to determine the influence of aging on biochemistry and histology during tendon healing and to provide a new strategy for improving tendon healing. Method A total of 24 Sprague-Dawley rats were equally divided into a young and an aged group. A rat patellar tendon defect model was used in this study. Tendon samples were collected at weeks 2 and 4, and hematoxylin-eosin, alcian blue and immunofluorescence staining were performed for histological analysis. Meanwhile, reverse transcription-polymerase chain reaction (RT-PCR) and western blot were performed to evaluate the biochemical changes. Results The histological scores in aged rats were significantly lower than those in young rats. At the protein level, collagen synthesis-related markers Col-3, Matrix metalloproteinase-1 and Metallopeptidase Inhibitor 1(TIMP-1) were decreased at week 4 in aged rats compared with those of young rats. Though there was a decrease in the expression of the chondrogenic marker aggrecan at the protein level in aged tendon, the Micro-CT results from weeks 4 samples showed no significant difference(p>0.05) on the ectopic ossification between groups. Moreover, we found more adipocytes accumulated in the aged tendon defect with the Oil Red O staining and at the gene and protein levels the markers related to adipogenic differentiation. Conclusions Our findings indicate that tendon healing is impaired in aged rats and is characterized by a significantly lower histological score, decreased collagen synthesis and more adipocyte accumulation in patellar tendon after repair.

show abstract

“…The risk of trauma due to muscle tension is probably a confluence of numerous factors related to the MTJ's ultrastructural architecture and its contact surface 18,19 . Thus, physical training appears as an alternative to strengthen the muscle-tendon structure and ensure an effective transmission of force from the skeletal muscle to the tendon [20][21][22][23] . According to Curzi et al, MTJ plasticity seems to be related to exercise intensity 24 .…”

mentioning

confidence: 99%

Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche

Neto

Rocha

Barbosa

et al. 2020

Sci Rep

View full text Add to dashboard Cite

The present study shows chronic adjustments in the myotendinous junction (MTJ) in response to different ladder-based resistance training (LRT) protocols. Thirty adult male Wistar rats were divided into groups: sedentary (S), calisthenics (LRT without additional load [C]), and resistance-trained (LRT with extra weight [R]). We demonstrated longer lengths of sarcoplasmatic invaginations in the trained groups; however, evaginations were seen mainly in group R. We showed a greater thickness of sarcoplasmatic invaginations in groups C and R, in addition to greater evaginations in R. We also observed thinner basal lamina in trained groups. The support collagen layer (SCL) adjacent to the MTJ and the diameters of the transverse fibrils were larger in R. We also discovered a niche of telocytes in the MTJ with electron micrographs of the plantar muscle and with immunostaining with CD34+ in the gastrocnemius muscle near the blood vessels and pericytes. We concluded that the continuous adjustments in the MTJ ultrastructure were the result of tissue plasticity induced by LRT, which is causally related to muscle hypertrophy and, consequently, to the remodeling of the contact interface. Also, we reveal the existence of a collagen layer adjacent to MTJ and discover a new micro anatomic location of telocytes. The myotendinous junction (MTJ) consists of a highly specific anatomical region in which the sarcoplasmatic membranes connect to bundles of extracellular matrix (ECM) collagen fibers 1. Due to its functionality, MTJ represents the region with the highest transmission of force 2,3. Morphologically, MTJ presents projections that penetrate the muscle tissue, parallel and directed to the myofibrils, forming sarcoplasmatic invaginations. Currently, we know that its development is mainly influenced by the interactions between myoblasts and ECM elements 4,5. The basal lamina of muscle fiber creates a supramolecular connection structure composed of different levels of proteins from the laminin and collagen polymers located in the ECM 6. This arrangement contributes structurally to the transmission of force, adapting, remodeling, and consequently affecting the macroscopic tendon structure to different stimuli 7,8. Initially, telocytes were described as interstitial Cajal cells 9. Telocytes are interstitial cells of stromal origin with an oval shape, and a heterochromatic nucleus with moniliform projections denominated telopodes, which characterize its morphological classification according to the number of projections 9,10. They are founded in the interstitium of tissues such as the testicle 9 , smooth and cardiac muscle 11 , human tongue 10 , pancreas 12 , and liver 13. It can be identified by the electronic microscopy, immunohistochemistry, and CD34+/immunostaining 14. Recently, telocytes have been found in proximity to and engaging in possible interactions with satellite cells 10. The telocytes projections have terminals (pods) with mitochondria that form junctions with adjacent cells, and they have caveolae that perform exocytos...

show abstract

Tendon Remodeling in Response to Resistance Training, Anabolic Androgenic Steroids and Aging

Cited by 18 publications

References 174 publications

Paternal Resistance Training Modulates Calcaneal Tendon Proteome in the Offspring Exposed to High-Fat Diet

Paternal Resistance Training Modulates Calcaneal Tendon Proteome in the Offspring Exposed to High-Fat Diet

Effects of aging on the histology and biochemistry of rat tendon healing

Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche

Contact Info

Product

Resources

About