Tenebrionicola larvae gen. nov., sp. nov., isolated from larvae of mealworm Tenebrio molitor L., and a proposal to transfer Erwinia teleogrylli Liu et al. 2016 to a new genus Entomohabitans as Entomohabitans teleogrylli comb. nov.

Abstract

Two enterobacterial strains, designated YMB-R21T and YMB-R22, were isolated from larvae of mealworm Tenebrio molitor L. and examined for their taxonomic characteristics. A 16S rRNA gene-based neighbour-joining tree showed that the two isolates formed two distinct sublineages within the family Enterobacteriaceae and were separated from other genera of the family. The isolates showed 16S rRNA gene sequence similarity of 98.9 % to each other and ≤96.5 % to members of the order Enterobacteriales . The phylogenomic analysis based on 92 singly-copy core genes showed that the two isolates belonged to the family Enterobacteriaceae and formed a distinct sublineage at a position located remotely from the genera of the family. The loosely associated members were the type strain of Erwinia teleogrylli and members of the genus Shimwellia . Average nucleotide identity and digital DNA–DNA hybridization values showed that the isolates represented members of a novel species in the family Enterobacteriaceae . The values of amino acid identity between the two isolates and the closest relatives were 74.5–75.0 % with the type strain of E. teleogrylli and 74.5–74.8 % with the type strains of two Shimwellia species, while E. teleogrylli showed the amino acid identity values of 76.3–76.5 % with two Shimwellia species. Based on the results obtained here, we propose a new genus Tenebrionicola with the description of Tenebrionicola larvae sp. nov. (type strain YMB-R21T=KCTC 82597T=CCM 9152T and strain YMB-R22=KCTC 82598=CCM 9153), with the transfer of Erwinia teleogrylli Liu et al. 2016 to a new genus Entomohabitans as Entomohabitans teleogrylli comb. nov. (type strain SCU-B244T=CGMCC 1.12772T=DSM 28222T=KCTC 42022T).

Apirhabdus apintestini gen. nov., sp. nov., a member of a novel genus of the family Enterobacteriaceae, isolated from the gut of the western honey bee Apis mellifera

“…The genome of the isolate also encoded systems for flagella and fimbriae construction including transcriptional regulators, biosynthesis/assembly proteins, secretion chaperones, hook, ring, basal-body rod and associated proteins, motor switch proteins filament proteins, stator proteins as well as several genes associated with chemotaxis (Table S3). The discovery of flagellar genes was expected given the observed motility of strain CA-0114 T in semi solid media and motility was demonstrated previously [68] in the close relatives Tenebrionicola larvae YMB-R21 T and Entomohabitans teleogrylli KCTC 42022 T with both possessing genes for flagella biosynthesis and assembly. The genome also contained genes involved in colanic acid production which were absent in the genomes of the relatives Tenebrionicola larvae YMB-R21 T , Tenebrionibacter intestinalis BIT-L3 T , Entomohabitans teleogrylli SCU B244 T and Shimwellia blattae DSM 4481 T .…”

“…Differential biochemical characteristics between strain CA-0114 T and closely related members within the family Enterobacteriaceae Strains: 1, CA-0114 T ; 2, Tenebrionicola larvae YMB-R21 T ; 3, Tenebrionibacter intestinalis BIT-L3 T . Data for Tenebrionicola larvae YMB-R21 T (GCA_019148575.1) was obtained from Lee et al [68]. Data for Tenebrionibacter intestinalis BIT-L3 T (GCA_016632365.1) was obtained from Hu and Yang [67].…”

“…The taxonomic novelty of strain CA-0114 T at the genus level within the Enterobacteriaceae was also confirmed using the GTDB taxonomic classifier version 1.7.0 software [ 65 ] (topology and ANI method) as implemented in the KBase server [ 66 ]. Interestingly, we found that the AAI, OrthoANIu, dDDH and 16S rRNA identity values between Tenebrionibacter intestinalis BIT-L3 T and Tenebrionicola larvae YMB-R21 T were alarmingly high (99.64, 99.83, 98.20 and 99.24 %, respectively) which suggests that these species are subjective synonyms [ 67 68 ].…”