Tenogenic differentiation of human induced pluripotent stem cell-derived mesenchymal stem cells dictated by properties of braided submicron fibrous scaffolds

Czaplewski, Sarah K.; Tsai, Tsung‐Lin; Duenwald-Kuehl, Sarah; Vanderby, Ray; Li, Wan‐Ju

doi:10.1016/j.biomaterials.2014.05.006

Cited by 71 publications

(94 citation statements)

References 31 publications

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“…Considering the importance of crimped fibres to the mechanical properties and biological function of tendons, inclusion of such structures should be taken into account in the design of tendon scaffolds. The biomimicry of crimp structure or its induced mechanical outcome is usually achieved by crimped fibre fabrication or tuning the angles of braided fibre bundles (Czaplewski, Tsai, Duenwald‐Kuehl, Vanderby Jr., & Li, , Surrao, Fan, Waldman, & Amsden, ; Wu, Wu, Vijayavenkataraman, Wong, & Fuh, ).…”

Section: Design Of Tendon Tissue‐engineered Scaffoldsmentioning

confidence: 99%

“…PGA is mechanically stronger than PLA, and the mechanical features of PLGA could be tailored by altering the ratio of glycolic acid and lactic acid (Lu et al, ). Scaffolds fabricated using PCL, PLA, and PGA show that PCL is tougher than PLA (Czaplewski et al, ) and weaker than PGA mechanically (Aghdam et al, ). In terms of degradation, PGA scaffolds have been reported to have a rapid mechanical strength decline 2 to 4 weeks after implantation (Ratner, Hoffman, Schoen, & Lemons, ), which is insufficient for tendon repair.…”

Section: Design Of Tendon Tissue‐engineered Scaffoldsmentioning

confidence: 99%

“…Also, MSCs become scarce with increased age (Diekman et al, ). In addition, human embryonic stem cells (hESCs; Chen et al, ) and induced pluripotent stem cells (iPSCs; Czaplewski et al, ) have also been considered for tendon TE, which were differentiated to MSCs prior to cell seeding. In some studies, human TSPCs and adipose stem cells (ASCs) were also reported to be cell sources for tendon TE (Laranjeira et al, ; Yin et al, ; Yin et al, ).…”

Section: Design Of Tendon Tissue‐engineered Scaffoldsmentioning

confidence: 99%

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Fibre-based scaffolding techniques for tendon tissue engineering

Han

Wong

et al. 2018

J Tissue Eng Regen Med

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Tendon refers to a band of tough, regularly arranged, and connective tissue connecting muscle and bone, transferring strength from muscle to bone, and enabling articular stability and movement. The limitations of natural tendon grafts motivate the scaffold-based tissue engineering (TE) approaches, which aim to build patient-specific biological substitutes that can repair the damaged or diseased tissues. Advances in engineering and knowledge of chemistry and biology have brought forth numerous fibre-based technologies, including electrospinning, electrohydrodynamic jet printing, electrochemical alignment technique, and other fibre-assembly technologies, which enable the fabrication of tendon tissue structure in 3-dimension. Textile techniques such as knitting and braiding have also been performed based on the fibrous materials to produce more complex structure. These scaffolds showed great similarity with native tendons in architectural features, mechanical properties, and facilitate biological functionality such as cellular adhesion, ingrowth, proliferation, and differentiation towards tendon tissue. Herein, we review the techniques that have been used to assemble fibres into scaffolds for tendon TE application. The morphological structures, mechanical properties, materials, degradation characteristics, and biological activities of the induced scaffolds were compared. The existing challenges and future prospects of fibre-based tendon TE have also been discussed.

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Section: Design Of Tendon Tissue‐engineered Scaffoldsmentioning

confidence: 99%

Section: Design Of Tendon Tissue‐engineered Scaffoldsmentioning

confidence: 99%

Section: Design Of Tendon Tissue‐engineered Scaffoldsmentioning

confidence: 99%

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Fibre-based scaffolding techniques for tendon tissue engineering

Han

Wong

et al. 2018

J Tissue Eng Regen Med

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“…MSCs can differentiate into tenocytes to promote tendon healing [2][3][4]. Engineered in vitro nanofiber scaffolds mimic the native topography of the extracellular matrix (ECM) microenvironmental cues that influence cell response and function including adhesion, migration, proliferation, and differentiation [5,6]. Scaffold directed MSC differentiation is determined through a combination of morphology, panels of tendon-related markers, collagen production and alignment, and levels of glycosaminoglycan (GAG) content [7,8].…”

Section: Introductionmentioning

confidence: 99%

Aligned Nanofiber Topography Directs the Tenogenic Differentiation of Mesenchymal Stem Cells

2017

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Abstract:Tendon is commonly injured, heals slowly and poorly, and often suffers re-injury after healing. This is due to failure of tenocytes to effectively remodel tendon after injury to recapitulate normal architecture, resulting in poor mechanical properties. One strategy for improving the outcome is to use nanofiber scaffolds and mesenchymal stem cells (MSCs) to regenerate tendon. Various scaffold parameters are known to influence tenogenesis. We designed suspended and aligned nanofiber scaffolds with the hypothesis that this would promote tenogenesis when seeded with MSCs. Our aligned nanofibers were manufactured using the previously reported non-electrospinning Spinneret-based Tunable Engineered Parameters (STEP) technique. We compared parallel versus perpendicular nanofiber scaffolds with traditional flat monolayers and used cellular morphology, tendon marker gene expression, and collagen and glycosaminoglycan deposition as determinants for tendon differentiation. We report that compared with traditional control monolayers, MSCs grown on nanofibers were morphologically elongated with higher gene expression of tendon marker scleraxis and collagen type I, along with increased production of extracellular matrix components collagen (p = 0.0293) and glycosaminoglycan (p = 0.0038). Further study of MSCs in different topographical environments is needed to elucidate the complex molecular mechanisms involved in stem cell differentiation.

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“…Similarly, synthetic materials such as poly‐ l ‐lactic acid (PLLA), polylactide‐ co ‐glycolide (PLGA), and polycaprolactone (PCL) have been used to produce fibrous meshes. These matrices are ideal for mimicking the structure of connective tissues, as they can be tuned with reference to fiber alignment and diameter, as well as matrix mechanical properties . Current strategies to evaluate these matrices for promotion of tenogenic differentiation of MSCs include optimization of matrix topography, matrix mechanical properties, and ECM components.…”

Section: Cell–matrix Interactionsmentioning

confidence: 99%

Designing the stem cell microenvironment for guided connective tissue regeneration

Bogdanowicz

2017

Annals of the New York Academy of Sciences

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Adult mesenchymal stem cells (MSCs) are an attractive cell source for regenerative medicine because of their ability to self-renew and their capacity for multilineage differentiation and tissue regeneration. For connective tissues, such as ligaments or tendons, MSCs are vital to the modulation of the inflammatory response following acute injury while also interacting with resident fibroblasts to promote cell proliferation and matrix synthesis. To date, MSC injection for connective tissue repair has yielded mixed results in vivo, likely due to a lack of appropriate environmental cues to effectively control MSC response and promote tissue healing instead of scar formation. In healthy tissues, stem cells reside within a complex microenvironment comprising cellular, structural, and signaling cues that collectively maintain stemness and modulate tissue homeostasis. Changes to the microenvironment following injury regulate stem cell differentiation, trophic signaling, and tissue healing. Here, we focus on models of the stem cell microenvironment that are used to elucidate the mechanisms of stem cell regulation and inspire functional approaches to tissue regeneration. Recent studies in this frontier area are highlighted, focusing on how microenvironmental cues modulate MSC response following connective tissue injury and, more importantly, how this unique cell environment can be programmed for stem cell-guided tissue regeneration.

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Tenogenic differentiation of human induced pluripotent stem cell-derived mesenchymal stem cells dictated by properties of braided submicron fibrous scaffolds

Cited by 71 publications

References 31 publications

Fibre-based scaffolding techniques for tendon tissue engineering

Fibre-based scaffolding techniques for tendon tissue engineering

Aligned Nanofiber Topography Directs the Tenogenic Differentiation of Mesenchymal Stem Cells

Designing the stem cell microenvironment for guided connective tissue regeneration

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