Background Traditional treatments for pelvic organ prolapse (POP) assume weak pelvic tissue as the cause. Pelvic floor muscle training has been shown to improve POP symptoms but not prolapse stage and does not reduce rates of referral for future treatment (e.g., pessary or surgery). An alternative hypothesis is proposed that excessive tissue stiffness causes tension and pulling, which disrupts pelvic organ arrangement. Objectives Treating pelvic tissue stiffness through Biotensegrity Focused Therapy is anticipated to improve organ position and prolapse symptoms. Study Design Non-randomised prospective clinical case-series. Methods Women presenting to a private physiotherapy practice in the United Kingdom with POP received Biotensegrity Focused Therapy. Treatment included a series of 1hour physiotherapy sessions focusing on identifying areas of stiff pelvic tissue and using a direct myofascial release technique to normalise elasticity to restore dynamic equilibrium within the pelvis. Prolapse grade (mild, moderate, severe), pelvic floor strength (Modified Oxford Grading Scale), and self-reported symptoms (Pelvic Floor Distress Inventory, PFDI-20) were assessed at baseline and after final treatment. Results 33 women received treatment. At baseline 23/33 (70%) presented with moderate to severe prolapse. After final treatment 3/33 (9%) had moderate and 0/33 (0%) had severe prolapse. 12/33 (36%) achieved complete recovery of organ position and shape. 27/33 (81.82%) women improved by at least one prolapse stage. Pelvic floor strength improved from mean 1.28 (SD 0.85) at baseline to 3.33 (SD 0.78) after final treatment. Self-reported PFDI-20 scores corroborated clinical observations, reducing from mean 98.77 (SD 42.43) at baseline to 49.87 (SD27.28) after final treatment, suggesting a clinically meaningful improvement. Mean treatments received was 6.06 (SD 2.28) sessions over a duration of 4.39 (SD 2.46) months. Discussion Biotensegrity Focused Therapy was shown to have a beneficial impact on organ position and prolapse symptoms and may be an appropriate treatment for women with POP.