Tensile strength, growth factor content and proliferation activities for two platelet concentrates of platelet-rich fibrin and concentrated growth factor

Lee, Hung-Maan; Shen, E-Chin; Shen, John T.; Fu, Earl; Chiu, Hsien‐Chung; Hsia, Yi-Jan

doi:10.1016/j.jds.2020.03.011

Cited by 37 publications

(35 citation statements)

References 34 publications

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“…Previous experiments have revealed that several key growth factors including platelet derived growth factor (PDGF), vascular endothelial growth factor (VEGF), and transforming growth factor beta 1 (TGF β -1) were surely concentrated in PRF, significantly promoting angiogenesis and modulating wound healing process [ [10] , [11] , [12] ]. However, the gelatinous morphology of the PRF lacks stable shape and resistance to compression or stretching because of its strong tensile strength [ [13] , [14] , [15] ], consequently limiting the translational potential of PRF to clinics [ 16 ]. Lyophilization is a commonly used process and has been a widely utilized technique to prepare proteins and platelets [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of lyophilized platelet-rich fibrin in alginate-hyaluronic acid hydrogel as a novel vascularized substitution for myocardial infarction

Qian

Yang

Wang

et al. 2022

Bioactive Materials

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Cardiovascular diseases such as myocardial infarction (MI) are among the major causes of death worldwide. Although intramyocardial injection of hydrogels can effectively enhance the ventricular wall, this approach is limited because of its restriction to the poor vascularization in the infarcted myocardium. Here, we reported a new type of hydrogel composed of alginate (ALG) and hyaluronic acid (HA) with lyophilized platelet-rich fibrin (Ly-PRF) for releasing abundant growth factors to realize their respective functions. The results of in vitro studies demonstrated favorable mechanical property and release ability of ALG-HA with Ly-PRF. When injected into the infarcted myocardium, this composite hydrogel preserved heart function and the Ly-PRF within the hydrogel promoted angiogenesis and increased vascular density in both infarcted and border zone, which rescued the ischemic myocardium. These beneficial effects were also accompanied by macrophage polarization and regulation of myocardial fibrosis. Moreover, the autologous origin of Ly-PRF with ALG-HA hydrogel offers myriad advantages including safety profile, easiness to obtain and cost-effectiveness. Overall, this study demonstrated the versatile therapeutic effects of a novel composite hydrogel ALG-HA with Ly-PRF, which optimizes a promising vascularized substitution strategy for improving cardiac function after MI.

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Section: Introductionmentioning

confidence: 99%

Encapsulation of lyophilized platelet-rich fibrin in alginate-hyaluronic acid hydrogel as a novel vascularized substitution for myocardial infarction

Qian

Yang

Wang

et al. 2022

Bioactive Materials

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“…The modified centrifugation procedure for CGF contributes to a relatively denser fibrin scaffold, which is similar to the natural extracellular matrix (ECM). CGF also contains a higher concentration of growth factors than PRP or PRF, and it exhibits superior regenerative capacity and biomaterial potential [ 2 , 4 , 5 ]. CGF has emerged as a promising biomaterial in tissue remodeling and regeneration, including dermal augmentation and fracture healing, by facilitating epidermal repair, bone formation, nerve repair and angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

Concentrated growth factor regulates the macrophage-mediated immune response

Liu

Zhou

et al. 2021

Regenerative Biomaterials

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Concentrated growth factor (CGF) is a promising regenerative material that serves as a scaffold and adjunct growth factor for tissue engineering. The host immune response, particularly macrophage activity, plays a critical role in injury repair and tissue regeneration. However, the biological effect of CGF on the immune response is not clear. To enrich the theoretical groundwork for clinical application, the present study examined the immunoregulatory role of CGF in macrophage functional activities in vitro. The CGF scaffold appeared as a dense fibrin network with multiple embedded leukocytes and platelets, and it was biocompatible with macrophages. Concentrated bioactive factors in the CGF extract enhanced THP-1 monocyte recruitment and promoted the maturation of suspended monocytes into adherent macrophages. CGF extract also promoted THP-1 macrophage polarization toward the M2 phenotype with upregulated CD163 expression, as detected by cell morphology and surface marker expression. A cytokine antibody array showed that CGF extract exerted a regulatory effect on macrophage functional activities by reducing secretion of the inflammatory factor interleukin (IL)-1β while inducing expression of the chemokine RANTES. Mechanistically, the AKT signaling pathway was activated, and an AKT inhibitor partially suppressed the immunomodulatory effect of CGF. Our findings reveal that CGF induces a favorable immune response mediated by macrophages, which represents a promising strategy for functional tissue regeneration.

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“…The study by Isobe et al found extremely similar composition, mechanical strength, degradation, fibrin fibers thickness and crosslink density of PRF and CGF, in spite of marked differences in the centrifugation process between the two products [21]. The study by Lee et al found higher tensile strength, higher concentration and amount of PDGF-BB and EGF in CGF, as compared to PRF [41]. In addition, osteoblasts proliferation in cultures enriched with PRF or CGF clots at different % (5%, 10%, and 50%) was comparable to cultures with the medium enriched with fetal bovine serum (FBS, 10%).…”

Section: Discussionmentioning

confidence: 97%

“…Recent in vitro studies compared the features and the biological activity of the advanced PRF clot obtained with the original protocol, and the CGF clot obtained with the Medifuge System, manufactured by Silfradent [21,41]. The study by Isobe et al found extremely similar composition, mechanical strength, degradation, fibrin fibers thickness and crosslink density of PRF and CGF, in spite of marked differences in the centrifugation process between the two products [21].…”

Section: Discussionmentioning

confidence: 99%

Concentrated Growth Factors vs. Leukocyte-and-Platelet-Rich Fibrin for Enhancing Postextraction Socket Healing. A Longitudinal Comparative Study

et al. 2020

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Platelet concentrates (PCs) have been used for over 20 years in dentistry, as an adjunct to oral surgery procedures, to improve hard and soft tissue healing and control postoperative symptoms. Among various PCs, Leukocyte and Platelet-Rich Fibrin (L-PRF) has become very popular due to its excellent cost-effectiveness ratio, and to the simple preparation protocol, but comparative clinical studies with other PCs are lacking. The aim of this split-mouth cohort study was to evaluate the effect of Concentrated Growth Factors (CGF), a recently introduced PC, as compared to L-PRF for enhancing post-extraction socket healing. Methods: Patients in need of bilateral tooth extractions were included. Each side was treated with either CGF or L-PRF. Pain, socket closure and healing index were the main outcomes. Results: Forty-five patients (24 women), aged 60.52 ± 11.75 years (range 37–87 years) were treated. No significant difference in outcomes was found, except for Pain at day 1 (p < 0.001) and socket closure in the vestibulo-palatal/lingual dimension at day 7 post-extraction (p = 0.04), both in favor of CGF. Conclusions: based on the present results, CGF proved to be as effective and safe as L-PRF, representing a valid alternative option for improving alveolar socket healing and reducing postoperative discomfort.

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Tensile strength, growth factor content and proliferation activities for two platelet concentrates of platelet-rich fibrin and concentrated growth factor

Cited by 37 publications

References 34 publications

Encapsulation of lyophilized platelet-rich fibrin in alginate-hyaluronic acid hydrogel as a novel vascularized substitution for myocardial infarction

Encapsulation of lyophilized platelet-rich fibrin in alginate-hyaluronic acid hydrogel as a novel vascularized substitution for myocardial infarction

Concentrated growth factor regulates the macrophage-mediated immune response

Concentrated Growth Factors vs. Leukocyte-and-Platelet-Rich Fibrin for Enhancing Postextraction Socket Healing. A Longitudinal Comparative Study

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