Tensiometer-irrigation Control Valve

Peterson, Donald L.; Glenn, D. Michael; Wolford, S. D.

doi:10.13031/2013.25989

Cited by 18 publications

(18 citation statements)

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“…To assess myosin diversity, PCR amplification of myosin-like sequences was performed using the human epithelial cell line Caco-2BBe (16,27). This clonally derived line was selected based on its resemblance to normal human intestinal epithelia (16,24,28). PCR amplification yielded 10 products that bore various degrees of homology to known human and mammalian myosins (Fig.…”

Section: Pcr Analysis Of Myosin Diversity In Human and Porcinementioning

confidence: 99%

Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types.

Bement

Hasson

Wirth

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

152

115

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Myosin divers In the human epi cell line Caco-2Ba, the porcine pihal Cell line LLC PK1 (CL-4)9human peripheral blood leukocytes, and human liver was analyzed. PCR amplification yielded [8][9][10][11] Eukaryotes display a broad array of motility phenomena, ranging from muscle-driven movement to translocation of intracellular organelles. The mechanisms underlying these phenomena are not entirely clear, but it is generally thought that "molecular motors," such as kinesins, dyneins, and myosins, act at cytoskeletal-cytoskeletal and cytoskeletalmembrane interfaces to power organism and cellular movements (1-4). These motors are represented by multiple members in eukaryotes (4, 5), each of which is presumed to play one or more roles during the life of the organism. For example, the fruit fly is known to have at least 6 (6) and possibly as many as 30 (7) kinesins. A unicellular organism, Dictyostelium discoideum, has a minimum offive myosins (8, 9) and probably more than nine (10, 47).Thus, within an organism, multiple diverse molecular motors are the rule. For myosins, phylogenetic analyses have revealed the existence of eight classes (11-13), termed either conventional (filament-forming myosins II) or unconventional (apparently nonfilamentous myosins I and III-VIII) (14). Although evidence indicates that many ofthe unconventional myosin classes will be represented in a given organism (5, 15), to our knowledge, in no case has the number of myosins expressed in a metazoan cell type been determined. Consequently, studies concerning actin-based motility in metazoan cells are complicated by limited information on cellular myosin diversity. We therefore analyzed myosin diversity in two epithelial cell lines, Caco2BBe (16) and LLC-PK1 (17), and in human leukocytes and liver.1 Surprisingly, these cell types express six myosin classes, most of which have at least two representatives. The results challenge the standard "myosin I vs.

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Section: Pcr Analysis Of Myosin Diversity In Human and Porcinementioning

confidence: 99%

Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types.

Bement

Hasson

Wirth

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

152

115

View full text Add to dashboard Cite

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“…Cell Culture-The Caco-2 intestinal epithelial cell line used for this work (Caco-2 BBE ) was a subclone of the original Caco-2 cell line that was selected for its ability to differentiate in culture as indicated by formation of an apical brush border and expression of brush border enzymes (21,22). Passage 45-67 Caco-2 cells were maintained at 37°C with 8% CO 2 in Dulbecco's modified Eagle's medium with 4500 mg/liter D-glucose, 4 mM glutamine, 1 mM sodium pyruvate, 100 units/ml penicillin, 100 g/ml streptomycin, 10 g/ml transferrin, 0.1 mM minimum Eagle's medium nonessential amino acids solution, 10 mM HEPES, pH 7.4, and 3.7 g/liter NaHCO 3 supplemented with 10% fetal bovine serum.…”

Section: Methodsmentioning

confidence: 99%

p130 but Not Paxillin Is Essential for Caco-2 Intestinal Epithelial Cell Spreading and Migration on Collagen IV

Sanders

Basson

2005

Journal of Biological Chemistry

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cas siRNA and the combined Crk/CrkL siRNAs strongly inhibited (52 and 46% inhibition, respectively) Caco-2 sheet migration on collagen IV and noticeably inhibited lamellipodial extension, whereas paxillin siRNA only inhibited migration by 18% and did not noticeably affect lamellipodial extension. These results suggest that Src may regulate Caco-2 migration on collagen IV via both p130 cas and paxillin but that Src phosphorylation of p130 cas is more important for this process.

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“…On the other hand, zero microturbulence was used for the spectrum synthesis calculations throughout this paper. Hydrogen line profiles were computed with the BALMER code (Peterson 1969), modified to include the recent Stark broadening calculations by Stehlé (1994), instead of the Vidal et al (1973) Stark broadening tables.…”

Section: Fundamental Stellar Parametersmentioning

confidence: 99%

Doppler Imaging of stellar magnetic fields

Kochukhov¹,

Piskunov²,

Ilyin

et al. 2002

A&A

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Abstract. We used the new magnetic Doppler Imaging code to reconstruct the magnetic field geometry and surface abundance distributions for the classical magnetic CP star α 2 CVn. High-resolution spectropolarimetric observations in the Stokes I and V parameters were collected with the SOFINéchelle spectrograph at the Nordic Optical Telescope. This superb observational material in combination with the advanced modelling technique allowed to achieve the first simultaneous self-consistent mapping of the vector magnetic field and the abundance distributions of six chemical elements. In recovering the stellar magnetic distribution no prior assumptions about the field geometry or strength were made. Instead, we restricted possible solutions of the inverse problem by means of the multipolar regularization method which searches for the field map close to a general non-axisymmetric multipolar configuration but allows departures from this geometry if that is required by the observational data. We found that the magnetic field of α 2 CVn is dominated by a dipolar component and has a minor quadrupole contribution. The surface distributions of the chemical species form symmetric patterns which closely follow the magnetic geometry. This discovery constitutes one of the first direct observational constraints on the horizontal diffusion processes acting in the upper envelope of a strongly magnetized stellar atmosphere. In addition to the extensive magnetic Doppler Imaging analysis, we derived new accurate estimates of the atmospheric parameters and basic physical properties of α 2 CVn using the energy distribution, hydrogen line profiles and the recent Hipparchos parallax.

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Tensiometer-irrigation Control Valve

Cited by 18 publications

References 0 publications

Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types.

Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types.

p130 but Not Paxillin Is Essential for Caco-2 Intestinal Epithelial Cell Spreading and Migration on Collagen IV

Doppler Imaging of stellar magnetic fields

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