Tentative Identification of Bioactive Compounds From Aqueous Extract of Punica granatum Peel by HPLC/LC–MS Method

Pandit, Bijay Raj; Vyas, Ashish

doi:10.1007/s40011-021-01294-3

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…As water is a highly polar solvent due to the presence of a hydroxyl group and oxygen molecule while ethanol is less polar. These properties enable the hydroethanolic solvent for maximum extraction yield as compared with the pure solvent as discussed by (Pandit & Vyas, 2021).…”

Section: Yield Of Extractmentioning

confidence: 99%

Phytochemical characterisation and antioxidant capacities of pomegranate peel

Kafeel

Inam‐Ur‐Raheem

Khan

et al. 2023

Int J of Food Sci Tech

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SummaryPomegranate peel, a biological waste, is of great interest due to its higher gallic acid, catechin and quercetin contents and their therapeutic action. The current research focuses on the evaluation of pomegranate peel extracts of two cultivars, Badana and Kandhari. Polyphenols from various concentrations of ethanol were extracted and analysed by modern techniques. Results indicated that the extract of Badana showed the highest phenolics (667.14 ± 7.48 mg GAE/g), flavonoids (151.54 ± 2.01 mg CE/g), antioxidant activity by DPPH (87.77% ± 0.16% inhibition) and FRAP (70.54 ± 0.25 mmol/100 g). Also, the 70% hydroalcoholic Kandhari extract exhibited the highest flavonoids in comparison to 50% and 100% extracts. Fourier Transform Infrared spectroscopy spectra indicated numerous functional groups such as alkyl halides, alcohols, polyphenols, alkenes, carbonyl and carboxylic acid. The HPLC chromatogram revealed the presence of gallic acid, quercetin, ferulic acid, benzoic acid, caffeic acid, cinnamic acid, vanillic acid, syringic acid and sinapic acid with the highest concentration of gallic acid in Badana extract. So, here we concluded that 70% hydroethanolic Badana extract is an excellent source of bioactive components and can further be utilised for value addition and curative attributes.

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Section: Yield Of Extractmentioning

confidence: 99%

Phytochemical characterisation and antioxidant capacities of pomegranate peel

Kafeel

Inam‐Ur‐Raheem

Khan

et al. 2023

Int J of Food Sci Tech

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Punica granatum L. polysaccharides: A review on extraction, structural characteristics and bioactivities

Zhao,

Zhang,

Guo

et al. 2024

Carbohydrate Research

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LC–ESI–MS phenolic contents assessment, antioxidant, and protective ability of Punica granatum root bark extract against ethanol-induced gastric ulcer in rats: in silico H+, K+-ATPase inhibitory pathway study

Alimi

Mabrouk

Zouari

et al. 2023

Toxicology Research

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The objectives of the current study were to evaluate the Punica granatum root bark extract’s (PGE) antioxidant and gastroprotective activities against ethanol-induced gastric ulcers in Wistar rats and to elucidate the putative mechanism of action using in silico analysis. The PGE phytochemical study shows high levels of phenolics, flavonoids, tannins, and polysaccharides. In vitro, the PGE was more effective at scavenging hydroxyl radicals than quercetin and had lower ferric reducing activity than catechin. In vivo, it was revealed that pretreatment of ethanol-ulcerated rats with PGE at oral doses of 100, 200, and 400 mg/kg b.w. offered a dose-dependent shield against ethanol-induced ulcers when compared to Omeprazole (20 mg/kg b.w.) by preventing the development of deep ulcer lesions, lowering gastric juice output and pH rises, boosting gastric mucus production and antioxidant enzyme levels, and attenuating malondialdehyde and myeloperoxidase contents. Moreover, the liquid chromatography–mass spectrometry analysis of PGE identified 5 phenolic acids and 4 flavonoids, which revealed an in silico high oral bioavailability, drug-likenesses, and good binding affinities and thus inhibitory effects on the gastric H+, K+-ATPase enzyme. PGE may have synergistic antioxidant, anti-inflammatory, and H+, K+-proton pump inhibitory actions that contribute to its antiulcer efficacy.

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Tentative Identification of Bioactive Compounds From Aqueous Extract of Punica granatum Peel by HPLC/LC–MS Method

Cited by 3 publications

References 24 publications

Phytochemical characterisation and antioxidant capacities of pomegranate peel

Phytochemical characterisation and antioxidant capacities of pomegranate peel

Punica granatum L. polysaccharides: A review on extraction, structural characteristics and bioactivities

LC–ESI–MS phenolic contents assessment, antioxidant, and protective ability of Punica granatum root bark extract against ethanol-induced gastric ulcer in rats: in silico H+, K+-ATPase inhibitory pathway study

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