Tentative Inhibition Zone Criteria (Bauer-Kirby Agar Disk Diffusion Method) for Rifampin against Staphylococci

Traub, Walter H.; Spohr, Marlene; Bauer, Dierk

doi:10.1159/000238529

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…The zone of inhibition around the discs were measured in millimeter (mm) and expressed as mean±SE. Criteria for resistance, susceptibility and intermediate resistance or susceptible were followed according to the Traub et al 18) . Diameter of inhibition zones less than or equal to 13 mm＝resistant; diameter 14-21 mm＝intermediate susceptible; diameter greater than or equal to 22 mm ＝susceptible were considered.…”

Section: Antibiogram Studymentioning

confidence: 99%

Honey Potential as Antibiofilm, Antiquorum Sensing and Dispersal Agent against Multispecies Bacterial Biofilm

Liaqat¹,

Shahzad²,

Hanif

et al. 2021

J. Oleo Sci.

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biofilm is quite complicated because of the mechanisms underlying bacterial growth as well as resistance. Multispecies biofilms have complementary metabolic strategies for obtaining nutrients and degrading host immune molecules 3) . Various strategies have been developed and implicated for the biofilm control, however, natural and ef-Abstract: This study is first to test Pakistani honey bees, Apis dorsata and A. cerana honey samples as anti biofilm, anti quorum sensing (QS) and biofilm dispersal agents honey against multispecies biofilm of bacteria (obtained from obese patients). Briefly, five previously identified isolates Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Morganella morganii and Klebsiella pneumoniae (MT448672-MT448676) were selected. Antibiogram study of all five isolates was tested against three antibiotics viz., erythromycin (20 µg/mL), lincomycin (100 µg/mL) and rifampicin (100 µg/mL). In order to form multispecies biofilm, identified bacteria were grown in batch culture by mixing equal volumes (OD 590nm = 0.1) of 2, 3 and 5 bacterial isolates. In total 11 groups (g1-g11) were made. Crystal violet (CV) staining method was used to evaluate the antibiofilm potential and biofilm dispersal potential of both honey samples. QS inhibition in P. aeruginosa was measured following culture supernatant method. Antibiogram study showed significant (p < 0.05) resistance by P. aeruginosa against tested antibiotics. E. coli, M. morganii and K. pneumoniae were significantly susceptible to erythromycin and S. aureus to lincomycin. Both honey samples at 2% and 5% concentrations showed significant (p < 0.05) inhibition potential of multispecies biofilm by all test groups (g1-g11). Though A. dorsata honey significantly inhibited biofilm formation at 2 and 5% against all groups but 2% concentration was highly significant against g2-g4 groups. Regarding A. cerana honey, 2% concentration was significantly effective against g1, g4-g7 and g9-g11 groups. Both honey samples significantly inhibited QS at 2 and 5%. The 5% concentration of A. dorsata honey significantly dispersed biofilm by all groups compared to 2% which showed dispersal potential only by g2 and g3 groups. Accordingly, honey samples showed significant antibiofilm, anti-QS and biofilm dispersal potentials thus can be considered as good alternative to antibiotics.

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Section: Antibiogram Studymentioning

confidence: 99%

Honey Potential as Antibiofilm, Antiquorum Sensing and Dispersal Agent against Multispecies Bacterial Biofilm

Liaqat¹,

Shahzad²,

Hanif

et al. 2021

J. Oleo Sci.

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“…Nitrocefin (Glaxo Group Research Ltd., Greenford, Middx, UK) served for detection of (i-lactamase production (5). High-content antibiotic disks, including 10 pg imipenem and 5 ug novobiocin disks, were procured from Becton Dickinson GmbH (Heidelberg, FRG); 2 pg rifampin disks [11] were purchased from Oxoid Deutschland GmbH (Wesel, FRG).…”

Section: Methodsmentioning

confidence: 99%

<i>Staphylococcus haemolyticus</i>: Cocarde Growth around Imipenem Disks

Traub

Kohl²,

Spohr³

1988

Chemotherapy

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“…The antibacterial activity of colored-rice extracts was examined using several reliable techniques. It has been noted that Bauer-Kirby disk susceptibility-, MIC-and MBC-based determination are widely used for investigating the antimicrobial susceptibility of various bacteria (Gonzalez et al, 2013;Liberman & Robertson, 1975;Traub, Spohr, & Bauer, 1987;Van Asselt & Van Boven, 1997).…”

Section: Assessment Of Bacterial Survivalmentioning

confidence: 99%

The In-vitro Antibacterial Effect of Colored Rice Crude Extracts against Staphylococcus aureus Associated with Skin and Soft-Tissue Infection

Pumirat¹,

Luplertlop²

2013

JAS

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Due to increases in the resistance of bacteria to the existing antimicrobial agents, plants are being used as alternative sources for the development of safe, effective, and inexpensive new agents to treat and prevent bacterial infections. Recent studies have shown that rice (Oryza sativa L.), an important source of nutrients, consumed by most of the world's population, can suppress some bacterial infections. There are many varieties of rice, e.g. white, brown, black, and red; however, the relationship between rice color and antibacterial properties remains unclear. In the present study, we investigated the antibacterial activity of colored-rice crude extracts from four different types of colored rice (Hom Nil, Neaw Dum, Mun Poo, and Sang Yod) against common bacteria causing skin and soft-tissue infections, such as Staphylococcus aureus, Streptococcus pyogenes, Enterococcus spp., Pseudomonas aeruginosa, and Escherichia coli. The results showed that all colored-rice crude extracts had antibacterial effects against S. aureus; and more notably, crude extracts of differently colored rice restricted diverse antibacterial activities. To our knowledge, this is the first report that provides a basic understanding of the antibacterial properties of colored rice against skin and wound pathogens. An understanding of these properties would be invaluable in the development of alternative, natural, and safe methods of controlling bacterial infections.

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Tentative Inhibition Zone Criteria (Bauer-Kirby Agar Disk Diffusion Method) for Rifampin against Staphylococci

Cited by 6 publications

References 22 publications

Honey Potential as Antibiofilm, Antiquorum Sensing and Dispersal Agent against Multispecies Bacterial Biofilm

Honey Potential as Antibiofilm, Antiquorum Sensing and Dispersal Agent against Multispecies Bacterial Biofilm

<i>Staphylococcus haemolyticus</i>: Cocarde Growth around Imipenem Disks

The In-vitro Antibacterial Effect of Colored Rice Crude Extracts against Staphylococcus aureus Associated with Skin and Soft-Tissue Infection

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