Tenuifilum thalassicum gen. nov., sp. nov., a novel moderate thermophilic anaerobic bacterium from a Kunashir Island shallow hot spring representing a new family Tenuifilaceae fam. nov. in the class Bacteroidia

Podosokorskaya, Olga A.; Kochetkova, Tatiana V.; Новиков, А. А.; Toshchakov, Stepan V.; Elcheninov, Alexander G.; Kublanov, Ilya V.

doi:10.1016/j.syapm.2020.126126

Cited by 23 publications

(3 citation statements)

References 63 publications

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“…Individual correlations of the most abundant taxa with elemental composition, assessed by Spearman’s rank-order correlation analysis, showed that 14 genera significantly ( p -Value < 0.05) correlated with element content in the environment. Thus, Thiofaba and Tenuifilum [ 51 ] correlated with sulfur and magnesium, as was also supported by CCA analysis. Interestingly, chemolitoautotrophic sulfur-oxidizing Thiobacter sp., abundant in the Karmadon springs, did not show correlation with sulfur, but strongly correlated with iron ( p < 0.01; Figure 4 C).…”

Section: Resultssupporting

confidence: 61%

Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus

Toshchakov

Izotova

Vinogradova

et al. 2021

Biology

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The Greater Caucasus is a part of seismically active Alpine–Himalayan orogenic belt and has been a center of significant volcanic activity during the Quaternary period. That led to the formation of the number of hydrothermal habitats, including subterranean thermal aquifers and surface hot springs. However, there are only a limited number of scientific works reporting on the microbial communities of these habitats. Moreover, all these reports concern only studies of specific microbial taxa, carried out using classical cultivation approaches. In this work, we present first culture-independent study of hydrotherms in the Republic of North Ossetia-Alania, located in the southern part of the North Caucasus. Using 16S metabarcoding, we analyzed the composition of the microbial communities of two subterranean thermal aquifers and terrestrial hot springs of the Karmadon valley. Analysis of correlations between the chemical composition of water and the representation of key taxa allowed us to identify the key factors determining the formation of microbial communities. In addition, we were able to identify a significant number of highly abundant deep phylogenetic lineages. Our study represents a first glance on the thermophilic microbial communities of the North Caucasus and may serve as a basis for further microbiological studies of the extreme habitats of this region.

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Section: Resultssupporting

confidence: 61%

Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus

Toshchakov

Izotova

Vinogradova

et al. 2021

Biology

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“…Some studies have used Proteobacteria and Acidobacteria as indicators of land use change and soil pollution ( Schneider et al., 2017 ; Kim et al., 2021 ). Members of the class Bacteroidia are Gram-negative, non-spore-forming, mostly anaerobic bacteria that decompose organic substances, and they thrive in diverse environments, such as soils and subsurface sediments ( Garcia-Lopez et al., 2019 ; Podosokorskaya et al., 2020 ). Ascomycota is by far the largest group in the fungal kingdom.…”

Section: Resultsmentioning

confidence: 99%

Safety evaluation of FAD2 RNAi transgenic Brassica napus L. based on microbial diversity and metabonomic analysis

Wang

Xie

et al. 2022

Front. Plant Sci.

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Oleic acid desaturase (FAD2) is the key enzyme that produces polyunsaturated fatty acids in rapeseed (Brassica napus L), which is one of the main oil crops. RNA interference (RNAi) is an emerging technique that provides new opportunities for the generation of new traits in plants. To increase oleic acid content and reduce linoleic and linolenic acid content in rapeseed, we constructed an ihpRNA plant expression vector of the FAD2 gene and obtained transgenic plants for multiple generations by stable inheritance. In this study, third-generation transgenic plants (T3), seventh-generation transgenic plants (T7), and wild-type plants (WT) were used. The differences in microbial community diversity between transgenic plants and wild-type plants and the up- and downregulation of rhizosphere metabolite contents were investigated. In conclusion, the results showed that the soil microbial community structure was stable, the general microbial community structure was not changed by the transgenic rhizosphere exudates, and no significant harmful root exudate of transgenic rapeseed on the environment was found through the microbial community and metabolomics analysis. This work may provide an understanding of the impact of RNAi on plant metabolites and a safety evaluation method for transgenic plants and a reference for rapeseed breeding.

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“…, in the HL group, shown in Figure 5A ). Nonetheless, significant decreases in the abundance of Bacteroidia (Bacteroidetes) were found in the HL group ( Figure 5B ), possibly due to Bacteroidia being chemo-organoheterotrophs with growth on carbohydrates or peptide mixtures and proteins ( Podosokorskaya et al, 2020 ). Therefore, the dominant taxa presented different ecotypes, nutritional preferences, and responses to nutrient fluctuations.…”

Section: Discussionmentioning

confidence: 99%

Comparison and interpretation of freshwater bacterial structure and interactions with organic to nutrient imbalances in restored wetlands

et al. 2022

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Chemical oxygen demand to nitrogen (COD/N) and nitrogen to phosphorus (N/P) ratios have distinct effects on bacterial community structure and interactions. However, how organic to nutrient imbalances affect the structure of freshwater bacterial assemblages in restored wetlands remains poorly understood. Here, the composition and dominant taxa of bacterial assemblages in four wetlands [low COD/N and high N/P (LH), low COD/N and low N/P (LL), high COD/N and high N/P (HH), and high COD/N and low N/P (HL)] were investigated. A total of 7,709 operational taxonomic units were identified by high throughput sequencing, and Actinobacteria, Proteobacteria, and Cyanobacteria were the most abundant phyla in the restored wetlands. High COD/N significantly increased bacterial diversity and was negatively correlated with N/P (R2 = 0.128; p = 0.039), and the observed richness (Sobs) indices ranged from 860.77 to 1314.66. The corresponding Chao1 and phylogenetic diversity (PD) values ranged from 1533.42 to 2524.56 and 127.95 to 184.63. Bacterial beta diversity was negatively related to COD/N (R2 = 0.258; p < 0.001). The distribution of bacterial assemblages was mostly driven by variations in ammonia nitrogen (NH4+-N, p < 0.01) and electrical conductivity (EC, p < 0.01), which collectively explained more than 80% of the variation in bacterial assemblages. However, the dominant taxa Proteobacteria, Firmicutes, Cyanobacteria, Bacteroidetes, Verrucomicrobia, Planctomycetes, Chloroflexi, and Deinococcus-Thermus were obviously affected by variation in COD/N and N/P (p < 0.05). The highest node and edge numbers and average degree were observed in the LH group. The co-occurrence networkindicated that LH promoted bacterial network compactness and bacterial interaction consolidation. The relationships between organic to nutrient imbalances and bacterial assemblages may provide a theoretical basis for the empirical management of wetland ecosystems.

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Tenuifilum thalassicum gen. nov., sp. nov., a novel moderate thermophilic anaerobic bacterium from a Kunashir Island shallow hot spring representing a new family Tenuifilaceae fam. nov. in the class Bacteroidia

Cited by 23 publications

References 63 publications

Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus

Culture-Independent Survey of Thermophilic Microbial Communities of the North Caucasus

Safety evaluation of FAD2 RNAi transgenic Brassica napus L. based on microbial diversity and metabonomic analysis

Comparison and interpretation of freshwater bacterial structure and interactions with organic to nutrient imbalances in restored wetlands

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