Teosinte ligule allele narrows plant architecture and enhances high-density maize yields

Tian, Jinge; Wang, Chenglong; Xia, Jinliang; Wu, Lijuan; Xu, Guiyun; Wu, Weihao; Ли, Дан; Qin, Wenchao; Han, Xu; Chen, Qiuyue; Jin, Weiwei; Tian, Feng

doi:10.1126/science.aax5482

Cited by 330 publications

(247 citation statements)

References 31 publications

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“…In rice, an increase in methyl jasmonate was found to decrease leaf angle by inhibiting brassinosteroid (BR) biosynthesis, the BR signalling pathway, and BR-induced gene expression (Gan et al, 2015). Recently, Tian et al (2019) dissected a plant architecture QTL that regulates BR and leaf angle, increasing yield in dense population environments, and demonstrated the involvement of LG1 in the regulation of genes controlling these traits. The sympathy for the ligule gene, a modifier of the liguleless narrow1 gene, was also shown to have a pleiotropic role in leaf architecture and disease resistance (Anderson et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Maize nested introgression library provides evidence for the involvement ofliguleless1in resistance to northern leaf blight

Kolkman

Strable

Harline

et al. 2019

Preprint

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25Plant disease resistance is largely governed by complex genetic architecture. In maize, few 26 disease resistance loci have been characterized. Near-isogenic lines (NILs) are a powerful 27 genetic tool to dissect quantitative trait loci (QTL). We analyzed an introgression library of 28 maize near-isogenic lines (NILs), termed a nested NIL (nNIL) library for resistance to northern 29 leaf blight (NLB) caused by the fungal pathogen Setosphaeria turcica. The nNIL library was 30 comprised of 412 BC5F4 NILs that originated from 18 diverse donor parents and a common 31 recurrent parent, B73. Single nucleotide polymorphisms identified through genotyping by 32 sequencing (GBS) were used to define introgressions and for association analysis. NILs that 33 conferred resistance and susceptibility to NLB were comprised of introgressions that overlapped 34 known NLB QTL. Genome-wide association analysis and stepwise regression further resolved 35 five QTL regions, and implicated several candidate genes, including Liguleless1 (Lg1), a key 36 determinant of leaf architecture in cereals. Two independently-derived mutant alleles of lg1 37 inoculated with S. turcica showed enhanced susceptibility to NLB. In the maize nested 38 association mapping population, leaf angle was positively correlated with NLB in five 39 recombinant inbred line (RIL) populations, and negatively correlated with NLB in four RIL 40 populations. This study demonstrates the power of a nNIL library combined with high density 41 SNP coverage to resolve QTLs. Furthermore, the role of lg1 in leaf architecture and in resistance 42 to NLB has important applications in crop improvement.43 44Significance Statement (120 words) 45 Understanding the genetic basis of disease resistance is important for crop improvement. We 46 analyzed response to northern leaf blight (NLB) in a maize population consisting of 412 near-47 isogenic lines (NILs) derived from 18 diverse donor parents backcrossed to a recurrent parent, 48 B73. NILs were genotyped by sequencing to detect introgressed segments. We identified NILs 49 with greater resistance or susceptibility to NLB than B73. Genome-wide association analysis, 50 coupled with stepwise regression, identified 5 candidate loci for NLB resistance, including the 51 liguleless1 gene. The LIGULELESS1 transcription factor is critical in development of the leaf 52 ligular region and influences leaf angle. We found that liguleless1 mutants are significantly more 53 susceptible to NLB, uncovering a pleiotropic role for liguleless1 in development and disease 54 resistance. 55 56 57 58

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Section: Discussionmentioning

confidence: 99%

Maize nested introgression library provides evidence for the involvement ofliguleless1in resistance to northern leaf blight

Kolkman

Strable

Harline

et al. 2019

Preprint

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“…MODEM (http://modem.hzau.edu.cn/) is the first attempt to integrate multi-omics datasets, including various types of genetic variants, expression data and metabolomic data (22). Despite the importance of wild relatives in understanding the domestication of modern crops, no existing maize multi-omics databases incorporate teosinte (23). To fill this gap, we have developed ZEAMAP (http://www.zeamap.com/), a multi-omics database for maize research and breeding, which integrates omics data generated from 527 elite inbred lines (in an association mapping panel, AMP) and 183 teosinte accessions (24).…”

Section: Introductionmentioning

confidence: 99%

ZEAMAP, a comprehensive database adapted to the maize multi-omics era

Gui

Yang

et al. 2020

Preprint

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As one of the most extensively cultivated crops, maize (Zea mays L.) has been extensively studied by researchers and breeders for over a century. With advances in high-throughput detection of various omics data, a wealth of multi-dimensional and multi-omics information has been accumulated for maize and its wild relative, teosinte. Integration of this information has the potential to accelerate genetic research and generate improvements in maize agronomic traits. To this end, we constructed ZEAMAP (http://www.zeamap.com), a comprehensive database incorporating multiple reference genomes, annotations, comparative genomics, transcriptomes, open chromatin regions, chromatin interactions, high-quality genetic variants, phenotypes, metabolomics, genetic maps, genetic mapping loci, population structures and domestication selection signals between teosinte and maize. ZEAMAP is user-friendly, with the ability to interactively integrate, visualize and cross-reference multiple different omics datasets. and linkage analysis, the population structure and pedigrees of each germplasm and the selective signals between different teosinte subspecies and maize. ZEAMAP generated comprehensive functional annotations for the annotated gene models in each assembly, and provided useful tools for users to search, analyze and visualize all these different omics data.

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“…Among the factors involved in determining plant architecture, BR shows great potential in improving this trait for its remarkable function in rice leaf angle regulation and grain size control (Tong and Chu, 2018). An erect leaf angle is beneficial for a higher plant density and more light capture for photosynthesis (Tian et al, 2019), which would thus result in a grain yield increase (Sakamoto et al, 2006). To date, studies on BR have exposed the double faced function of the phytohormone in plant development, i.e., compact stature brought by BR deficiency is always accompanied by small grain size, such as d11 , d2 , brd1 , dlt , d61-1 and d61-2 (Yamamuro et al, 2000; Mori et al, 2002; Hong et al, 2003; Tanabe et al, 2005; Tong et al, 2009), while an enlarged grain size resulting from an excessive amount of BR often develops along with a loose stature, such as that observed for OsBZR1 -OE, GSK2 -RNAi, and D11 -OE transgenic plants (Tong et al, 2012; Zhu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Separable regulation of POW1 in TAF2-mediated grain development and BR-mediated leaf angle formation in rice

Zhang

Wang

et al. 2019

Preprint

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Leaf angle is one of the key factors determining rice plant architecture. However, improvement of the leaf angle appears to be unsuccessful in practical breeding because of the simultaneous occurrence of unfavorable traits such as grain size reduction. In this study, we identified the pow1 (put on weight 1) mutant with enlarged grain size and leaf angle, typical brassinosteroid (BR)-related phenotypes caused by excessive cell proliferation and cell expansion. We show that POW1 encodes a novel protein functioning in grain size regulation by repressing the transcription activity of the interacting protein TAF2, a highly conserved member of the transcription initiation complex TFIID. Loss of function of POW1 increases the phosphorylation of OsBZR1 and decreases the inhibitory effect of OsBZR1 on the transcription of BR biosynthesis genes OsDWARF4 (D4) and D11, thus participates in BR-mediated leaf angle regulation. The separable functions of POW1 in grain size and leaf angle control provide a promising strategy to design high-yielding varieties in which both traits would be favorably developed, i.e., compact plant architecture and increased grain size, thus would promote the high-yield breeding a step forward in rice.

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Teosinte ligule allele narrows plant architecture and enhances high-density maize yields

Cited by 330 publications

References 31 publications

Maize nested introgression library provides evidence for the involvement ofliguleless1in resistance to northern leaf blight

Maize nested introgression library provides evidence for the involvement ofliguleless1in resistance to northern leaf blight

ZEAMAP, a comprehensive database adapted to the maize multi-omics era

Separable regulation of POW1 in TAF2-mediated grain development and BR-mediated leaf angle formation in rice

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