Tephritid fruit flies have a large diversity of co-occurring RNA viruses

R., Sharpe, Stephen; Morrow, Jennifer L.; Brettell, Laura E.; Shearman, Deborah; Gilchrist, Stuart; Cook, James M.; Riegler, Markus

doi:10.1016/j.jip.2021.107569

Cited by 19 publications

(42 citation statements)

References 59 publications

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“…Recently, the wide application of high throughput sequencing has provided a continuous flow of newly discovered ISVs, specifically RNA viruses [ 3 , 4 , 5 ]. Those ISVs belong to diverse families of single and double strand RNA viruses, including Iflaviridae , Nodaviridae , Negeviridae , Togaviridae , Rhabdoviridae , Reoviridae , or Totiviridae [ 2 , 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, up to nine RNA viruses have been identified in C. capitata and all of them seem to be present in a covert state, not causing clear symptoms [ 7 , 8 , 10 , 14 ]. One of these viruses has a negative single-stranded (ss) RNA genome, Ceratitis capitata sigmavirus (CcaSV), which belongs to the Rhabdoviridae family [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies

Hernández-Pelegrín

Llopis-Giménez

Crava

et al. 2022

Viruses

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The Mediterranean fruit fly (medfly), Ceratitis capitata, is an agricultural pest of a wide range of fruits. The advent of high-throughput sequencing has boosted the discovery of RNA viruses infecting insects. In this article, we aim to characterize the RNA virome and viral sRNA profile of medfly. By means of transcriptome mining, we expanded the medfly RNA virome to 13 viruses, including two novel positive ssRNA viruses and the first two novel dsRNA viruses reported for medfly. Our analysis across multiple laboratory-reared and field-collected medfly samples showed the presence of a core RNA virome comprised of Ceratitis capitata iflavirus 2 and Ceratitis capitata negev-like virus 1. Furthermore, field-collected flies showed a higher viral diversity in comparison to the laboratory-reared flies. Based on the small RNA sequencing, we detected small interfering RNAs mapping to all the viruses present in each sample, except for Ceratitis capitata nora virus. Although the identified RNA viruses do not cause obvious symptoms in medflies, the outcome of their interaction may still influence the medfly’s fitness and ecology, becoming either a risk or an opportunity for mass-rearing and SIT applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies

Hernández-Pelegrín

Llopis-Giménez

Crava

et al. 2022

Viruses

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“…MN745080) [ 33 ] and B. tryoni rhabdovirus 1 strain d of a tephritid fruit fly (accession no. MW208811) [ 34 ]. The viruses have been reported as new sigmaviruses in the family Rhabdoviridae [ 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…MW208811) [ 34 ]. The viruses have been reported as new sigmaviruses in the family Rhabdoviridae [ 33 , 34 ]. Sigmavirus genera infects a variety of fruit flies, including Drosophila melanogaster , D. immigrans , and D. ananassae [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

A new cluster of rhabdovirus detected in field-caught sand flies (Diptera: Psychodidae: Phlebotominae) collected from southern Thailand

et al. 2021

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Background The distribution of phlebotomine sand flies is changing rapidly due to climate change. This issue has implications for the epidemiology of sand fly-borne diseases, especially sand fly-associated viruses. Few studies concerning sand fly-associated viruses have been conducted in Thailand. Therefore, this study aimed to perform a molecular survey of groups of pathogenic RNA viruses belonging to the Orbivirus, Phlebovirus, and Flavivirus genera and family Rhabdoviridae in sand fly samples collected from southern Thailand. Methods Sand flies were collected at two locations in Trang and Songkhla provinces of southern Thailand, and individual sand fly samples were processed for species identification and virus detection. The Orbivirus, Phlebovirus, and Flavivirus genera and family Rhabdoviridae molecular determination was performed by RT-PCR, and positive samples were identified by cloning and sequencing, cell culture inoculation, and phylogenetic analysis. Results The results presented in this study were based on the analysis of a total of 331 female sand flies. This molecular study revealed evidence of Rhabdoviridae family virus presence in Phlebotomus papatasi (3/331, 0.9%). The findings demonstrated a new cluster of rhabdovirus that was closely related to Bactrocera dorsalis sigmavirus strain BDSV.abc5 and the lineages of insect-specific Rhabdoviridae. In addition, the Bayesian tree suggested that the common ancestor of this group was the dimarhabdovirus clade. It was assumed that the virus may have switched hosts during its evolution. However, the detection of Orbivirus, Phlebovirus, and Flavivirus genera using specific primers for RT-PCR was negative in the collected sand flies. Conclusions There is limited knowledge on the genetic diversity and ecology of Rhabdoviridae in Thailand. This is the first data regarding the circulation of Rhabdoviridae in Ph. papatasi from Thailand. We found a new cluster of rhabdoviruses that was close to the new B. dorsalis sigmavirus. It is possible that there is a great deal of diversity in this family yet to be discovered, and a more extensive survey for new rhabdoviruses may uncover viruses from a wide diversity of host taxa and broaden our understanding of the relationships among the Rhabdoviridae. Graphical abstract

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“…A portion of the polyprotein shares between ~30% and ~40% pairwise amino acid identity to polyproteins of known iflaviruses and 45% identity to the recently discovered Bactrocera tryoni iflavirus 1 virus assembled from a metagenomic analysis of Bactrocera tryoni (Diptera: Tephritidae) (Sharpe et al 2021). Variant analysis on the sample from which the representative genome was derived uncovered 5 SNPs over the ~10.7 Kbp sequence, which were all synonymous, probably representing a small intra-individual variability.…”

Section: Iflavirus Discovery In G Morsitansmentioning

confidence: 99%

Tsetse RNA Virome: Novel Iflavirus Genomes inGlossina morsitansand Other Tsetse Species

Manni

Zdobnov

2021

Preprint

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Tsetse flies (Glossina spp.) are vectors of Human and Animal African trypanosomiasis. The tsetse microbiome has been extensively studied in the context of bacterial endosymbiont-host interactions, however, remarkably little is known about the tsetse virome with only one well-characterized DNA virus, the salivary gland hypertrophy virus (SGHV). Here we report the genomes of four iflaviruses identified in tsetse flies and their distribution in public RNA-seq libraries, mainly from laboratory colonies. Strikingly, the iflavirus identified in G. morsitans, provisionally named Glossina iflavirus 1 (GliflaV1), is present in all 136 RNA-seq libraries of G. morsitans maintained at different institutions, and displays a broad tissue tropism and high abundance, reaching up to 15% of library content. Its ubiquitous distribution and presence in the reproductive tissues, intrauterine larvae, and teneral flies suggest it is part of the initial core microbiota maternally transmitted to the progeny. None of the G. morsitans samples harbor iflaviruses identified in the other three, more closely related, tsetse species which, conversely, do not harbor the iflavirus from G. morsitans. Though apparently asymptomatic, these infections may influence tsetse host fitness, developmental or biological processes which might be relevant in the context of tsetse population control strategies, mass rearing, and paratransgenesis, and open up new opportunities to study the quadripartite system of interactions among the invertebrate host, the parasitic protozoan, and both viral and bacterial symbionts.

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Tephritid fruit flies have a large diversity of co-occurring RNA viruses

Cited by 19 publications

References 59 publications

Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies

Expanding the Medfly Virome: Viral Diversity, Prevalence, and sRNA Profiling in Mass-Reared and Field-Derived Medflies

A new cluster of rhabdovirus detected in field-caught sand flies (Diptera: Psychodidae: Phlebotominae) collected from southern Thailand

Tsetse RNA Virome: Novel Iflavirus Genomes inGlossina morsitansand Other Tsetse Species

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