Tepidibaculum saccharolyticum gen. nov., sp. nov. a moderately thermophilic, anaerobic, spore-forming bacterium isolated from a terrestrial hot spring

Abstract

A novel moderately thermophilic, anaerobic bacterium, strain STR9 was isolated from terrestrial hot spring at Uzon Caldera, Kamchatka peninsula, Russia. Cells of strain STR9 were spore-forming motile straight or slightly curved rods 0.3 μm in diameter and 1.0-2.0 μm in length having a Gram-positive cell-wall structure. The temperature range for growth was 30-58 °C, with an optimum at 50 °C. The pH range for growth was 5.0-10.5, with an optimum at pH 8.0-9.0. NaCl inhibited growth of the strain STR9 and no growth was evident above 0.5% NaCl (w/v). Several mono-, di-, and polysaccharides as well as beef extract and yeast extract supported growth. The main end products of glucose fermentation were ethanol, acetate, hydrogen, and carbon dioxide. The G + C content of DNA of strain STR9 was 40.0 mol% (T). Analysis of the 16S rRNA gene sequences revealed that strain STR9 belongs to the family Ruminococcaceae, class Clostridia, with less than 93% 16S rRNA gene sequence similarity to any species with validly published name. We propose to assign strain STR9 to a new species of a novel genus Tepidibaculum saccharolyticum gen. nov., sp. nov. The type strain is STR9 (= DSM 28577 = VKM B-2882 = UNIQEM STR9).

Coprobacter secundus subsp. similis subsp. nov. and Solibaculum mannosilyticum gen. nov., sp. nov., isolated from human feces

“…Strains: 1, 12CBH8 T (data from this study); 2, Caproiciproducens galactitolivorans BS‐1 T 35 ; 3, Clostridium methylpentosum DSM 5476 T 36 ; 4, Tepidibaculum saccharolyticum STR9 T 37 ; 5, Ruminococcus bromii ATCC 27255 T 38 . +, positive; −, negative; NA, no data available.…”

Gut Bacteria Shared by Children and Their Mothers Associate with Developmental Level and Social Deficits in Autism Spectrum Disorder

“…This study suggests that butyrate may alleviate the severity of neurodevelopmental delay in children. Many members of the family Ruminococcaceae produce acetate (33)(34)(35)(36)(37), which is reported to be decreased in ASD children (24). Acetate was shown to decrease the permeability of the blood-brain barrier (BBB) in germfree mice (38), which alleviated the severity of social deficits in a mouse model of ASD (39).…”

Characterization of Thermophilic Microorganisms in the Geothermal Water Flow of El Chichón Volcano Crater Lake

“…Thermophiles have optimum growth temperature of >55 • C and in general, many of them are polyextremophiles, which means that they are capable of living in other extreme environmental conditions, such as those related to pH, redox potential, salt concentration or the presence of a wide spectrum of toxic compounds [8][9][10]. Thermophiles are mainly found in hot springs [11][12][13], hot springs adjacent to volcanic environments [14][15][16], upper soil layers [17]-and even in man-made thermal environments, such as compost facilities [18]. Therefore, different research groups have reported important works related with the characterization of microorganisms in aqueous media [19][20][21][22].…”