Tepidicaulis marinus gen. nov., sp. nov., a marine bacterium that reduces nitrate to nitrous oxide under strictly microaerobic conditions

Abstract

Tepidicaulis marinus gen. nov., sp. nov., a marine bacterium that reduces nitrate to nitrous oxide under strictly microaerobic conditions T was most closely related to the genera Rhodobium, Parvibaculum, and Rhodoligotrophos (92-93 % similarity) within the class Alphaproteobacteria. Strain MA2 T was aGram-stain-negative and stalked dimorphic bacteria. The temperature range for growth was 16-48 6C (optimum growth at 42 6C). This strain required yeast extract and NaCl (.1 %, w/v) for growth, tolerated up to 11 % (w/v) NaCl, and was capable of utilizing various carbon sources. The major cellular fatty acid and major respiratory quinone were C 18 : 1 v7c and ubiquinone-10, respectively. The DNA G+C content was 60.7 mol%. Strain MA2 T performed denitrification and produced N 2 O from nitrate under strictly microaerobic conditions. Strain MA2 T possessed periplasmic nitrate reductase (Nap) genes but not membrane-bound nitrate reductase (Nar) genes. On the basis of this morphological, physiological, biochemical and genetic information a novel genus and species, Tepidicaulis marinus gen. nov., sp. nov., are proposed, with MA2 T

Pyruvatibacter mobilis gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122

“…These major cellular fatty acids of strain GYP-11 T are similar to those of members of the genera Parvibaculum (Lai et al, 2011;Rosario-Passapera et al, 2012), Tepidicaulis (Takeuchi et al, 2015) and Parvularcula (Arun et al, 2009;Yu et al, 2013;Li et al, 2014). However, members of the genera Parvibaculum, Tepidicaulis and Parvularcula are characterized by cyclo C 19 : 0 v8c (12.1-35.8 %), C 18 : 1 v7c/v6c (87.3 %) and C 16 : 0 (10.0-24.1 %), respectively.…”

“…Additional substrate, such as Tween 20 or pyruvate, was essential for growth of strain GYP-11 T on MA, while these substrates were not required by Parvibaculum indicum P31 T (Lai et al, 2011) or the recently described Tepidicaulis marinus MA2 T (Takeuchi et al, 2015). Growth of Parvibaculum lavamentivorans DS-1 T on MAP was observed, but was much slower than strain GYP-11 T .…”

“…marinus MA2 T , although no aggregation was reported (Takeuchi et al, 2015). Additional substrate, such as Tween 20 or pyruvate, was essential for growth of strain GYP-11 T on MA, while these substrates were not required by Parvibaculum indicum P31 T (Lai et al, 2011) or the recently described Tepidicaulis marinus MA2 T (Takeuchi et al, 2015).…”

“…Stalked cells were also observed in Tepidicaulis Table 1. Differential phenotypic properties between strain GYP-11 T and some other related genera Taxa: 1, strain GYP-11 T ; 2, Parvibaculum (Schleheck et al, 2004;Lai et al, 2011;Rosario-Passapera et al, 2012); 3, Parvularcula (Cho & Giovannoni, 2003;Arun et al, 2009;Yu et al, 2013;Li et al, 2014); 4, Tepidicaulis (Takeuchi et al, 2015). +, Positive; 2, negative; V, variable between species; W, weakly positive; ND, no data available.…”

Cell cycle regulated DNA methyltransferase: fluorescent tracking of a DNA strand-separation mechanism and identification of the responsible protein motif

“…The organisms listed represent this variety and include human pathogens Rhodobacter massiliensis ( 46 ), Pannonibacter pragmitetus ( 47 ), Inquilinus limosus ( 48 ), Haematospirillum jordaniae ( 49 ) and Methylocapsa palsarum ( 50 ), a bacteria that contributes to biogas production Rhodopseudomonas faecalis ( 51 ), a plant-growth promoting bacteria Azospirillum sp. RU38E ( 52 ), two methanotrophs Methylocapsa palsarum ( 53 ) and Methylocella silvestris ( 54 ), and Tepidicaulis Marinus , which reduces nitrate ( 55 ). Also highlighted are a nitrogen fixing alphaproteobacterium Hartmannibacter diazotrophicus ( 56 ) and Martelella endophytica , which exhibits inhibitory activity against fungal plant pathogens ( 57 ).…”