TEQ-value determinations of animal feed; emphasis on the CALUX bioassay validation

Vanderperren, Huig; Wouwe, Nathalie Van; Behets, S; Windal, Isabelle; Overmeire, Ilse Van; Fontaine, Anthony

doi:10.1016/j.talanta.2004.05.027

Cited by 21 publications

(12 citation statements)

References 9 publications

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“…Extraction and sample clean-up have been shown to retain persistent halogenated compounds such as PCDD/Fs, while PCBs can be examined separately. In general, the results obtained by CALUX overestimate the levels of PCDD/Fs as determined by chemical analysis (Vanderperren et al, 2004). Potential synergistic or antagonistic effects of dioxin-like compounds are thus taken into account (Schoeters et al, 2004;Joung et al, 2007).…”

Section: Discussionmentioning

confidence: 95%

“…CALUX measures the toxicity of the food extract in comparison with dioxin toxicity in terms of activity of the arylhydrocarbon receptor (AhR). The results of the CALUX-bioassay have been compared to results obtained by chemical analysis, such as gas chromatography-high resolution mass spectrometry (GC-HRMS) (Van Overmeire et al, 2000;Vanderperren et al, 2004). Although bioassay results may show a higher variability in comparison to chemical analysis, results of both assays are well correlated (Schoeters et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…The CALUX assay uses a recombinant cell line, which responds to PCDD/Fs and dioxin-like molecules with Ah receptor (AhR)-dependent induction of firefly luciferase in a dose related response. The methodology used is described elsewhere (Vanderperren et al, 2004).…”

Section: Contaminant Concentrationsmentioning

confidence: 99%

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Dietary exposure to dioxin-like compounds in three age groups: Results from the Flemish environment and health study

Bilau

Matthys

Baeyens³

et al. 2008

Chemosphere

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Lipophilic contaminants are present in the environment and bioaccumulate in the food chain. Therefore, their intake via animal fat of various sources was assessed for three age groups of the Flemish population, participating in a large biomonitoring program of the Flemish government. In total, 1636 adolescents (14-15 years), 1186 mothers (18-44 years), and 1586 adults (50-65 years) participated in the study and completed a semi quantitative food frequency questionnaire. Individual consumption data were combined, via a so-called simple distribution approach, with recent data on polychlorinated dibenzo-p-dioxins/furans and dioxin-like polychlorinated biphenyls, measured via the chemical-activated luciferase gene expression (CALUX) bio-assay in food items available on the Flemish market.The median (95th percentile) estimated intakes of dioxin-like contaminants were 2.24 (4.61), 2.09 (4.26), and 1.74 (3.53) pg CALUX-TEQ kg À1 bw d À1 for, respectively adolescents, mothers and adults. These data are in the same range as those found in other European studies. The CALUX-TEQ results of respectively 59.8%, 53.7% and 36.2% of the adolescent, mother and adult population exceed the tolerable weekly intake (TWI) of 14 pg WHO-TEQ kg À1 bw w The main contributors of dioxin-like substances are fish and seafood (25-43% of the total intake), added fats (22-25% of the total intake) and dairy products (17-20% of the total intake).

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Dietary exposure to dioxin-like compounds in three age groups: Results from the Flemish environment and health study

Bilau

Matthys

Baeyens³

et al. 2008

Chemosphere

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“…The application of chemical instruments, for example, high-resolution gas chromatography and mass spectrometry (HR GC/MS) is costly and time-consuming, but also the "gold standard" method for the detection and quantification of dioxin-like compounds (Vanderperren et al, 2004). In the environment, chemicals exist and occur as complex mixtures including different congeners and isomers, with chemical analysis only being able to identify and quantify the chemical for which analytical techniques and standards are available for (Garrison et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…In the environment, chemicals exist and occur as complex mixtures including different congeners and isomers, with chemical analysis only being able to identify and quantify the chemical for which analytical techniques and standards are available for (Garrison et al, 1996). Instrumental analyses also do not account for interactions amongst the chemicals in complex mixtures and provide minimal information on their biological effects (Vanderperren et al, 2004). Hence, chemical analyses may underestimate the potential risks posed by these chemicals; some toxicologically important compounds may be overlooked.…”

Section: Introductionmentioning

confidence: 99%

Dietary exposure to polychlorinated dibenzo-p-dioxins and dibenzofurans via fish consumption and dioxin-like activity in fish determined by H4IIE-luc bioassay

Chan

Man

Xing

et al. 2013

Science of The Total Environment

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PCBs and PCDD/FS in Fish and Fish Products and Their Impact on the Human Body Burden in Belgium

Baeyens

Leermakers

Elskens

et al. 2007

Arch Environ Contam Toxicol

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The concentrations of marker PCBs (28, 52, 101, 118, 138, 153, 180) in fish have been assessed with GC-MS: an average concentration of 540 ng-PCB g(-1) fat (5.02 ng-PCB g(-1) wet weight) was observed. The average concentration of PCDD/Fs, assessed with the CALUX bioassay, amounted to 64 pg-CALUX-TEQ g(-1) fat (0.58 pg-CALUX-TEQ g(-1) wet weight) and that of PCDD/Fs + dioxin-like PCBs amounted to 131 pg-CALUX-TEQ g(-1) fat (1.18 pg-CALUX-TEQ g(-1) wet weight). Results of the PCB congeners analyses show that PCB-153 is the most abundant congener in almost all samples, with also main contributions of the 138- and 180-congeners. For some species such as the sand sole and lemon sole, a fairly constant PCB content, independent of the fat percentage, was observed. For a second group of species such as whelks, cod, and whiting, a positive correlation was observed between their PCB concentration (ng g(-1) fat) and their % of fat. The relationship between marker PCBs and PCDD/Fs concentrations, when plotted on a log scale, fits a straight line (correlation coefficient r = 0.83). With our results on fish and literature data for other food products, intake of marker PCBs and PCDD/Fs could be calculated for the adult population in Belgium (19-60 years old). The Total Daily Intake (TDI) of marker PCBs (ng-PCB day(-1)) ranges between 1690 and 2210. The TDI of PCDD/Fs (pg-CALUX day(-1)) ranges between 80.5 and 122, that of PCDD/Fs + dioxin-like PCBs amounts to 151. When PCDD/Fs in fish are assessed with GC-HRMS, the TDI can be lower. The relative importance of fish regarding marker PCB intake amounts to 15-19%, while regarding PCDD/Fs intake it amounts to 34-51%. Using TDI, the body burden evolution of marker PCBs and PCDD/Fs, with age has been calculated.

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TEQ-value determinations of animal feed; emphasis on the CALUX bioassay validation

Cited by 21 publications

References 9 publications

Dietary exposure to dioxin-like compounds in three age groups: Results from the Flemish environment and health study

Dietary exposure to dioxin-like compounds in three age groups: Results from the Flemish environment and health study

Dietary exposure to polychlorinated dibenzo-p-dioxins and dibenzofurans via fish consumption and dioxin-like activity in fish determined by H4IIE-luc bioassay

PCBs and PCDD/FS in Fish and Fish Products and Their Impact on the Human Body Burden in Belgium

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