Terahertz Imaging of Excised Breast Tumor Tissue on Paraffin Sections

Bowman, Tyler; El-Shenawee, Magda; Campbell, Lucas K.

doi:10.1109/tap.2015.2406893

Cited by 116 publications

(72 citation statements)

References 24 publications

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“…The origin of contrast between diseased and healthy tissue could be different water contents as well as the difference in the cell densities. More recently, in 2015, Bowman et al (39) have investigated the contrast issue by imaging dehydrated breast cancer slides. The samples were formalin fixed, paraffin embedded infiltrating ductal carcinoma (IDC) cut into 10, 20 and 30 μm slides.…”

Section: Ex Vivo Imagingmentioning

confidence: 99%

Recent advances in terahertz technology for biomedical applications

Sun¹,

He²,

Li³

et al. 2017

Quant. Imaging Med. Surg.

230

113

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Terahertz instrumentation has improved significantly in recent years such that THz imaging systems have become more affordable and easier to use. THz systems can now be operated by non-THz experts greatly facilitating research into many potential applications. Due to the non-ionising nature of THz light and its high sensitivity to soft tissues, there is an increasing interest in biomedical applications including both in vivo and ex vivo studies. Additionally, research continues into understanding the origin of contrast and how to interpret terahertz biomedical images. This short review highlights some of the recent work in these areas and suggests some future research directions.

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Section: Ex Vivo Imagingmentioning

confidence: 99%

Recent advances in terahertz technology for biomedical applications

Sun¹,

He²,

Li³

et al. 2017

Quant. Imaging Med. Surg.

230

113

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“…Previous work with FFPE tissue in our group dealt primarily with tissue on glass slides [16,30]. However, this posed a significant problem with regards to the ability to perform transmission spectroscopy of the tissue due to the strong attenuation of glass in the THz range [30].…”

Section: Selection Of Slide Materialsmentioning

confidence: 99%

“…In Fig. 11, the Fourier transform is used to obtain images at specific frequencies that can show additional details as reported in breast infiltrating ductal carcinoma [16]. In this case the areas of both transmission and reflection modes are truncated to the same size across all images for the purpose of comparison.…”

Section: Transmission and Reflection Images Of Lcmentioning

confidence: 99%

“…As a result, THz imaging has shown promise in assessment of burns [9], liver cirrhosis [10], and tissue damage from myocardial infarction [11]. Most notably, THz imaging has been applied to detecting and assessing regions of cancer in healthy tissue for a wide range of cancers including cancer of the skin [12,13], liver [11], colon [14], brain [15], and breast [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

“…Previous work by our group has focused on the reflection imaging of infiltrating ductal carcinoma in flat sections [16], and preliminary results in reflection imaging of threedimensional tissue embedded in paraffin blocks was reported [28,29]. Preliminary work has investigated the THz transmission spectroscopy, but using only few point measurements taken from the same sample [30,31].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Terahertz transmission vs reflection imaging and model-based characterization for excised breast carcinomas

Bowman

El-Shenawee

Campbell

2016

Biomed. Opt. Express

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This work presents experimental and analytical comparison of terahertz transmission and reflection imaging modes for assessing breast carcinoma in excised paraffinembedded human breast tissue. Modeling for both transmission and reflection imaging is developed. The refractive index and absorption coefficient of the tissue samples are obtained. The reflection measurements taken at the system's fixed oblique angle of 30° are shown to be a hybridization of TE and TM modes. The models are validated with transmission spectroscopy at fixed points on fresh bovine muscle and fat tissues. Images based on the calculated absorption coefficient and index of refraction of bovine tissue are successfully compared with the terahertz magnitude and phase measured in the reflection mode. The validated techniques are extended to 20 and 30 μm slices of fixed human lobular carcinoma and infiltrating ductal carcinoma mounted on polystyrene microscope slides in order to investigate the terahertz differentiation of the carcinoma with non-cancerous tissue. Both transmission and reflection imaging show clear differentiation in carcinoma versus healthy tissue. However, when using the reflection mode, in the calculation of the thin tissue properties, the absorption is shown to be sensitive to small phase variations that arise due to deviations in slide and tissue thickness and non-ideal tissue adhesion. On the other hand, the results show that the transmission mode is much less sensitive to these phase variations. The results also demonstrate that reflection imaging provides higher resolution and more clear margins between cancerous and fibroglandular regions, cancerous and fatty regions, and fibroglandular and fatty tissue regions. In addition, more features consistent with high power pathology images are exhibited in the reflection mode images.

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Optimal frequency determination for terahertz technology‐based detection of colitis‐related cancer in mice

Chen,

Li,

Liu

et al. 2023

Journal of Biophotonics

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Colorectal cancer is a prevalent malignancy globally, often linked to chronic colitis. Terahertz technology, with its non‐invasive and fingerprint spectroscopic properties, holds promise in disease diagnosis. This study aimed to explore terahertz technology's application in colitis‐associated cancer using a mouse model. Mouse colorectal tissues were transformed into paraffin‐embedded blocks for histopathological analysis using HE staining. Terahertz transmission spectroscopy was performed on the tissue blocks. By comparing terahertz absorption differences, specific frequency bands were identified as optimal for distinguishing cancerous and normal tissues. The study revealed that terahertz spectroscopy effectively differentiates colitis‐related cancers from normal tissues. Remarkably, 1.8 THz emerged as a potential optimal frequency for diagnosing colorectal cancer in mice. This suggests the potential for rapid histopathological diagnosis of colorectal cancer using terahertz technology.This article is protected by copyright. All rights reserved.

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Terahertz Imaging of Excised Breast Tumor Tissue on Paraffin Sections

Cited by 116 publications

References 24 publications

Recent advances in terahertz technology for biomedical applications

Recent advances in terahertz technology for biomedical applications

Terahertz transmission vs reflection imaging and model-based characterization for excised breast carcinomas

Optimal frequency determination for terahertz technology‐based detection of colitis‐related cancer in mice

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