Teratogenic effects of ethanol exposure on zebrafish visual system development

Arenzana, Francisco Javier; Carvan, Michael J.; Aijón, José; Sánchez-González, Rosario; Arévalo, R.; Porteros, A.

doi:10.1016/j.ntt.2006.02.001

Cited by 124 publications

(123 citation statements)

References 25 publications

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“…[55][56][57][58][59] We have extended these results by using this model to identify a novel molecular mechanism that may be responsible for alcohol's teratogenic effects, namely, alcoholinduced inhibition of the cholesterol modification of Shh, which subsequently inhibits Shh signal transduction; inhibition of this pathway appears to play the key role in the development of FASD pathogenesis. As zebrafish lack placentas and develop ex utero, and alcohol dehydrogenases 60,61 are not expressed in embryos at the time exposed to alcohol (ie from 4-10 hpf), Thus, the metabolites generated by oxidation of ethanol are not likely to be a major cause of the induced phenotypes.…”

Section: Discussionmentioning

confidence: 97%

Fetal alcohol exposure impairs hedgehog cholesterol modification and signaling

Yang

Zdanowicz

et al. 2007

Laboratory Investigation

135

144

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Section: Discussionmentioning

confidence: 97%

Fetal alcohol exposure impairs hedgehog cholesterol modification and signaling

Yang

Zdanowicz

et al. 2007

Laboratory Investigation

135

144

View full text Add to dashboard Cite

“…For over three decades it has been clear that ethanol exerts deleterious effects on the developing zebrafish embryo (e.g., Laale, 1971). Numerous recent studies have investigated the teratogenic and toxicological properties of this substance in zebrafish (Arenzana et al, 2006;Hallare et al, 2006) and zebrafish have also been proposed as a model of fetal alcohol syndrome (Bilotta et al, 2004). The behavioral effects of ethanol have also been studied in the zebrafish embryo (Lockwood et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Effects of acute and chronic ethanol exposure on the behavior of adult zebrafish (Danio rerio)

Gerlai

Lee

Blaser

2006

Pharmacology Biochemistry and Behavior

206

158

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The zebrafish has been a popular subject of embryology and genetic research for the past three decades. Recently, however, the interest in its neurobiology and behavior has also increased. Nevertheless, compared to other model organisms, e.g., rodents, zebrafish behavior is understudied and very few behavioral paradigms exist for mutation or drug screening purposes. Alcoholism is one of the biggest and costliest diseases whose mechanisms are not well understood. Model organisms such as the zebrafish may be utilized in this line of research. Previously, we investigated the effects of acute ethanol exposure on adult zebrafish using four behavioral paradigms and employing manual quantification methods. Here, we study the effects of chronic ethanol exposure and analyze how it modifies the effects of acute ethanol treatment. We employ a videotracking-based automated quantification method in a predator model paradigm and show that this method is capable of detecting an avoidance reaction that is ameliorated by higher doses of ethanol, a potential anxiolytic effect. Importantly, we also demonstrate that chronic, two week long, exposure to ethanol results in significant adaptation to this substance in adult zebrafish. Overall, our results suggest that zebrafish will be an appropriate subject for high throughput screening applications aimed at the analysis of the mechanisms and pharmacology of acute and chronic ethanol induced changes in the vertebrate brain.

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“…Genetic background plays a significant role in the susceptibility to ethanol-induced cyclopia, with the EK background displaying cyclopia in even 1% ethanol. 46 The Wnt/PCP pathway is critically involved in gastrulation movements and genetically interacts with 1% ethanol to produce cyclopia in vangl2 mutants, 47 which also have disrupted Wnt/PCP signaling. In addition, in the highly ethanol-sensitive TL strain, 0.6% ethanol has been shown to alter the movement of cells during gastrulation.…”

Section: Zebrafish Models Of Fasdmentioning

confidence: 99%

Fishing for Fetal Alcohol Spectrum Disorders: Zebrafish as a Model for Ethanol Teratogenesis

2016

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Fetal Alcohol Spectrum Disorders (FASD) describes a wide array of ethanol-induced developmental defects, including craniofacial dysmorphology and cognitive impairments. It affects *1 in 100 children born in the United States each year. Due to the pleiotropic effects of ethanol, animal models have proven critical in characterizing the mechanisms of ethanol teratogenesis. In this review, we focus on the utility of zebrafish in characterizing ethanolinduced developmental defects. A growing number of laboratories have focused on using zebrafish to examine ethanol-induced defects in craniofacial, cardiac, ocular, and neural development, as well as cognitive and behavioral impairments. Growing evidence supports that genetic predisposition plays a role in these ethanol-induced defects, yet little is understood about these gene-ethanol interactions. With a high degree of genetic amenability, zebrafish is at the forefront of identifying and characterizing the gene-ethanol interactions that underlie FASD.

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Teratogenic effects of ethanol exposure on zebrafish visual system development

Cited by 124 publications

References 25 publications

Fetal alcohol exposure impairs hedgehog cholesterol modification and signaling

Fetal alcohol exposure impairs hedgehog cholesterol modification and signaling

Effects of acute and chronic ethanol exposure on the behavior of adult zebrafish (Danio rerio)

Fishing for Fetal Alcohol Spectrum Disorders: Zebrafish as a Model for Ethanol Teratogenesis

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