Teratogenic effects of N‐ethyl‐N‐nitrosourea (ENU) on larvae of the self‐fertilizing fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae)

Lee, Jae Seong; Chang, Sung Yeoul; Kim, Il Chan; Han, Moonsik; Lee, Young Sik; Lee, Yong Sung

doi:10.1002/tcm.10031

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…Mild to acute teratogenic effects were observed in larvae exposed to N ‐ethyl‐ N ‐nitrosourea (Fig. 3) (Lee et al , 2002). At higher dose (8 mM), mortality was also very high (>70%).…”

Section: The Biology Of K Marmoratusmentioning

confidence: 99%

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Kryptolebias marmoratus(Poey, 1880): a potential model species for molecular carcinogenesis and ecotoxicogenomics

Lee

Raisuddin

Schlenk

2008

Journal of Fish Biology

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Kryptolebias marmoratus (Rivulus marmoratus till 2004, order Cyprinodontoformes; family Rivulidae) is the only known self-fertilizing hermaphrodite vertebrate. Because of this uniqueness, there has been great interest in its biology, physiology, developmental biology and genomics along with other basic and applied areas of environmental genomics and molecular carcinogenesis. In laboratory studies, K. marmoratus has shown susceptibility to a number of xenobiotics including carcinogens. Toxicologists are interested in the mechanisms of action of environmental chemicals of contemporary interest such as endocrine disruptors and may consider this as a suitable fish model for the study of chemically induced carcinogenesis. Single short-term exposure to model chemical carcinogens is sufficient to induce tumourigenesis in K. marmoratus in a relatively short time span. In recent years, considerable data have been generated about gene sequences of K. marmoratus. Laboratory maintenance of K. marmoratus is relatively easy. Gene bank data are readily available, though not as extensive as for zebrafish Danio rerio or Japanese madaka Oryzias latipes. The expressed sequence tag (EST) data, differential display real-time reverse-transcriptase polymerase chain reaction and Gene Sequencer 20Ô sequencing data have recently been helpful in understanding the genomics and proteomics of this species. Recently, sequences of the oncogene, ras, and tumour suppressor gene, p53, have been reported in K. marmoratus. The expression of these important genes that play significant roles not only in carcinogenesis but also in other signal transduction pathways was observed to be modulated by environmental endocrine-disrupting chemicals. Having a large sequence database (cDNA sequences and ESTs), it is expected that the use of a microarray in the near future will open new vistas for this fish with applications in cancer research, aquatic toxicology, endocrinology and genomics. This review discusses potential uses of K. marmoratus in new biotechnological approaches of aquatic toxicology.

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Section: The Biology Of K Marmoratusmentioning

confidence: 99%

“…(a) Control, (b) 8 week old fish after exposure to 8 mM N ‐ethyl‐ N ‐nitrosourea. Bar indicates 10 mm long (Figure reproduced with permission from Lee et al , 2002).…”

Section: The Biology Of K Marmoratusmentioning

confidence: 99%

Kryptolebias marmoratus(Poey, 1880): a potential model species for molecular carcinogenesis and ecotoxicogenomics

Lee

Raisuddin

Schlenk

2008

Journal of Fish Biology

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“…This species can be reared individually in small containers with less than 60 ml of artificial seawater by feeding them Artemia nauplii without nutritional enrichment. Taking advantage of these characteristics, mangrove killifish have been used as a clonal model fish for ecotoxicology studies (Lee et al 2002), and behavioral studies related to early stage development (Grageda et al 2005). This fish can be highly useful when investigating the effects of various types of foods since the individuals of one lineage are homozygous clones and thus any deviation in observed traits or characters can be attributed to nutritional or environmental effects and not to the individuals' genotype.…”

Section: Introductionmentioning

confidence: 99%

Feeding behaviour, feed selectivity and growth studies of mangrove killifish, Kryptolebias marmoratus, larvae using various live and formulated feeds

2007

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Nine types of live foods viz. L, S and SS morphotypes of Brachionus plicatilis sp. complex, first instar Artemia franciscana, Fabrea salina, Acartia tsuensis, Tigriopus japonicus, Diaphanosoma celebensis, Moina mongolica and a formulated feed of two sizes (400 and 700 µm) were used to observe feeding behaviour and growth of mangrove killifish, Kryptolebias marmoratus. Behavioural observations were made for one hour on days 0, 1, 5, and 10 after hatching. Focus, unsuccessful and successful attacks and vomit were noted. With rotifers L, S and SS types and newly hatched Artemia nauplii as food, all the larvae showed maximum feeding success throughout the experimental period. Larvae did not consume any of the 700 µm artificial diet. Vomiting was noticed on capturing the ciliate Fabrea and 400 µm artificial diets. Rotifers were ingested in greater numbers. SS-type rotifers were consumed in largest number (209.2/h per individual) on day 10. Significantly greater growth was observed after 10 days rearing with L type rotifer, Artemia nauplii, T. japonicus, A. tsuensis, M. mongolica, D. celebensis, and a mixture of L type rotifer and F. salina (Tukey-Kramer post hoc test, P<0.05). Feed selectivity experiments on days 0, 1, 5 and 10 revealed that killifish larvae feed preferentially on Artemia nauplii and rotifers from a mixture of Artemia nauplii, rotifers, A. tsuensis, T. japonicus, D. celebensis and M. mongolica. Techniques for culturing various zooplankton at small-scales are also described.

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Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphrodite fish Rivulus marmoratus and the Japanese medaka Oryzias latipes

Kim

Yoon

et al. 2004

Marine Environmental Research

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Teratogenic effects of N‐ethyl‐N‐nitrosourea (ENU) on larvae of the self‐fertilizing fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae)

Abstract: We showed that N-Ethyl-N-Nitrosourea (ENU) induces teratogenesis in larvae of the self-fertilizing fish Rivulus marmoratus. We discuss this and the issue of carcinogenesis caused by ENU.

Cited by 6 publications

References 21 publications

Kryptolebias marmoratus(Poey, 1880): a potential model species for molecular carcinogenesis and ecotoxicogenomics

Kryptolebias marmoratus(Poey, 1880): a potential model species for molecular carcinogenesis and ecotoxicogenomics

Feeding behaviour, feed selectivity and growth studies of mangrove killifish, Kryptolebias marmoratus, larvae using various live and formulated feeds

Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphrodite fish Rivulus marmoratus and the Japanese medaka Oryzias latipes

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