Teratogenic study of phenobarbital and levamisole on mouse fetus liver tissue using biospectroscopy

Ashtarinezhad, Azadeh; Panahyab, Ataollah; Shaterzadeh-Oskouei, Shahrzad; Khoshniat, Hessam; Mohamadzadehasl, Baharak; Shirazi, Farshad H.

doi:10.1016/j.jpba.2016.05.015

Cited by 10 publications

(11 citation statements)

References 35 publications

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“…However, it has some downshifts in comparison with the pure PhNT′s FTIR absorption peaks in this area which indicates the interaction between metronidazole and nanotubes [55] . In addition, the fluctuation peaks at 600–899 cm −1 are attributed to the ring deformation vibrations due to the interactions between metronidazole and nanotubes [56,57] …”

Section: Resultsmentioning

confidence: 92%

Synthesis and Characterization of Phenylalanine Nanotubes as Green pH‐Responsive Drug Nanocarriers

et al. 2020

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The present study is aiming to investigate the potential of phenylalanine amino acid nanotubes (PhNTs) synthesized by a self‐assembly process as an efficient and pH‐responsive green drug nanocarrier for the treatment of oral disease. Phenylalanine was used for the preparation of solutions with three different concentrations (0.1, 0.5 and 2.5 mg/ml). Scanning electron microscopy (SEM) and Field emission scanning electron microscopy (FESEM) images showed that low and high concentrations of phenylalanine solution creates nanospheres and nanowires, respectively rather than tubular‐shaped nanostructures. In contrast, phenylalanine nanotubes were synthesized by medium concentration (0.5 mg/ml). The tubular shape and nanoscale diameter of the synthesized PhNTs were characterized by FESEM, Transmission Electron microscopy (TEM), Dynamic Light Scattering (DLS) and Fourier Transform Infrared Spectroscopy (FTIR). These characterization tests confirmed the synthesis of PhNTs with a tubular shape and nanoscale diameter. In the second stage, metronidazole was loaded into the phenylalanine‐based nanostructures as an effective drug for oral disorders. The results indicated that nanospheres and nanowires show drug loading efficiency of 49 % and 58 %, respectively, after 48 h of incubation time. Whereas, the drug loading efficiency for nanotubes was 91 %. The results from the FTIR analysis and UV‐vis spectroscopy verified the presence of metronidazole into the hollow structure of PhNT. While a burst drug release was observed for nanospheres and nanowires, PhNTs showed a well‐controlled release of metronidazole up to 95 % at oral pH, which is best fitted to the Weibull model. Subsequently, the cell viability of phenylalanine‐based nanostructures was assessed by MTT test on L929 fibroblast cell line.

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Section: Resultsmentioning

confidence: 92%

Synthesis and Characterization of Phenylalanine Nanotubes as Green pH‐Responsive Drug Nanocarriers

et al. 2020

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“…In newly diagnosed CNs patients with a similar phenotype, looking for mutations in the HNF1a binding site seems a good strategy. Upon confirming presence of a mutated HNF-1a binding site the use of phenobarbital should be reconsidered in view of its sedative effect and specifically in women, because of its reported teratogenic properties [5].…”

Section: Discussionmentioning

confidence: 99%

A novel UGT1A1 gene mutation causing severe unconjugated hyperbilirubinemia: a case report

et al. 2019

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Background Crigler-Najjar syndrome (CNs) presents as unconjugated hyperbilirubinemia, as a result of UGT1A1 deficiency, and can be categorized in a severe (type I) and mild (type II) phenotype. CNs type II patients usually benefit from phenobarbital treatment that induces residual UGT1A1 activity. Case presentation Here we present a CNs type II patient that is not responsive to phenobarbital treatment, which can be explained by two heterozygous mutations in the UGT1A1 gene. A 3 nucleotide insertion in the HNF-1α binding site in the proximal promoter previously reported in a Crigler-Najjar patient on one allele and a novel two nucleotide deletion in exon 1, resulting in a frameshift and a premature stop codon. Conclusion In newly diagnosed CNs patients with unconjugated bilirubin levels consistent with CNs type II but that are unresponsive to phenobarbital treatment, disruption of the HNF-1α binding site in the proximal promoter should be considered as a probable cause. Upon confirming a mutation in the HNF-1α site, phenobarbital treatment should be stopped or at least be reconsidered because of its sedative effects and its teratogenic properties. Electronic supplementary material The online version of this article (10.1186/s12887-019-1555-y) contains supplementary material, which is available to authorized users.

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“…The fetuses fixed in Bouin׳s fixative solution for 18 h at room temperature, dehydrated (extraction of cells and tissues water) in graded ethanol series, and embedded in paraffin. Sections of fetuses were cut at thickness 10 µM by microtome [3] . Slices were placed on conventional glass slides for staining for Haematoxylin and Eosin (H&E) histological study of fetus abnormalities using optical microscopy.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…Slices were placed on conventional glass slides for staining for Haematoxylin and Eosin (H&E) histological study of fetus abnormalities using optical microscopy. H&E is the common and standard staining method used in medical diagnosis [3] , [4] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

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The effects of radiofrequency radiation on mice fetus weight, length and tissues

et al. 2018

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The public concern of harmful effects of radiofrequency radiation exposure, especially with rapid increase in the use of wireless and telecommunication devices, is increasing. Some studies show fetal and developmental abnormalities as the result of radiofrequency radiation exposure. We aimed to investigate possible teratogenic effects of radiofrequency in 915 MHz on mice fetus and protective role of vitamin C. 21 pregnant mice were divided into 3 groups. Control group was in normal condition without any stressor agent. Exposure group was exposed to 915 MHz RFR (8 h/day for 10 days) and 0.045 µw/cm2 power density. The exposure plus vitamin C group received 200 mg/kg vitamin C by gavage and was exposed to 915 MHz RFR (8 h/day for 10 days) and 0.045 µw/cm2 power density. The fetus weight, C-R length were measured by digital balance and caliper. Tissues were assessed after staining with H & E. Our results showed significant increase in fetus weight and C-R length and also enlarged liver, tail deformation in mice fetus in exposure group. Although usage of vitamin C caused significant decrease in mentioned parameters. The outcome of this study confirms the effects of radiofrequency radiation on growth parameters such as body weight, length and some tissues in mice fetuses and protective effect of vitamin C. However more studies on non-ionization radiation in different frequencies and severity, during pregnancy are needed to clarify the exact mechanisms of these changes and better protection.

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Teratogenic study of phenobarbital and levamisole on mouse fetus liver tissue using biospectroscopy

Cited by 10 publications

References 35 publications

Synthesis and Characterization of Phenylalanine Nanotubes as Green pH‐Responsive Drug Nanocarriers

Synthesis and Characterization of Phenylalanine Nanotubes as Green pH‐Responsive Drug Nanocarriers

A novel UGT1A1 gene mutation causing severe unconjugated hyperbilirubinemia: a case report

The effects of radiofrequency radiation on mice fetus weight, length and tissues

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