Terbium identifies double-stranded RNA on gels by quenching the fluorescence of intercalated ethidium bromide

Al-Hakeem, Mazin; Sommer, Steve S.

doi:10.1016/0003-2697(87)90245-4

Cited by 20 publications

(9 citation statements)

References 18 publications

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“…(1) and that the Ksv values were estimated from these linear plots. The collision fluorescence quenching effect of KI in both the absence and presence of DNA is presented in Fig.…”

Section: Competitive Binding Between Eb and Co-salen For Dnamentioning

confidence: 99%

“…[1][2][3][4][5][6] Some metal complexes, such as Cu-phenanthroline, Fe-(EDTA), Mn-porphyrin, Rupolypyridyl and metal-bis(salicylidene)ethylenediamine (salen), can cleave or bind DNA in the presence of co-oxidants, such as peroxides. [7][8][9][10][11][12] Bhattacharya et al reported a new water-soluble Co-salen complex that can cleave DNA spontaneously under ambient aerobic conditions.…”

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confidence: 99%

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Interaction of Metal Complexes of Bis(salicylidene)-ethylenediamine with DNA

et al. 2000

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Recent years have seen a growing interest in the binding of small molecules to deoxyribonucleic acid (DNA) and DNAcleaving with metal complexes. [1][2][3][4][5][6] Some metal complexes, such as Cu-phenanthroline, Fe-(EDTA), Mn-porphyrin, Rupolypyridyl and metal-bis(salicylidene)ethylenediamine (salen), can cleave or bind DNA in the presence of co-oxidants, such as peroxides. [7][8][9][10][11][12] Bhattacharya et al. reported a new water-soluble Co-salen complex that can cleave DNA spontaneously under ambient aerobic conditions. The cleavage is further enhanced by including dithiothreitol in the reaction buffer. 13 Gravert and co-workers investigated specific DNA cleavage mediated by Mn-salen, and found that the combination of Mn-salen and a terminal oxidant affords efficient and specific cleavage of righthanded double-helical DNA in regions rich in A:T base pairs.14 Routier et al. synthesized a functionalized Cu-salen complex and investigated the DNA-binding and DNA-cleaving properties of this Cu complex using viscometry, UV spectroscopy, circular dichroism and gel electrophoresis. It has been shown the complex induces single-stranded breaks via an interaction within one of the grooves of the double helix. 15 This paper reports on the fluorescence spectra of metal complexes of salen and their interactive effect on DNA. The interactive pattern between metal complexes of salen and DNA was investigated by UV spectroscopy, KI quenching and competitive binding of ethidium bromide (EB) and Co-salen with DNA. Co-salen as a novel fluorescence probe has a potential analytical application for the determination of traces of DNA. ExperimentalCo-salen was synthesized and purified as previously reported. [16][17][18][19] Calf thymus DNA was purchased from Huamei Biotechnological Co. (Beijing) and used as received with the purity checked by the absorbance ratio, A260/A280, of DNA, which should not be less than 1.8 in a 0.2 mol L -1 phosphate buffer solution containing 60 mmol L -1 NaCl. The DNA concentration, expressed in base pairs, was estimated by spectrophotometry using a molar absorptivity value, ε260, of 1.31 × 10 4 mol L -1 cm -1 . Other chemicals were of analytical reagent grade. Doubly distilled water was used throughout. ApparatusAll fluorescence measurements were carried out with an M850 fluorescence spectrophotometer (Hitachi, Japan). Excitation and emission wavelengths of 230 nm and 342 nm were used, respectively. The absorption spectra were recorded with an UV-1100 spectrophotometer (Rayleigh Analytical Instruments, Beijing). ProcedureAll experiments, except where specifically indicated, were carried out at pH 7.0 in a Britton-Robinson buffer containing NaCl (60 mmol L -1) and an appropriate amount of metal complexes of salen. Test solutions of metal complexes were incubated at 250˚C for 4 min after the addition of DNA. The pH values of buffers covering 1.82 -12.17 were measured using a PHS-3C Model ion meter (Shanghai). Results and Discussion Fluorescence spectral characteristics of metal complexes of salenThe e...

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“…(1) and that the Ksv values were estimated from these linear plots. The collision fluorescence quenching effect of KI in both the absence and presence of DNA is presented in Fig.…”

Section: Competitive Binding Between Eb and Co-salen For Dnamentioning

confidence: 99%

mentioning

confidence: 99%

Interaction of Metal Complexes of Bis(salicylidene)-ethylenediamine with DNA

et al. 2000

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“…Recent years have seen growing interest in the employment of small molecules for DNA studies (2)(3)(4)(5). Some small molecules, such as acridines, can be used as biological probes; they are also potential parent compounds for anticancer drugs acting on DNA as the target.…”

Section: Introductionmentioning

confidence: 99%

Studies on Intramolecular Charge Transfer Fluorescence Probe and DNA Binding Characteristics

Yang

Shen

1999

Microchemical Journal

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“…Plots of I0/I vs. iodide concentration for Mn-Schiff-base with various DNA samples indicate that the data follow Eq. (1) and that the Ksv values were estimated from these linear plots. Figure 6 shows the collision fluorescence quenching effect of KI in both the presence and absence of DNA.…”

Section: Ki Quenchingmentioning

confidence: 99%

“…[1][2][3][4][5][6] DNA might interact with small molecules according to three possible patterns: (1) The electrostatic interaction, which extends the external wall of DNA double helix and lacks selectivity; (2) Interaction with grooves of DNA; and (3) intercalation model. Some metal complexes such as Cuphenanthroline, Fe-EDTA, Mn-porphyrin and Ru-polypyridyl, can cleave or bind DNA in the presence of co-oxidants such as peroxides.…”

mentioning

confidence: 99%

Fluorescence Spectral Study of Interaction of Water-soluble Metal Complexes of Schiff-base and DNA

et al. 2001

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Recent years have seen growing interest in the employment of small molecules for deoxyribonucleic acid (DNA) studies. [1][2][3][4][5][6] DNA might interact with small molecules according to three possible patterns: (1) The electrostatic interaction, which extends the external wall of DNA double helix and lacks selectivity; (2) Interaction with grooves of DNA; and (3) intercalation model. Some metal complexes such as Cuphenanthroline, Fe-EDTA, Mn-porphyrin and Ru-polypyridyl, can cleave or bind DNA in the presence of co-oxidants such as peroxides. 7-11 Schiff-bases are potential anticancer drugs, so they are currently studied extensively. [12][13][14][15][16] The anticancer activity of these complexes tends to increase when they complex with metal ions. 17,18 21 Gravert and his co-workers investigated DNA binding/cleaving properties of twenty-six salen complexes of Mn(III) in the presence of terminal oxidants using DNA affinity cleaving techniques, and found DNA cleaving efficiency varying with both the structure and stereochemistry of the bridge. 23 We reported metal complexes of salen interaction of DNA and found that Co-salen showed the strongest interaction of DNA and that the interactive model between Co-salen is an intercalative one. 24 This paper reports on the fluorescence spectra characteristics of water-soluble metal complexes of Schiff-base and their interactive effects on calf thymus DNA under physiological condition. The interactive pattern between metal complexes of Schiff-base and DNA was investigated by UV spectroscopy, KI quenching and competitive binding of ethidium bromide (EB) and Mn-Schiff-base with DNA.The effects of salt concentration and pH of medium on interaction of DNA and metal complexes of Schiff-base have also been investigated. Mn-Schiff-bases as a novel fluorescence probe have potential analytical applications for the determination of traces of DNA. Experimental ReagentsWater-soluble Metal-Schiff-base complexes ( The fluorescence spectral characteristics and the interaction of several water-soluble metal complexes of Schiff-base with DNA are described. Among the complexes tested, Mn-Schiff-base bound to DNA showed a marked decrease in the fluorescence intensity with a blue shift of the excitation and emission peaks. Some hypochromism in the UV absorption spectra was also observed. KI quenching and competitive binding to DNA between Mn-Schiff-base and ethidium bromide (EB) were studied in connection with other experimental observations to show that the interactive model between Mn-Schiff-base and DNA is an intercalative one. The pH and salt effect on the fluorescence properties was also investigated. The linear relationship between F/F0 and the concentration of calf thymus DNA covers 3.0 × 10 -6 -2 × 10 -4 mol L -1 , which can be utilized for determining traces of calf thymus DNA with a detection limit of 8.0 × 10 -7 mol L -1 in base pairs.

show abstract

Terbium identifies double-stranded RNA on gels by quenching the fluorescence of intercalated ethidium bromide

Cited by 20 publications

References 18 publications

Interaction of Metal Complexes of Bis(salicylidene)-ethylenediamine with DNA

Interaction of Metal Complexes of Bis(salicylidene)-ethylenediamine with DNA

Studies on Intramolecular Charge Transfer Fluorescence Probe and DNA Binding Characteristics

Fluorescence Spectral Study of Interaction of Water-soluble Metal Complexes of Schiff-base and DNA

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