Teripang Sebagai Salah Satu Sumber Kolagen

Kumayanjati, Bayu

doi:10.14203/oseana.2020.vol.45no.1.51

Cited by 10 publications

(13 citation statements)

References 3 publications

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“…12 Previous studies stated that the protein content in sea cucumbers can reach 40%, 13 from several species of sea cucumbers analyzed the protein content is quite high, ranging from 31.11% to 42.32%. 14 The high protein content confirms that sea cucumbers and Tilapia-skin have considerable potential as a source of collagen. Collagen is part of the protein that has many benefits.…”

Section: Resultsmentioning

confidence: 75%

Amino Acid and Proximate Analysis of Type-1 Collagen from Sea Cucumber and Tilapia-Skin and its Potential Application as Artificial Tendon

Safira¹,

Rani²,

Puspitasari³

et al. 2022

Pharmacogn. J.

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The dried sea cucumbers were washed with distilled water, dissected, and soaked in a solution of 0.5 N NaCl, 0.2 N -mercaptoethanol, 50 mM EDTA, and 0.1 N Tris-HCl. Next, the mixture was centrifuged at 8000 rpm and added with 0, 1 N NaOH. The sediment was centrifuged again and washed with distilled water. The supernatant obtained, added pepsin (1 g/100 g collagen) with 0.5 N acetic acid, centrifuged, and added NaCl to 0.6 M. By centrifugation, the sediment was redissolved in 0.5

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Section: Resultsmentioning

confidence: 75%

Amino Acid and Proximate Analysis of Type-1 Collagen from Sea Cucumber and Tilapia-Skin and its Potential Application as Artificial Tendon

Safira¹,

Rani²,

Puspitasari³

et al. 2022

Pharmacogn. J.

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“…These properties are present in CMG1418 alginate (Skjåk-Bræk et al, 1989 ; Storz et al, 2009 ). Based on viscosity, alginates have been classified into three categories: low viscosity alginate (< 240 mPas), medium viscosity alginate (240–3500 mPas), and high viscosity alginate (> 3500 mPas) (Rasyid, 2003 ). Thus, according to this classification, CMG1418 alginate could be classified as high viscosity alginate.…”

Section: Resultsmentioning

confidence: 99%

Promising substitute of inconsistent algal alginates: exploring the biocompatible properties of di-O-acetylated, poly-L-guluronate-deficient alginate from soil bacterium Pseudomonas aeruginosa CMG1418

Muhammadi¹,

Shafiq²,

Rizvi³

2023

bta

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The structural inconsistencies in commercial algal alginates have limited their reliability and quality for various applications. Therefore, the biosynthesis of structurally consistent alginates is crucial to replace the algal alginates. Thus, this study aimed to investigate the structural and alginate's structural and functional properties of Pseudomonas aeruginosa CMG1418 as a substitute. To achieve this, the CMG1418 alginates were physiochemically characterized using various techniques such as transmission electron microscopy, Fourier-transform infrared 1 H-NMR, 13 C-NMR, and gel permeation chromatography. The synthesized CMG1418 alginate was then subjected to standard tests to evaluate its biocompatibility, emulsification, hydrophilic, flocculation, gelling, and rheological properties. The analytical studies revealed that CMG1418 alginate is an extracellular and polydisperse polymer with a molecular weight range of 20 000-250 000 Da. It comprises 76% poly-(1-4)-β-D-mannuronic acid (M-blocks), no poly-α-L-guluronate (G-blocks), 12% alternating sequences of β-D-mannuronic acid and α-L-guluronic acid (poly-MG/GM-blocks), 12% MGM-blocks, 172 degrees of polymerization, and di-O-acetylation of M-residues. Interestingly, CMG1418 alginate did not show any cytotoxic or antimetabolic activity. Moreover, compared to algal alginates, CMG1418 alginate exhibited higher and more stable flocculation efficiencies (70-90%) and viscosities (4500-4760 cP) over a wide range of pH and temperatures. Additionally, it displayed soft to flexible gelling abilities and higher water-holding capacities (375%). It also showed thermodynamically more stable emulsifying activities (99-100%) that surpassed the algal alginates and commercial emulsifying agents. However, only divalent and multivalent cations could slightly increase viscosity, gelling, and flocculation. In conclusion, this study explored a structurally di-O-acetylated and poly-G-blocks-deficient, biocompatible alginate, and its pH and thermostable functional properties. This research suggests that CMG1418 alginate is a superior and more reliable substitute for algal alginates in various applications, such as viscosifying, soft gelling, flocculating, emulsifying, and waterholding.

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“…Semua data penelitian dicatat menggunakan scuba slate. Spesies kima yang tidak teridentifikasi langsung, didokumentasikan lalu ditulis cirinya secara detail untuk kemudian akan diidentifikasi menggunakan buku identifikasi terumbu karang (Knop, 1996;Dermawan et al 2015;Kumayanjati, 2015).…”

Section: Prosedur Penelitianunclassified

Density and Composition of Species Clam (Tridacna sp.) Based on Coral Cover Conditions in Wawosunggu Waters, Konawe Regency

Iskandar

Ishak²,

Oetama³

2023

JBT

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Kima is one of the protected marine resources. It has an ecological role as a water bio-filter and a symbiotic mutualism with coral reef ecosystems. The purpose of the research was to find out of species density and composition of clams (Tridacna sp.) based on the coral coverage on the waters island of Wawosunggu, South Konawe. The study was conducted in September 2022. Sampling was carried out at 3 observation stations based on habitat characteristics. The observing method used a transect belt/swept area. Data in the form of density, relative abundance of clams, and percentage of coral cover analyze by using MS software. Excel 2010. The number of clams obtained was 155 individuals consisting of 3 types, namely Tridacna maxima, T. squamosa, and T. crocea. The totally density (totals from species and locations) was 0.10 individuals/m2. The composition of all types of clams ranges from 25-56%. The percentage of coral cover obtained was grouped into 4 categories, namely fleshy seaweed/algae, hard coral, abiotic, and other biotic. The percentage of coral cover at all stations was dominated by the hard coral category (Acropora sp. and non Acropora sp.) with water quality conditions that supported the presence of clams and coral reefs.

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Teripang Sebagai Salah Satu Sumber Kolagen

Cited by 10 publications

References 3 publications

Amino Acid and Proximate Analysis of Type-1 Collagen from Sea Cucumber and Tilapia-Skin and its Potential Application as Artificial Tendon

Amino Acid and Proximate Analysis of Type-1 Collagen from Sea Cucumber and Tilapia-Skin and its Potential Application as Artificial Tendon

Promising substitute of inconsistent algal alginates: exploring the biocompatible properties of di-O-acetylated, poly-L-guluronate-deficient alginate from soil bacterium Pseudomonas aeruginosa CMG1418

Density and Composition of Species Clam (Tridacna sp.) Based on Coral Cover Conditions in Wawosunggu Waters, Konawe Regency

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