Terminal deoxynucleotidyl transferase in a case of PH1 positive infant chronic myelogenous leukaemia

Saffhill, Roy; Dexter, T. M.; Muldal, S.; Testa, Nydia G; Jones, Peter; Joseph, Ansamma

doi:10.1038/bjc.1976.105

Cited by 12 publications

(4 citation statements)

References 18 publications

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“…If this is exact, NALM-I would represent the first cell line belonging to the lymphoid series of cells which contains Phl chromosome. Although Ph' chromosome is regarded as being characteristic of CML, it has also been detected in other situations (Sandberg and Hossfield, 1970) including ALL (Propp and Lizzi, 1970;Peterson et al, 1976;Walker and Hardy, 1976) and terminal transferase activity, although always high in non-T/non-B or T cell leukemic lymphoblasts (Hutton and Coleman, 1976;Srivastava and Minowada, 1975), has also been found in large amounts in rare cases of AML (Srivastava et nl., 1976), myeloblast-like cells from rare cases of blastic phase CML (Srivastava et al, 1977), acute undifferentiated leukemia, rare cases of acute myelomonocytic leukemia ) and a single case of chronic phase CML (Saffhill et al, 1976 …”

Section: Resultsmentioning

confidence: 99%

“…Terminal transferase catalyzes the polymerization of deoxyribonucleotide on the 3'-hydroxyl ends of oligo or polydeoxyribonucleotide initiators in the absence of a template (Bollum, 1974). Although terminal transferase, which occurs in large amounts in thymus, was considered to be specific for that tissue (Bollum, 1974), this enzyme has been recently found in other tissues (McCaffrey et ul., 1975;Sarin et al, 1976;Saffhill et al, 1976;Hutton and Coleman, 1976;Srivastava, 1974Srivastava, , 1975. Determinations of terminal transferase activity and cell surface markers have been recently used for examining the origin of leukemic cells and established cell lines and their characterization (Hutton and Coleman, 1976;Srivastava and Minowada, 1975: Seliaman et al.…”

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confidence: 99%

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Terminal deoxynucleotidyl transferase activity and cell surface antigens of two unique cell lines (nalm‐1 and balm‐2) of human leukemic origin

Srivastava

Minowada

1977

Intl Journal of Cancer

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Two unique cell lines, NALM-1 and BALM-2 derived from lymphoblast-like cells of chronic myelogenous leukemia and rare B cell acute lymphoblastic leukemia patients, respectively, were compared with fresh parent cells from the patients and with a Philadelphia chromosome positive K-562 cell line previously established from a chronic myelogenous leukemia patient in blastic phase. NALM-1 resembled the parent cells in the presence of Philadelphia chromosome, non-T/non-B acute lymphoblastic leukemia specific antigens and lack of T or B cell markers, whereas BALB-2, like the parent cells, had two chromosome markers and bore kappa, delta and mu immunoglobulins. NALM-1 lacked Epstein-Barr virus genome, whereas BALM-2 showed the presence of Epstein-Barr virus genome. K-562 cells lacked all the antigen markers examined. All cells had high DNA polymerase alpha activity and low DNA polymerase gamma activity. NALM-1, like the parent cells and unlike K-562 cells, had high terminal deoxynucleotidyl transferase activity of about 200 mu/mg DNA, whereas BALM-2, like its parent cells, had terminal deoxynucleotidyl transferase activity of 1-2 mu/mg DNA (1 u = 1 nmole Mn++-dGTP/h on dA12-18 initiator). Terminal deoxynucleotidyl transferase was characterized by its chromatographic and sedimentation behavior, thermal sensitivity and specific inhibition by streptolydigin and terminal deoxynucleotidyl transferase antisera. These results indicate that NALM-1 and K-562 may represent different phenotypes of cells in CML blastic crisis. Moreover, NALM-1 and BALM-2 seem to have retained the characteristics of original leukemic cells from which they may have been derived.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Terminal deoxynucleotidyl transferase activity and cell surface antigens of two unique cell lines (nalm‐1 and balm‐2) of human leukemic origin

Srivastava

Minowada

1977

Intl Journal of Cancer

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“…High TdT levels have been found in the normal thy mus [6,19] and a trace of enzyme activity has been detected in cells from normal bone marrow [1,6,10,14]. Increased TdT activ ity has been reported to be present in almost all cases of ALL [3,5,6,10,13,14,16,19,20], as well as in a number of cases of chronic myelocytic leukemia (CML) in biastic crisis [3,5,6,13,14,17,19,23,24], in lym phoblastic lymphoma [7,16] and in rare cases of acute myelocytic leukemia (AML) [3,25],…”

Section: Introductionmentioning

confidence: 99%

Terminal Transferase in Leukemia of Adults

Ga¹,

Beutler²

1979

Acta Haematol

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Terminal deoxynucleotidyl transferase (TdT) determinations were carried out on peripheral blood leukocytes or bone marrow cells from 61 adult patients with various types of leukemia. TdT activity was undetectable in the cells of patients with acute myeloeytic or acute myelomonocytic leukemia but was present in 12 of 13 patients with acute nonmyelocytic leukemia. None of 3 patients with acute myelocytic transformation of chronic myelocytic leukemia (CML) manifested TdT activity while 4 of 6 patients with lymphoid transformation had such activity.More patients with TdT in their leukemic cells responded to treatment than those without TdT activity. However, our findings suggest that TdT activity may be less useful in management of leukemia than has sometimes been supposed.

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“…ALLllymphoblastische NHL (4,5,6,7,9) common-ALL (6), non-B/non-T-ALL (10,18) prä-T-ALL, T-ALL (10,18) prä-B-ALL (16,18) AUL 7Iymphoblastische NHL ohne leukämischen Verlauf CML, Blastenkrise (12,13) ANLL (7,18) kortikale Thymozyten Knochenmark BI utlymphozyten…”

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Darstellung der terminalen Deoxynukleotidyl-Transferase im histologischen Schnitt*

Steinmann¹,

Müller-Hermelink²

1983

Klin Padiatr

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Terminal deoxynucleotidyl transferase (TdT) is a unique DNA-polymerase which is restricted to a few organs (thymus, bone marrow and blood). Therefore, the enzyme is suited for the role of a tumor marker in all other organs. The immunohistological demonstration, hitherto, was mainly hampered by the lack of potent and specific antibodies and by the readiness of the enzyme to diffuse. Now, these problems are solved. The immunohistological demonstration of TdT can be used to classify acute leukemias and lymphoblastic non-Hodgkin-lymphomas of children and to early recognize relapse situations.

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Terminal deoxynucleotidyl transferase in a case of PH1 positive infant chronic myelogenous leukaemia

Cited by 12 publications

References 18 publications

Terminal deoxynucleotidyl transferase activity and cell surface antigens of two unique cell lines (nalm‐1 and balm‐2) of human leukemic origin

Terminal deoxynucleotidyl transferase activity and cell surface antigens of two unique cell lines (nalm‐1 and balm‐2) of human leukemic origin

Terminal Transferase in Leukemia of Adults

Darstellung der terminalen Deoxynukleotidyl-Transferase im histologischen Schnitt*

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