Terminal proteins essential for the replication of linear plasmids and chromosomes in <i>Streptomyces</i>

Bao, Kai; Cohen, Stanley N.

doi:10.1101/gad.896201

Cited by 104 publications

(131 citation statements)

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“…Eventually, the complete nucleotide sequence (356 023 bp) of SCP1 was determined, 17 which confirmed the size of TIRs (75 122 bp) and located the mmy gene cluster, a replication origin, two partition genes (parA and parB) and others. However, a terminal protein gene (tpg) 18 was not identified from the sequence data, because of the unique telomere sequence of SCP1. Later, Huang et al 19 isolated the terminal protein as a complex with SCP1 and finally identified the tpc (terminal protein of SCP1, orf127) and tac (telomere-associated protein of SCP1, orf125) genes of SCP1.…”

Section: Giant Linear Plasmids In Streptomycesmentioning

confidence: 99%

Giant linear plasmids in Streptomyces: a treasure trove of antibiotic biosynthetic clusters

Kinashi

2010

J Antibiot

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Many giant linear plasmids have been isolated from Streptomyces by using pulsed-field gel electrophoresis and some of them were found to carry an antibiotic biosynthetic cluster(s); SCP1 carries biosynthetic genes for methylenomycin, pSLA2-L for lankacidin and lankamycin, and pKSL for lasalocid and echinomycin. Accumulated data suggest that giant linear plasmids have played critical roles in genome evolution and horizontal transfer of secondary metabolism. In this review, I summarize typical examples of giant linear plasmids whose involvement in antibiotic production has been studied in some detail, emphasizing their finding processes and interaction with the host chromosomes. A hypothesis on horizontal transfer of secondary metabolism involving giant linear plasmids is proposed at the end.

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Section: Giant Linear Plasmids In Streptomycesmentioning

confidence: 99%

Giant linear plasmids in Streptomyces: a treasure trove of antibiotic biosynthetic clusters

Kinashi

2010

J Antibiot

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“…This protein falls into the class of TPs that include TpgR1, TpgR2 and Tpgr3 from S. rochei, TpgL from S. lividans, TpgC from S. coelicolor and TpgSLP2 from the 50 kb linear plasmid SCP2 of S. lividans (Bao & Cohen, 2001Yang et al, 2002). The tpgR1 gene mapped to the S. rochei chromosome and the tpgR2 and tpgr3 genes mapped to the 206 kb and 100 kb linear plasmids within S. rochei (Bao & Cohen, 2001). The TP homologue TpgSV2, identified on pSLV45, is most probably also involved in protein-primed DNA synthesis for plasmid replication.…”

Section: Sequence Analysis and Deduced Functions Of Pslv45 Proteinsmentioning

confidence: 99%

“…SLV.25, designated tpgSV45, encodes a putative protein of 185 aa with very strong similarity (52 % identity, 62 % positives), over its entire length, to TpgR2, a recently described 59 terminal binding protein from S. rochei (Bao & Cohen, 2001). This protein falls into the class of TPs that include TpgR1, TpgR2 and Tpgr3 from S. rochei, TpgL from S. lividans, TpgC from S. coelicolor and TpgSLP2 from the 50 kb linear plasmid SCP2 of S. lividans (Bao & Cohen, 2001Yang et al, 2002). The tpgR1 gene mapped to the S. rochei chromosome and the tpgR2 and tpgr3 genes mapped to the 206 kb and 100 kb linear plasmids within S. rochei (Bao & Cohen, 2001).…”

Section: Sequence Analysis and Deduced Functions Of Pslv45 Proteinsmentioning

confidence: 99%

“…Type I restriction systems have both restriction and modification activities in one heteromeric enzyme and function as a defence mechanism protecting bacterial cells from foreign DNA. SLV.6 contains both M and S type I restriction regions (Cooper & Dryden, 1994 Bao & Cohen, 2001Yang et al, 2002). The tpgR1 gene mapped to the S. rochei chromosome and the tpgR2 and tpgr3 genes mapped to the 206 kb and 100 kb linear plasmids within S. rochei (Bao & Cohen, 2001).…”

Section: Sequence Analysis and Deduced Functions Of Pslv45 Proteinsmentioning

confidence: 99%

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Characterization of the Streptomyces lavendulae IMRU 3455 linear plasmid pSLV45

Hosted¹,

Wang²,

Horan³

2004

Microbiology

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Streptomyces lavendulae IMRU 3455 contains two large linear plasmids designated pSLV45 (45 kb) and pSLV195 (195 kb). A cosmid, pSPRX604, containing 42 kb from pSLV45 was cloned and sequenced. pSLV45 was tagged with a hygromycin-resistance marker by homologous recombination to generate the derivatives pSLV45.680 and pSLV45.681. An apramycin-resistance marker was introduced into S. lavendulae IMRU 467 using the pSPR910 integration vector to yield the recipient strain SPW910. The self-transmissible nature of pSLV45 was determined by transfer of pSLV45.680 and pSLV45.681 from the donor strains SPW680 and SPW681 into the recipient strain SPW910. Southern analysis indicated the presence of hygromycin-and pSLV45-hybridizing sequences within SPW910 exconjugants. PFGE analysis confirmed pSLV45.680 and pSLV45.681 were transferred intact and formed freely replicating linear plasmids. Sequence analysis of pSPRX604 revealed genes predicted to be involved in plasmid transfer, partitioning and regulation. The transfer of the linear plasmid pSLV45 from S. lavendulae IMRU 3455 into S. lavendulae IMRU 467 may allow the development of pSLV45 as an actinomycete-to-actinomycete conjugative shuttle vector. INTRODUCTIONLinear plasmids with 59 terminally attached proteins (TPs) and terminal inverted repeats (TIRs) have been found in bacteria, plants, filamentous fungi and yeasts, and often within actinomycetes (Griffiths, 1995;Hinnebusch & Tilly, 1993;Meinhardt et al., 1990;Rohe et al., 1992). Actinomycete linear plasmids pSLA1 (17 kb) and pSLA2 (17 kb) were first detected in the lankacidin-producer Streptomyces sp. 7434-AN4 and in Streptomyces rochei, respectively (Hayakawa et al., 1979;Hirochika & Sakaguchi, 1982;Hirochika et al., 1984). Both plasmids were shown to contain TIRs and TPs (Hirochika & Sakaguchi, 1982;Hirochika et al., 1984). With the advent of PFGE analysis, other actinomycete linear plasmids have been identified including Streptomyces lividans SLP2 (50 kb), Streptomyces clavuligerus pSCL1 (12 kb), Streptomyces avermitilis pSA1 and pSA2 (100 kb and 250 kb), Streptomyces rimosus pZG101 (387 kb), Streptomyces lasaliensis pKSL (520 kb) and Streptomyces coelicolor SCP1 (363 kb) Evans et al., 1994;Gravius et al., 1994;Keen et al., 1988;Kinashi & Shimaji, 1987;Kinashi & Shimaji-Murayama, 1991;Kinashi et al., 1994). All these plasmids were found to contain TIRs and TPs. In addition, both pSLA2 and SLP2 were shown to contain an internal origin of replication for bi-directional replication of DNA (Chang & Cohen, 1994;Chang et al., 1996;Huang et al., 2003).Unlike the chromosomes of most bacteria, which are circular in nature, the chromosomes of actinomycetes are linear (Lezhava et al., 1995;Lin et al., 1993). This was first demonstrated in S. lividans and has now been established for several other actinomycetes including S. coelicolor, Streptomyces griseus and Saccharopolyspora erythraea (formerly Streptomyces erythraeus) (Lezhava et al., 1995;Lin et al., 1993;Reeves et al., 1998). All actinomycete chromosomes characterized to da...

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“…Their 'telomeres' contain inverted repeat sequences from 44 to 180 kb long Pandza et al, 1998). The 39 telomeric ends are free, but the 59 telomeric ends are blocked by covalently linked telomere terminal proteins (Bao & Cohen, 2001;Yang et al, 2002). Many Streptomyces linear plasmids have conserved telomeric sequences (e.g.…”

Section: Introductionmentioning

confidence: 99%

Three functional replication origins of the linear and artificially circularized plasmid SCP1 of Streptomyces coelicolor

et al. 2013

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Previous reports showed that the large linear plasmid SCP1 of Streptomyces coelicolor A3(2) contains a 5.4 kb centrally located replication locus. We report here that SCP1 actually contains three internal replication loci. Subcloning of the 5.4 kb sequence identified a 3.2 kb minimal locus (rep1A/repB/iteron) that determined propagation in Streptomyces lividans. The two newly identified replication genes, rep2A and rep3A, resembled the rep gene of Streptomyces circular plasmid pZL12. Transcription start points of the three replication genes were determined. The three replication loci could independently determine propagation in linear mode in S. lividans. Individual and sequential deletions of the rep1A and rep3A genes were successful. The SCP1-derived linear plasmids with deletions of the rep1A and/or rep3A genes still propagated in similar copy numbers, were inherited largely stable and were transferred efficiently by conjugation in S. coelicolor. Interestingly, SCP1 can be artificially circularized to yield a 280 kb circular plasmid, circular SCP-1 (C-SCP1), which contains the three replication loci. Strikingly, the copy numbers, inheritance and transfer of C-SCP1 resembled that of the linear SCP1 plasmids. Transcripts of the rep1A, rep2A and rep3A genes in linear or artificially circularized SCP1 were detected at all the time points of strain growth.

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Terminal proteins essential for the replication of linear plasmids and chromosomes in Streptomyces

Cited by 104 publications

References 65 publications

Giant linear plasmids in Streptomyces: a treasure trove of antibiotic biosynthetic clusters

Giant linear plasmids in Streptomyces: a treasure trove of antibiotic biosynthetic clusters

Characterization of the Streptomyces lavendulae IMRU 3455 linear plasmid pSLV45

Three functional replication origins of the linear and artificially circularized plasmid SCP1 of Streptomyces coelicolor

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