Terminalia ferdinandiana Exell: Extracts inhibit Shewanella spp. growth and prevent fish spoilage

Wright, Mitchell Henry; Shalom, Joseph; Matthews, Ben; Greene, Anthony Carlson; Cock, Ian Edwin

doi:10.1016/j.fm.2018.10.006

Cited by 49 publications

(38 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, all of the T. ferdinandiana extracts screened in this study had high antioxidant capacities and this is believed to be due to their high ascorbic acid contents [30,31]. High ascorbic acid concentrations have profound effects on Artemia nauplii viability [23,32]. Therefore, an evaluation of toxicity for high antioxidant samples using the Artemia nauplii assay alone may provide an unrealistic quantification of extract toxicity.…”

Section: Resultsmentioning

confidence: 94%

“…As the T. ferdinandiana fruit and leaf extracts all displayed good growth inhibitory activity against both S. aureus strains, all were selected for metabolomic phytochemical fingerprint analysis. Several recent studies have reported potent growth inhibitory activity of T. ferdinandiana extracts against multiple bacterial species and have correlated the activity to high tannin and flavonoid contents [16,22,23]. Therefore, we utilized LC-MS metabolomics fingerprinting to detect tannin and flavonoid components with known antibacterial activity that have previously been detected in similar extracts.…”

Section: Resultsmentioning

confidence: 99%

“…against gastrointestinal [15] and skin pathogens [16], as well as against the bacterial triggers of some autoimmune diseases including rheumatoid arthritis, ankylosing spondylitis and multiple sclerosis [17,22]. T. ferdinandiana extracts are also effective inhibitors of food spoilage bacteria [23]. However, none of those studies have yet screened the T. ferdinandiana extracts against ESBL bacteria or against MRSA.…”

Section: Terminalia Ferdinandiana Fruit and Leaf Extracts Inhibit Metmentioning

confidence: 99%

See 2 more Smart Citations

Terminalia ferdinandiana Fruit and Leaf Extracts Inhibit Methicillin-Resistant Staphylococcus aureus Growth

et al. 2019

Self Cite

View full text Add to dashboard Cite

The development of multiple antibiotic–resistant bacteria has vastly depleted our repertoire of effective antibiotic chemotherapies. The development of multi-β-lactam-resistant strains are particularly concerning due to our previous reliance on this class of antibiotics because of their initial efficacy and broad-spectrum activity. With increases in extended-spectrum β-lactam-resistance and an expanded resistance to other classes of antibiotics, there is an urgent need for the development of effective new antibiotic therapies. Terminalia ferdinandiana is an endemic Australian plant known for its high antioxidant and tannin contents. T. ferdinandiana fruit and leaf extracts have strong antibacterial activity against a wide variety of bacterial pathogens. However, T. ferdinandiana extracts have not been tested against ESBL and MRSA antibiotic-resistant pathogens. An objective of this study was to screen T. ferdinandiana fruit and leaf extracts for bacterial growth inhibitory activity by disc diffusion assay against β-lactam-sensitive and -resistant E. coli strains and against methicillin-sensitive and -resistant S. aureus. The minimum inhibitory concentration (MIC) was quantified by liquid dilution techniques. The fruit methanolic extract, as well as the methanolic, aqueous, and ethyl acetate leaf extracts strongly inhibited the growth of the MRSA, with MICs as low as 223 µg/mL. In contrast, the extracts were ineffective inhibitors of ESBL growth. Metabolomic fingerprint analysis identified a diversity and relative abundance of tannins, flavonoids, and terpenoids, several of which have been reported to inhibit MRSA growth in isolation. All extracts were nontoxic in the Artemia nauplii and HDF toxicity assays, further indicating their potential for medicinal use.

show abstract

Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Terminalia Ferdinandiana Fruit and Leaf Extracts Inhibit Metmentioning

confidence: 99%

See 1 more Smart Citation

Terminalia ferdinandiana Fruit and Leaf Extracts Inhibit Methicillin-Resistant Staphylococcus aureus Growth

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Antimicrobial activity of all plant extracts was determined using a modified disc diffusion assay. 27,28 Briefly, 100µL of each bacterial culture was grown in 10mL of fresh nutrient broth until they reached a count of ~10 8 cells/mL. A volume of 100µL of the bacterial suspension was spread onto nutrient agar plates and extracts were tested for antibacterial activity using 5mm sterilised filter paper discs.…”

Section: Evaluation Of Antimicrobial Activitymentioning

confidence: 99%

Brachychiton acerifolius (A.Cunn. ex G.Don) Macarthur and C. moore Leaf and Flower Extracts Inhibit the Growth of a Panel of Pathogenic Bacteria

Cock¹

2019

Self Cite

View full text Add to dashboard Cite

Introduction: Brachychiton acerifolius (A.Cunn. ex G.Don) Macarthur and C. moore is a large tree that is native to subtropical regions of the east of Australia. Decoctions and infusions produced from the leaves of the related species Brachychiton diversifolius were used by the first Australians to treat a variety of bacterial diseases. Despite this, B. acerifolius leaf and flower extracts have not been rigorously examined for antibacterial properties against many pathogens. Methods: The antimicrobial activity of B. acerifolius leaf and flower extracts was investigated by disc diffusion and growth time course assays against a panel of pathogenic bacteria. The growth inhibitory activity was quantified by MIC determination. Toxicity was determined using the Artemia franciscana nauplii bioassay. Results: Methanolic B. acerifolius leaf and flower extracts inhibited the growth of a wide range of bacterial species. Growth of both gram positive and gramnegative bacteria was inhibited by the B. acerifolius leaf and flower extracts, although the gram negative bacteria were substantially more susceptible to the extracts than the gram positive bacteria were. The leaf extracts were more potent growth inhibitors than the flower extracts against all bacteria tested. The B. acerifolius leaf extract was a strong inhibitor of A. faecalis, K. pneumonia, P. fluorescens, S. newport, S. sonnei, B. cereus, S. aureus and S. pyogenes growth, with MIC values substantially <1000µg/mL. The antibacterial activity of the methanolic B. acerifolius leaf extract was further investigated by growth time course assays which showed significant growth inhibition in cultures of P. fluorsecens and B. cereus within 1hr of exposure. Both extracts were determined to be nontoxic in the Artemia franciscana nauplii bioassay, indicating their safety for internal use as well as for topical uses. Conclusion: The lack of toxicity of the B. acerifolius leaf and flower extracts and their growth inhibitory bioactivity against a panel of pathogenic bacteria partially validate the traditional usage of this species to treat bacterial diseases and indicates their potential in the development of antiseptic agents.

show abstract

“…16 Despite this, relatively few studies have rigorously examined the antibacterial activity of Australian native plants, although there has recently been a substantial increase in interest in this field. [21][22][23][24] The genus Westringa (family Lamiaceae) consists of more than 30 species of Australian shrubs. 25 Westringa fruticosa (Willd.)…”

Section: Introductionmentioning

confidence: 99%

Westringa fruticosa (Willd.) Druce Leaf and Flower Extracts Lack Antibacterial Activity and are Non-toxic in vitro

Chikowe¹,

Mpala²,

Cock³

2019

Self Cite

View full text Add to dashboard Cite

Introduction: Closely related plant species often share similar secondary metabolites and bioactivities and are therefore good targets for bioactivity testing when one or more species within a family are known to possess therapeutic properties. The family Laminaceae has a long history of medicinal usage globally. Many species are known to have therapeutic properties, several species of which have well established antibacterial bioactivities. Methods: The ability of Westringa fruticosa leaf and flower extracts to inhibit the growth of a panel of bacterial and fungal pathogens was investigated by disc diffusion assay. Toxicity was examined using the Artemia franciscana nauplii bioassay. Results: W. fruticosa leaf methanolic and aqueous extracts were both completely ineffective at inhibiting the growth of gram-positive and gram-negative panels of bacteria, as well as fungi. The extracts were non-toxic or of low toxicity in the Artemia bioassay following 24 h exposure. Conclusion: Despite the taxonomic relationship to several bioactive Laminaceae spp., W. fruticosa leaf and flower extracts were completely ineffective bacterial and fungal growth inhibitors. However, these extracts may have other therapeutic properties and testing against protozoa, virus and tumour cells is required.

show abstract

Terminalia ferdinandiana Exell: Extracts inhibit Shewanella spp. growth and prevent fish spoilage

Cited by 49 publications

References 31 publications

Terminalia ferdinandiana Fruit and Leaf Extracts Inhibit Methicillin-Resistant Staphylococcus aureus Growth

Terminalia ferdinandiana Fruit and Leaf Extracts Inhibit Methicillin-Resistant Staphylococcus aureus Growth

Brachychiton acerifolius (A.Cunn. ex G.Don) Macarthur and C. moore Leaf and Flower Extracts Inhibit the Growth of a Panel of Pathogenic Bacteria

Westringa fruticosa (Willd.) Druce Leaf and Flower Extracts Lack Antibacterial Activity and are Non-toxic in vitro

Contact Info

Product

Resources

About