Termination sites T1 and T2 from the Escherichia coli chromosome inhibit DNA replication in ColE1-derived plasmids

Pelletier, Anthony J.; Hill, Thomas M.; Kuempel, Peter L.

doi:10.1128/jb.171.3.1739-1741.1989

Cited by 18 publications

(9 citation statements)

References 20 publications

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“…1A, left; for reviews, see references 5, 28, and 97 and, most recently, reference 214). Discovery of a trans-acting factor required for replication arrest at Ter sites in vitro (99,131,233,270) was soon followed by cloning of the tus gene, which encodes the 35.8-kDa protein Tus (102). Recombinant Tus was necessary and sufficient to arrest replication in vitro, and the leading strand arrest site was mapped right at the edge of the Tus binding site (100).…”

Section: Dna Binding Proteinsmentioning

confidence: 99%

Replication Fork Stalling at Natural Impediments

Mirkin

2007

Microbiol Mol Biol Rev

458

466

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SUMMARY Accurate and complete replication of the genome in every cell division is a prerequisite of genomic stability. Thus, both prokaryotic and eukaryotic replication forks are extremely precise and robust molecular machines that have evolved to be up to the task. However, it has recently become clear that the replication fork is more of a hurdler than a runner: it must overcome various obstacles present on its way. Such obstacles can be called natural impediments to DNA replication, as opposed to external and genetic factors. Natural impediments to DNA replication are particular DNA binding proteins, unusual secondary structures in DNA, and transcription complexes that occasionally (in eukaryotes) or constantly (in prokaryotes) operate on replicating templates. This review describes the mechanisms and consequences of replication stalling at various natural impediments, with an emphasis on the role of replication stalling in genomic instability.

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Section: Dna Binding Proteinsmentioning

confidence: 99%

Replication Fork Stalling at Natural Impediments

Mirkin

2007

Microbiol Mol Biol Rev

458

466

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“…Once small DNA fragments containing TerA and TerB as well as the two TerR sites were available, it was shown that they could block replication forks in ColE1 plasmids in vivo (139,FIG. 3.…”

Section: Components Of the Replication Termination System The Terminamentioning

confidence: 99%

Replication Termination inEscherichia coli: Structure and Antihelicase Activity of the Tus-TerComplex

Neylon

Kralicek

Hill

et al. 2005

Microbiol Mol Biol Rev

134

144

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The arrest of DNA replication in Escherichia coli is triggered by the encounter of a replisome with a Tus protein-Ter DNA complex. A replication fork can pass through a Tus-Ter complex when traveling in one direction but not the other, and the chromosomal Ter sites are oriented so replication forks can enter, but not exit, the terminus region. The Tus-Ter complex acts by blocking the action of the replicative DnaB helicase, but details of the mechanism are uncertain. One proposed mechanism involves a specific interaction between Tus-Ter and the helicase that prevents further DNA unwinding, while another is that the Tus-Ter complex itself is sufficient to block the helicase in a polar manner, without the need for specific protein-protein interactions. This review integrates three decades of experimental information on the action of the Tus-Ter complex with information available from the Tus-TerA crystal structure. We conclude that while it is possible to explain polar fork arrest by a mechanism involving only the Tus-Ter interaction, there are also strong indications of a role for specific Tus-DnaB interactions. The evidence suggests, therefore, that the termination system is more subtle and complex than may have been assumed. We describe some further experiments and insights that may assist in unraveling the details of this fascinating process

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“…This gene encodes a DNA-binding protein (Tus protein) ofMr 35,800 that forms a complex with the chromosomal ter sequences, causing inhibition of DNA replication fork progression (7). Arrest of DNA replication in the plasmid R6K is also dependent on the product of the tus gene (8)(9)(10).…”

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confidence: 99%

Escherichia coli Tus protein acts to arrest the progression of DNA replication forks in vitro.

Hill

Marians²

1990

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

117

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A polar DNA replication barrier is formed when the DNA-binding protein Tus forms a complex with any of the four 23-base-pair terminator (ter) sites found in the terminus region of the Escherichia coli chromosome. We have used a plasmid DNA replication system reconstituted with purified proteins in vitro to investigate the interaction ofthe Tus protein with the replication fork. Purified Tus protein alone is necessary and sufficient to arrest DNA replication on CoIEltype plasmid templates containing ter sites. Tus proteincatalyzed termination depends upon the orientation of the ter site in the plasmid DNA. Nucleotide resolution mapping of the terminated nascent DNA shows that leading-strand DNA synthesis arrests at the point of contact with the Tus protein, while the rial lagging-strand primer sites are 50-70 nucleotides upstream. In addition, the distribution of leading-strand arrest sites changes when the composition of the proteins on the lagging-strand side of the replication fork is altered.

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Termination sites T1 and T2 from the Escherichia coli chromosome inhibit DNA replication in ColE1-derived plasmids

Cited by 18 publications

References 20 publications

Replication Fork Stalling at Natural Impediments

Replication Fork Stalling at Natural Impediments

Replication Termination inEscherichia coli: Structure and Antihelicase Activity of the Tus-TerComplex

Escherichia coli Tus protein acts to arrest the progression of DNA replication forks in vitro.

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