Termiticidal activity of chitinase enzyme of Bacillus licheniformis, a symbiont isolated from the gut of Globitermes sulphureus worker

Hussin, Nurul Akmar; Majid, Amran Ab.

doi:10.1016/j.bcab.2020.101548

Cited by 15 publications

(9 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The precipitation was gathered using crossing two distinct phases of until the colloidal chitin reached a pH of 7, the filter cloth was continuously rinsed with normal water. The produced colloid of chitin was squeezed between the filter paper (to reduce any residual moisture) and subsequently kept at 4 °C until it was used again [ 97 ].…”

Section: Methodsmentioning

confidence: 99%

Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential

Mahmoud,

Kilany,

Hassan

2023

BMC Microbiol

View full text Add to dashboard Cite

Background Toxigenic fungi (Aspergillus and Fusarium) and their metabolites represent the major cause of corn and corn-based products contamination and consequently lead to severe economic and health issues. Aim Our current study aimed to investigate the efficacy of using L. macroides Bac6 as a biological control agent against the toxigenic fungi; A. flavus f10 and F. proliferatum f30 and their mycotoxins. Results The results illustrated that A. flavus f10 produced the aflatoxins AFB1 and AFG2 with concentrations of 21.239 and 13.593 ppb, respectively. While F. proliferatum f30 produced fumonisin B1 (9600 ppb). Furthermore, L. macroides showed a high potential for inhibition of toxigenic fungal growth using a dual culture method. F. proliferatum f30 and A. flavus f10 were found to be inhibited by a percentage of 80 and 62.5%, respectively. The results were confirmed using the scanning electron microscope. The antagonistic bacteria, L. macroides, showed chitinase productivity and activity of 26.45 U/L and 0.12 U/mL/min, respectively, which illustrates its potential application as a biocontrol agent. The GC-MS analysis revealed an abundance of Pyrrolo[1,2-a] pyrazine-1,4-dione, Hexahydro in the bacterial supernatant that exhibited antifungal characteristics. L. macroides had a significant reduction of AFB1 and AFG2 produced by A. flavus f10, recording 99.25% and 99% inhibition, respectively. It also showed strong inhibition of fumonisin B1 (90% inhibition) produced by F. proliferatum f30. Conclusion: Thus, the current study is a prospective study evaluating for the first time the potential impact of L. macroides Bac6 against the toxigenic fungi and their toxins.

show abstract

Section: Methodsmentioning

confidence: 99%

Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential

Mahmoud,

Kilany,

Hassan

2023

BMC Microbiol

View full text Add to dashboard Cite

show abstract

“…Most studies focus on the prevalence of subterranean termites in urban and suburban environments. There is limited data on mature subterranean termite reservoirs in the non-agricultural ecosystems surrounding urban areas [ [1] , [2] , [3] , [4] ].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic relationship of subterranean termite Coptotermes gestroi (Blattodea: Rhinotermitidae) inhabiting urban and natural habitats

Vellupillai,

Ab Majid

2024

Heliyon

Self Cite

View full text Add to dashboard Cite

“…Specifically, entomopathogens of Bacillus spp. have been reported to exert termiticidal effect through among other factors, their proteolytic and chitinolytic activities [28,30]. The prospect of proteolytic and chitinolytic bacterial entomopathogens as biocontrol alternative to chemical insecticides is based on enzymatic degradation of the cuticle layer which is a vital structural component for the survival of insect pests [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Entomopathogenic Potential of <em>Bacillus velezensis</em> CE 100 for the Biological Control of Termite Damage in Wooden Architectural Buildings of Korean Cultural Heritage

Moon¹,

Ajuna²,

Won³

et al. 2023

Preprint

View full text Add to dashboard Cite

Biocontrol strategies are gaining tremendous attention with the growing awareness of the irreparable harm caused by the continuous use of synthetic pesticides. This study examines the proteolytic and chitinolytic activities of Bacillus velezensis CE 100 and its termiticidal effect through cuticle degradation. The proteolytic and chitinolytic activities of B. velezensis CE 100 systematically increased with cell growth to the respective peak of 68.3, and 128.3 units/mL after seventh days of inoculation, corresponding with the highest cell growth of 16 x 107 colony forming units (CFU)/mL. The in vitro termiticidal assay showed that B. velezensis CE 100 caused a rapid and high rate of termite mortality with the median lethal time (LT50) of &gt; 1h, and the highest mortality rate of 91.1% and 92.2% recorded at 11h and 12h in the bacterial broth culture and crude enzyme fraction, respectively. In addition to broken setae and deformed sockets, termites treated with the bacterial broth culture exhibited degraded epicuticles, while the crude enzyme fraction caused severe disintegration of both the epicuticle and endocuticle. These results indicate a tremendously higher prospect of B. velezensis CE 100 in the biological control of subterranean termites compared to the previously used entomopathogenic bacteria.

show abstract

Termiticidal activity of chitinase enzyme of Bacillus licheniformis, a symbiont isolated from the gut of Globitermes sulphureus worker

Cited by 15 publications

References 27 publications

Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential

Antifungal activity of Lysinibacillus macroides against toxigenic Aspergillus flavus and Fusarium proliferatum and analysis of its mycotoxin minimization potential

Phylogenetic relationship of subterranean termite Coptotermes gestroi (Blattodea: Rhinotermitidae) inhabiting urban and natural habitats

Entomopathogenic Potential of <em>Bacillus velezensis</em> CE 100 for the Biological Control of Termite Damage in Wooden Architectural Buildings of Korean Cultural Heritage

Contact Info

Product

Resources

About