Ternary Cu(II) complexes: synthesis, structural elucidation, and biological studies (DNA binding, cleavage, antibacterial, cytotoxicity)

Sharada, L. N.; Sunitha, S. N. T.; Sabha, Maryam; Viveka, T. Lakshmi; Yerramilli, Aparna; Prasad, M.

doi:10.1007/s11164-020-04140-9

Cited by 9 publications

(3 citation statements)

References 64 publications

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“…[ 24 ] The exchange interaction coupling constant (G) was derived from the formula, ‘G = g ‖ −2.0023/ g ⊥ −20 023’ and the value was found to be 6.05, which confirms negligible exchange interaction. [ 25 ]…”

Section: Resultsmentioning

confidence: 99%

“…Cell lines are treated with 10% of foetal bovine serum, 1% of penicillin-streptomycin and 1% of l-glutamine for normal healthy cell growth, avoiding contamination. Cell number was counted by haemocytometer and were then seeded into 96 well plates with a density of 5 Â 10 3 cells per well in 100-μl medium and incubated overnight in an environment of 5% CO 2 at 37 C. Varied concentrations (5,10,25,50, 75 and 100 μg/ml) of test compounds and cisplatin (control) (except normal cells) were treated with cell lines over night. A 30 μl of MTT dye was added to each well and incubated for three more hours.…”

Section: In Vitro Cytotoxicity and Mtt Studiesmentioning

confidence: 99%

“…[24] The exchange interaction coupling constant (G) was derived from the formula, 'G = g k À2.0023/g ⊥ À20 023' and the value was found to be 6.05, which confirms negligible exchange interaction. [25] EPR isotropic parameters g iso = 2.124 and A iso = 118 were derived from the spectrum recorded at room temperature. The well-resolved four lines in the ESR spectrum indicate the presence of mononuclear complex with mononuclear copper ion as proved by the magnetic moment value obtained for the complex (1.08BM).…”

Section: Spectral Characterizationmentioning

confidence: 99%

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DNA interaction, molecular dynamics simulation, molecular docking, biological, in vivo anti‐inflammatory and thermal studies of o‐hydroxyacetophenone and 2‐fluoroaniline Schiff base complexes

Maddikayala

Bengi

Reddy

2022

Applied Organom Chemis

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Series of transition metal complexes [Fe (HL) 2 Cl(H 2 O)](1), [Co (HL) 2 ](2), [Ni (HL) 2 ](3) and [Cu (HL) 2 ](4) were derived on condensation of Schiff base ligand o-hydroxyacetophenone with 2-fluoroaniline in 1:1 ratio (HL) and characterized by spectral and analytical techniques. Based on the studies, complex 1 was found to be octahedral and complexes (2), ( 3) and ( 4) square planar. Coats-Redfern calculations suggest that all the complexes are nonspontaneous and thermally stable. DNA binding studies revealed intercalation binding mode, while intrinsic binding constant (K b ) and Stern-Volmer quenching constant (K sq ) support high binding abilities. The nuclease activity using CT-DNA was studied by gel electrophoresis. Docking studies were deduced using Accelry's Discovery Studio 2.1 program to understand binding affinities.The MD simulation, carried in order to analyse the stability of ligand and human topoisomerase I (PDB ID: 1T8I) complex for 100 ns using the Desmond 2020.1 from Schrödinger, LLC, revealed stable conformation of the ligand. In vivo, carrageenan induced inflammation studies in paw volume were carried out in rats, and results showed a higher rate of oedema inhibition for complex 4. Cytotoxicity and antimicrobial assay reveal that complex 4 exhibited higher cytotoxic and antimicrobial activity over other complexes and the ligand.

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Section: Resultsmentioning

confidence: 99%

Section: In Vitro Cytotoxicity and Mtt Studiesmentioning

confidence: 99%