Terpenoid, Benzenoid, and Phenylpropanoid Compounds in the Floral Scent of Vanda Mimi Palmer

Mohd-Hairul, Ab. Rahim; Namasivayam, Parameswari; Lian, Gwendoline Ee Cheng; Abdullah, Janna Ong

doi:10.1007/s12374-010-9123-x

Cited by 39 publications

(36 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cytochrome p450 proteins have been reported to be involved in the modification of monoterpene compound into another compound, for example conversion of linalool to linalool oxide (Hsiao et al 2006;Dudareva et al 2004;Dudareva and Pichersky 2000). Thus, VMPCyP450 was postulated to be involved in the fragrance biosynthesis pathway of Vanda Mimi Palmer since linalool oxide was detected as one of the volatiles of (Kim et al 2008) Vanda Mimi Palmer (Mohd-Hairul et al 2010). Besides, cytochrome p450 has been reported to be involved in other fragrance biosynthesis pathways including benzenoid/phenylpropanoid and lipoxygenase pathways (Ehlting et al 2006).…”

Section: Relative Expression Analysis Of Vmpcmek and Vmpcyp450 Transcmentioning

confidence: 99%

“…In Vanda Mimi Palmer, volatile analyses using GC-MS showed that the fragrance emission profile of this orchid was at the highest level in the early afternoon and dominated by terpenoid (ocimene and linalool), benzenoid (benzyl acetate and methylbenzoate), and phenylpropanoid (phenylethanol and phenylethyl acetate) compounds (Mohd-Hairul et al 2010). So far, only one fragrancerelated cDNAs has been isolated and characterized from Vanda Mimi Palmer, which is 1-deoxy-D-xylulose 5-phosphate reductoisomerase (VMPDXR) (Chan et al 2009).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer

et al. 2011

Self Cite

View full text Add to dashboard Cite

The aims of this study were to isolate and characterize putative fragrance-related cDNAs from the floral cDNA library of Vanda Mimi Palmer, an orchid hybrid that has won several international awards for its sweet fragrance. A total of 1,000,000 pfu were screened by hybridizing cDNA library plaques with fully open flower cDNA probe of Vanda Mimi Palmer representing all mRNAs expressed during daytime. The clones that gave positive signals were in vivo excised and PCR-amplified inserts were subjected to reverse-Northern analysis by hybridizing with cDNA probes of fully open flower of Vanda Mimi Palmer (fragrant orchid) and its bud or fully open flower of Vanda Tan Chay Yan (non-fragrant orchid) separately. The clones up-regulated in fully open flower stage of Vanda Mimi Palmer compared to its bud stage or fully open flower of Vanda Tan Chay Yan were sequenced. Sequence analyses showed the presence of eight putative fragrance-related cDNAs of which two were putative Vanda Mimi Palmer 4-(cytidine 5 0 -diphospho)-2-Cmethyl-D-erythritol kinase (VMPCMEK) and Vanda MimiPalmer cytochrome P450 protein (VMPCyP450). These two transcripts were selected for full-length cDNA isolation and expression analysis by real-time RT-PCR. The VMPCMEK transcript encodes a polypeptide of 400 amino acid residues, while the VMPCyP450 encodes 538 amino acid residues. Relative expression analysis of VMPCMEK and VMPCyP450 transcripts by real-time RT-PCR showed up-regulated expressions in floral tissues compared to vegetative tissues, and both were found to be developmentally regulated.

show abstract

Section: Relative Expression Analysis Of Vmpcmek and Vmpcyp450 Transcmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although it is known that the color of a flower is the first and foremost cue for pollinator s attraction, odors may play a major role for their visits, both as long range attraction cues and at short distances as orientation cues among different flower parts or among different flowers 1,2 . The correct landing site and orientation of pollinators are very important requirements for an effective pollination, as demonstrated by the so-called honey-guides and other colored floral structures 3,4 .…”

Section: Introductionmentioning

confidence: 99%

Flowers Volatile Profile of a Rare Red Apple Tree from Marche Region (Italy)

et al. 2014

View full text Add to dashboard Cite

In this paper, the volatiles emitted by flowers and various parts of the flower of a rare spontaneous Italian red (peel and flesh) apple named "Pelingo", were analyzed by SPME with the aim of identifying the contribution of each one to the whole aroma profile. Linalool was the most abundant volatile of flowers: from 43.0% in the flower buds, to 17.6% in the stylus and stigma headspace. The second most represented volatile was (E,E)-α-farnesene mainly emitted by the mature flowers (32.2%). Benzenoid compounds also have been identified: benzyl-alcohol is the most representative (1.0-16.5%) in all the samples except flower buds, while benzyl acetate (5.7%) and methyl salicylate (7.7%) are mainly present in the calyx and in the mature flowers respectively but not in the flower buds. Benzenoid compounds are the attractors for pollinator, probably for this reason were not detected in the headspace of flower bud

show abstract

“…Don., is a famous ornamental potted plant cultivated in Malaysia selectively for its fragrance characteristic [3,4]. Mohd-Hairul et al [5] found that the floral scent of V. Mimi Palmer was predominated by terpenoid, benzenoid, and phenylpropanoid compounds using GC-MS analyses (Table 1).…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning, Sequencing, and Characterization of a Putative Acetyl-CoA-C-acetyltransferase cDNA from a Highly Fragrant Orchid Hybrid Vanda Mimi Palmer

2012

Self Cite

View full text Add to dashboard Cite

tessellata (Roxb.) Hk.f. ex G. Don, is cultivated as a potted ornamental plant mainly for its fragrance rather than its look. Plant acetyl-CoA-C-acetyltransferase (ACA) is involved in the condensation of two acetyl-CoAs to form acetoacetyl-CoA, which condenses with another acetyl-CoA to yield a crucial molecule, 3-hydroxyl-3-methylglutaryl-CoA, at the initial step of the mevalonate (MVA) pathway. An ACA gene from vandaceous orchid has never been reported. We describe the isolation and molecular characterization of an ACA-like gene from V. Mimi Palmer (designated as VMPACA) to facilitate a better understanding of the terpenoid biosynthesis pathway in orchids. The deduced VMPACA encodes a 376-amino-acid protein with a molecular weight of 39 kDa, which comprises an open reading frame of 1128 bp. It is flanked by 87 bp of 5 -untranslated region and 174 bp of 3 -untranslated region including a poly-A tail. Its protein sequence is 81% identical to other plant ACAs and contains a thiolase active site. The fluctuation expression pattern of VMPACA transcript by real-time RT-PCR showed that it is developmentally and temporally regulated with predominant expression in outer and lateral inner tepals compared to vegetative tissues.

show abstract

Terpenoid, Benzenoid, and Phenylpropanoid Compounds in the Floral Scent of Vanda Mimi Palmer

Cited by 39 publications

References 20 publications

Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer

Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer

Flowers Volatile Profile of a Rare Red Apple Tree from Marche Region (Italy)

Molecular Cloning, Sequencing, and Characterization of a Putative Acetyl-CoA-C-acetyltransferase cDNA from a Highly Fragrant Orchid Hybrid Vanda Mimi Palmer

Contact Info

Product

Resources

About