Terpenoides com atividade antifúngica para Candida Berkhout, causadoras de infecções hospitalares

Abstract

ResumoO aumento da incidência de fungos causadores de infecções hospitalares está associado, em geral, ao emprego e utilização da antibioticoterapia de largo-espectro, na prematuridade, no tempo de exposição e permanência nas unidades de internação. Os terpenoides constituem um vasto grupo de substâncias químicas biossintetizadas a partir da via do mevalonato, extraídas de produtos naturais. Possuem diversas atividades biológicas bem definidas, dentre as quais a antifúngica. O presente trabalho teve como objetivo avaliar a atividade antifúngica de terpenoides frente a espécies de Candida causadoras de infecções hospitalares. Foram realizados testes de atividade antifúngica para a determinação da concentração inibitória mínima pela técnica de microdiluição em placa de 15 terpenoides frente a 11 espécies do gênero Candida. Das 15 substâncias -terpenoides -testadas pela técnica da microdiluição em placa com a determinação da Concentração Inibitória Mínima, 9 (60%) apresentaram atividade antifúngica frente a espécies de Candida e 6 (40%) não apresentaram atividade. Dessas 9 substâncias, 2 (22,2%) apresentaram boa atividade antifúngica e 7 (77,8%) apresentaram atividade antifúngica de moderada a fraca. Os resultados mais promissores foram obtidos com os terpenoides (+) Limoneno com CIM de 46,87 µg/mL para C. krusei e C. guilliermondii e com o terpenoide (-) Limoneno com CIM de 46,87 µg/mL para C. krusei. Palavras-chave: Candida. Terpenos. Infecção Hospitalar. AbstractThe increased incidence of fungi causing nosocomial infections is associated, in general, to employment and use of broadspectrum antibiotic therapy in preterm in exposure time and stay in inpatient units. Terpenoids are a large group of chemicals biosynthesized from mevalonate pathway, extracted from natural products. They have several biological activities well defined among which antifungal ones. This study aimed to evaluate the antifungal activity of terpenoids against Candida species causing nosocomial infections. Tests were performed to determine antifungal minimum inhibitory concentration by microdilution plate 15 terpenoids against 11 species of the genus Candida. Of the 15 substances -terpenoids -tested by the broth microdilution with the determination of Minimum Inhibitory Concentration, 9 (60%) showed antifungal activity against Candida species and 6 (40%) showed no activity. These two substances 9 (22.2%) showed good antifungal activity and 7 (77,8%) showed antifungal activity of moderate to weak. The most promising results were obtained with the terpenoids (+) limonene with MIC of 46.87 µg/mL for C. krusei and C. guilliermondii and the terpenoid (-) limonene with MIC of 46.87 µg/mL for C. krusei.

Chemical Composition and Antifungal Activity of Essential Oils of Rosmarinus officinalis L. and Salvia officinalis L. against Botrytis cinerea Pers

“…All substances rich in terpenoids are considered natural antifungals. 76,77 The compounds of EOs can act alone or in synergy with other compounds with a promise of greater effectiveness. 30,78,79 With respect to the effects of the different types of treatments with REO against tomato leaf rot, the combined preventive and curative treatment was the most effective, resulting in 100% inhibition at concentration of 10 µL/mL (Figure 1A), followed by the curative treatment which gave 100% inhibition at concentration of 20 µL/mL, then the preventive treatment while the simultaneous treatment was the least effective producing slightly above 50% inhibition of the rot at 10 µL/mL (Figure 1A).…”

Antimicrobial and antioxidant activity of the leaves and flowers of Tabebuia aurea and Cordia glabrata

“…No O extrato hexânico das folhas de C. glabrata revelou uma fraca atividade para Candida albicans. Pesquisas realizadas com Cordia verbenacea revelam alguns grupos de compostos fenólicos, além de taninos condensados e terpenos, encontrados em extratos e frações hexânicas de folhas desta espécie vegetal Santos & Matias, 2014, Viriato, 2014. Estes metabólitos os quais apresentam potencial antifúngico, poderiam justificar, se presentes, a fraca, porém observada, atividade anti-C. albicans verificada para o extrato hexânico de C. glabrata.…”

Antifungal potential of essential oils from two varieties of Citrus sinensis (lima orange and bahia navel orange) in postharvest control of Rhizopus stolonifer (Ehrenb.: Fr.) Vuill.

“…It may also be implied that the high antifungal activity exhibited by EOs from lima orange results from the high concentration of limonene, which was 95.2% (Table 1). This monoterpene has already shown promising activity against several types of fungi, such as different species of Candida (Viriato, 2014). On the other hand, Chee et al (2009) described limonene as a potent antifungal against Trichophyton rubrum.…”