Terpinen-4-ol treatment maintains quality of strawberry fruit during storage by regulating sucrose-induced anthocyanin accumulation

Li, Zhenbiao; Wei, Yingying; Xu, Yangyang; Han, Peipei; Jiang, Shu; Xu, Feng; Wang, Hongfei; Tao, Nengguo; Shao, Xingfeng

doi:10.1016/j.postharvbio.2020.111461

Cited by 20 publications

(7 citation statements)

References 41 publications

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“…2,3-Butanedione acts on the cell membrane of pathogenic fungi to produce an inhibitory effect. This is not only consistent with our results, but also consistent with other reports on the effects of antifungal substances ( Cui et al, 2021 ; Li et al, 2021 ).…”

Section: Resultssupporting

confidence: 94%

Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione

Ling

Luo²,

Zhao³

et al. 2023

Front. Microbiol.

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Fungal pathogen contamination is one of the most important factors affecting the postharvest quality and shelf life of wolfberry fruits. Therefore, the prevention and control of fungal pathogens that cause fruit rot has become particularly important. Volatile antifungal agents of biological origin have broad application prospects. They may be safer and more efficient than traditional physical and chemical methods. Four pathogenic fungi were isolated and purified from rotting wolfberry. These pathogenic fungi were determined to be Mucor circinelloides LB1, Fusarium arcuatisporum LB5, Alternaria iridiaustralis LB7, and Colletotrichum fioriniae LB8. In vitro fumigation experiments showed that 2,3-butanedione can effectively inhibit the mycelial growth, spore germination, and sporulation ability of pathogenic fungi. The scanning electron microscope (SEM) showed morphological changes in hyphae. Propidium iodide (PI) Staining and leakage of 260 and 280 nm-absorbing increased, suggesting damage to cell membranes. Furthermore, 2,3-butanedione was found to significantly improve fruit firmness, soluble solid, total phenol, flavonoid, and soluble sugar content, as well as higher SOD enzyme activity and lower PPO and POD enzyme activity in the treated fruit, indicating that 2,3-butanedione can effectively reduce the adverse effects of pathogenic fungi in wolfberry. Based on these results, we conclude that 2,3-butanedione is effective against infection by pathogenic fungi in post-harvest wolfberry. 2,3-butanedione should be considered a viable substitute for conventional fungicides that are currently used to control rot in wolfberry.

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Section: Resultssupporting

confidence: 94%

Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione

Ling

Luo²,

Zhao³

et al. 2023

Front. Microbiol.

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“…Cyanidin-3-glucoside was accumulated in Clematis pitcheri shoots in response to high sucrose concentrations [18]. Pelargonidin 3-glucoside, pelargonidin 3-rutinoside, pelargonidin 3-malonylglucoside, and pelargonidin 3-methylmalonyglucoside increased in postharvest strawberry fruit treated with 50 mM sucrose [21]. Sucrose, glucose, fructose, and sorbitol induced similar degrees of cyanidin-3-O-rutinoside and cyanidin-3-O-glucoside accumulation in the fruit of the red-blushed apricot cultivar [30].…”

Section: Discussionmentioning

confidence: 99%

“…Glucose more effectively induced anthocyanin accumulation than sucrose and without exogenous sugar treatment in blackberry fruit [20]. Strawberry fruit treated with sucrose displayed high levels of pelargonidin derivatives and upregulation of the genes participating in the phenylpropanoid and flavonoid pathways [21]. In Arabidopsis, sucrose induces anthocyanin accumulation in a concentration-dependent manner [22].…”

Section: Introductionmentioning

confidence: 99%

Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro

Wang

Cao

Wang

et al. 2022

Plants

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Anthocyanins are important pigments in peach fruit and are beneficial to human health. Sugars are both energy-storing and signaling molecules and their roles in inducing anthocyanin biosynthesis have received a great deal of research attention. However, the mechanism by which sugars induce anthocyanin biosynthesis in peach fruit is unknown. In order to understand this induction mechanism, comprehensive transcriptome and metabolome were performed in fruit flesh treated with four different sugars for 12 and 24 h, respectively. Here, we found that cyanidin-3-O-(6-O-p-coumaroyl) glucosides accumulated in fruit flesh treated with glucose, sucrose, sorbitol, and fructose in vitro. Two key structural genes of the anthocyanin biosynthesis pathway, namely, PpDFR and PpUFGT, were upregulated in the flesh of sugar-treated peach fruit. By contrast, the two main transcription factors (TFs) PpMYB10.1 and PpBL regulating anthocyanin biosynthetic genes in peach fruit were not upregulated accordingly. Interestingly, two MYB family genes (PpMYB6 and PpMYB44-like) and three bHLH family genes (PpbHLH35, PpbHLH51, and PpbHLH36-like) were upregulated. A dual luciferase assay revealed that PpMYB6 strongly activated the PpUFGT promoter when it was co-infiltrated with PpbHLH35, PpbHLH51, and PpbHLH36-like. When PpMYB44-like was co-infiltrated with PpbHLH35, it also potently activated the PpUFGT promoter. The results of this study help clarify the molecular mechanisms by which glucose, sucrose, sorbitol, and fructose regulate anthocyanin accumulation in peach fruit.

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“…Treated strawberries were incubated for 96 h at 20 °C and 90% relative humidity. The decay index was determined at 48, 72, and 96 h, as described by Li et al Briefly, at each time point, each fruit was ranked from 0 to 4 on the following disease scale: 0, no visible surface infection; 1, ≤25%; 2, 25–50%; 3, 50–75%; and 4, ≥75%. The decay index was calculated using the following formula: …”

Section: Methodsmentioning

confidence: 99%

The Jasmonic Acid Signaling Pathway is Associated with Terpinen-4-ol-Induced Disease Resistance against Botrytis cinerea in Strawberry Fruit

Wei

Cao

et al. 2021

J. Agric. Food Chem.

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Terpinen-4-ol, the main component of tea tree oil, markedly increases the disease resistance of postharvest strawberry fruit. To understand the mechanism underlying the enhancement of disease resistance, a high-throughput RNA-seq was used to analyze gene transcription in terpinen-4-ol-treated and untreated fruit. The results show that terpinen-4-ol induces the expression of genes in the jasmonic acid (JA) biosynthesis pathway, secondary metabolic pathways such as phenylpropanoid biosynthesis, and pathways involved in plant-pathogen interactions. Terpinen-4-ol treatment reduced disease incidence and lesion diameter in strawberry fruit inoculated with Botrytis cinerea. Terpinen-4-ol treatment enhanced the expression of genes involved in JA synthesis (FaLOX, FaAOC, and FaOPR3) and signaling (FaCOI1), as well as genes related to disease defense (FaPAL, FaCHI, and FaGLU). In contrast, treatment with the JA biosynthesis inhibitor salicylhydroxamic acid (SHAM) accelerated disease development and inhibited the induction of gene expressions by terpinen-4-ol. We conclude that the JA pathway participates in the induction of disease resistance by terpinen-4-ol in strawberry fruit. More generally, the results illuminate the mechanisms by which disease resistance is enhanced by essential oils.

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Terpinen-4-ol treatment maintains quality of strawberry fruit during storage by regulating sucrose-induced anthocyanin accumulation

Cited by 20 publications

References 41 publications

Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione

Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione

Two MYB and Three bHLH Family Genes Participate in Anthocyanin Accumulation in the Flesh of Peach Fruit Treated with Glucose, Sucrose, Sorbitol, and Fructose In Vitro

The Jasmonic Acid Signaling Pathway is Associated with Terpinen-4-ol-Induced Disease Resistance against Botrytis cinerea in Strawberry Fruit

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