Terrilactibacillus laevilacticus gen. nov., sp. nov., isolated from soil

Prasirtsak, Budsabathip; Thongchul, Nuttha; Tolieng, Vasana; Tanasupawat, Somboon

doi:10.1099/ijsem.0.000954

Cited by 17 publications

(6 citation statements)

References 34 publications

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“…MK-7 was the major menaquinone (100 %), which was in accordance with T. laevilacticus NK26-11 T [1]. Strain BCM23-1 T contained major fatty acids (>10 % of the total fatty acids) C 18 : 1 ω9 c (25.6 %), anteiso-C 17 : 0 (23.6 %) and anteiso-C 15 : 0 (22.6 %) when cultivated in MRS broth and anteiso-C 17 : 0 (48.7 %), anteiso-C 15 : 0 (24.5 %) and iso-C 16 : 0 (13.2 %) when cultivated on GYP agar plate.…”

Section: Full-textsupporting

confidence: 53%

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Terrilactibacillus tamarindi sp. nov., isolated from bark of Tamarindus indica

Kingkaew

Nuhwa

Piluk

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod, designated strain BCM23-1T, was isolated from bark of Tamarindus indica collected from Chiang Mai Province, Thailand. This strain produced d-lactic acid from glucose. It grew at 20–45 °C (optimum, 30 °C), pH 3.5–9 (optimum, pH 7.0) and in the presence of 1–4 % (w/v) NaCl. The cell-wall peptidoglycan contained meso-diaminopimelic acid (A1γ). The major isoprenoid quinone was menaquinone 7 (MK-7). Polar lipids analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid. The predominant cellular fatty acids were anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0 when cultivated on GYP agar plates. The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T was 98.3 %. The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%. The values of ANIb, ANIm and digital DNA–DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively. The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp. nov. is proposed. The type strain is BCM23-1T (=LMG 31662T=JCM 33748T=TISTR 2841T).

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confidence: 53%

“…The genus Terrilactibacillus was firstly proposed by Prasirtsak et al . [1]. The type species of the genus is Terrilactibacillus laevilacticus .…”

Section: Full-textmentioning

confidence: 99%

Terrilactibacillus tamarindi sp. nov., isolated from bark of Tamarindus indica

Kingkaew

Nuhwa

Piluk

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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“…torquens, and L. delbrueckii subsp. lactis QU41 and some metabolically engineered strains such as Lactobacillus plantarum, Escherichia coli, Corynebacterium glutamicum, Enterobacter asburiae, and Saccharomyces cerevisiae including a novel genus Terrilactibacillus used in this study have been reported for the production of optical pure D-lactic acid (Tashiro et al 2011;Mimitsuka et al 2012;Li et al 2013;Zhao et al 2014;Cingadi et al 2015;Bai et al 2016;Prasirtsak et al 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Terrilactibacillus genus is comprised of the catalasepositive bacteria that can be grown in the presence of oxygen (Prasirtsak et al 2016). It produces the higher cell biomass and lactic acid than other previously reported D-lactate producers that usually lack catalase, and thus, required growth and lactic acid production under anaerobic conditions (Prasirtsak et al 2017).…”

Section: Introductionmentioning

confidence: 99%

D-Lactic acid fermentation performance and the enzyme activity of a novel bacterium Terrilactibacillus laevilacticus SK5–6

et al. 2019

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The aim of this study was to prove that Terrilactibacillus laevilacticus SK5-6, a novel D-lactate producer, exhibited a good fermentation performance comparing to the reference D-lactate producer Sporolactobacillus sp. Methods Glucose bioconversion for D-lactate production and the activity of five key enzymes including phosphofructokinase (PFK), pyruvate kinase (PYK), D-lactate dehydrogenase (D-LDH), L-lactate dehydrogenase (L-LDH), and lactate isomerase (LI) were investigated in the cultivation of T. laevilacticus SK5-6 and S. laevolacticus 0361 T. Results T. laevilacticus SK5-6 produced D-lactate at higher yield, productivity, and optical purity compared with S. laevolacticus 0361 T. T. laevilacticus SK5-6, the catalase-positive isolate, simultaneously grew and produced D-lactate without lag phase while delayed growth and D-lactate production were observed in the culture of S. laevolacticus 0361 T. The higher production of Dlactate in T. laevilacticus SK5-6 was due to the higher growth rate and the higher specific activities of the key enzymes observed at the early stage of the fermentation. The low isomerization activity was responsible for the high optical purity of D-lactate in the cultivation of T. laevilacticus SK5-6. Conclusion The lowest specific activity of PFK following by PYK and D/L-LDHs, respectively, indicated that the conversion of fructose-6-phosphate was the rate limiting step. Under the well-optimized conditions, the activation of D/L-LDHs by fructose-1,6-phosphate and ATP regeneration by PYK drove glucose bioconversion toward D-lactate. The optical purity of D-lactate was controlled by D/L-LDHs and the activation of isomerases. High D-LDH with limited isomerase activity was preferable during the fermentation as it assured the high optical purity.

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“…In 2016, Prasirtsak et al reported that Terrilactibacillus laevilacticus SK5-6, a novel D-lactic acid producing bacterium, was isolated from soil in Thailand (Prasirtsak et al, 2016). This isolate was preliminarily identified as the potent industrial strain.…”

Section: The Novel D-lactic Acid Producing Bacteriummentioning

confidence: 99%

Optimization of d-lactic acid production by terrilactibacillus laevilacticus SK5-6 and fermentation process scale up in 30 litre-fermentor

Toliang

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PLA, which is one of biodegradable plastic stereocomplex is made by block polymerization of optically pure L- and D-lactic acid. With stereoblock structure, heat and mechanical properties of PLA products can be improved. Thus, the demand of D-lactic acid has been increased. However, the market supply is still limited.?In-house work reported that Terrilactibacillus laevilacticus SK5-6, the novel bacterium has been isolated from soil and later identified as the potent D-lactic acid producer. Moreover, it could produce high D-lactic acid?from glucose with high optical purity?in flask scale. To improve the fermentation performance, further process optimization was conducted in a 5 L stirred fermentor. The effects of inoculum size, seed?age and volume of seed on D-lactic acid production were determined for establishing the platform. This work shows that the novel strain could produce high concentration of D-lactic acid with high yield and optical purity as well as the flask scale production. It was observed that 88.0 g/L of D-lactic acid?with yield of 0.87 g/g glucose and productivity of 1.83 g/L/h were obtained with the mid log phase of 1% inoculum size and 10% seed transfer. Furthermore, the high optical purity of 99.3% could also be obtained from this isolate in the 5 L fermentor operation with above conditions. To develop the fermentative process scale up platform for D-lactic acid in the 30 L stirred fermentor. The seed train was developed for fermentation by used the conditions that had optimized in 5 L fermentor. In addition, the engineering parameters were tested as scale up factors by assuming constant mass transfer pattern in both scales. The results of testing scale up in 30 L fermentor show that T. laevilacticus SK5-6 could produce 81.5 g/L of D-lactic acid and productivity of 2.09 g/L/h, which was higher than 5 L scale slightly. They were obtained with 10% preculture seed transfer (seed in 250 mL flask) and using power input per volume (P/V) for agitation criteria. In addition, the high optical purity of 99.3% could also be obtained in the 30 L fermentor as well as production in 5 L scale. Therefore, the novel D-lactic acid producer, T. laevilacticus SK5-6 should be suggested as the potential strain for D-lactic acid production in the industrial scale.??

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Terrilactibacillus laevilacticus gen. nov., sp. nov., isolated from soil

Cited by 17 publications

References 34 publications

Terrilactibacillus tamarindi sp. nov., isolated from bark of Tamarindus indica

Terrilactibacillus tamarindi sp. nov., isolated from bark of Tamarindus indica

D-Lactic acid fermentation performance and the enzyme activity of a novel bacterium Terrilactibacillus laevilacticus SK5–6

Optimization of d-lactic acid production by terrilactibacillus laevilacticus SK5-6 and fermentation process scale up in 30 litre-fermentor

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