To ensure the reliability and accuracy of tests (measurements),the testing laboratory must calculate the measurement uncertainty for eachtest method and reflect this characteristic in its test (measurement) reports.Calculation of test uncertainty is carried out using various methods. In thisarticle, the uncertainty calculation was carried out using the least squaresmethod, when the desired value of the output parameter is determined fromthe graph. For this purpose, a series of tests was carried out: the toxicity ofcombustion products was determined from the lethality of white laboratorymice and a graph of this dependence was plotted. The graph determines thetoxicity of combustion products at 50% lethality of white laboratory mice.Using the least squares method, confidence limits for the estimate of themeasured value were calculated and graphically shown in this graph. As aresult of calculations, the uncertainty of the toxicity of combustionproducts is about 25%; such a large scatter of data using this methodconsists of the error of the measuring equipment and the calibration of thisequipment, the test sample and its composition, as well as thecharacteristics of white laboratory mice and their behavior. These resultsfor calculating test uncertainty can be used for testing laboratories thatconduct fire safety tests of materials.