Test‐1 analyzer and conventional Westergren method for erythrocyte sedimentation rate: A comparative study between two laboratories

Sönmez, Çiğdem; Doğan, Özlem; Kaymak, Ayşegül Öztürk; Akkaya, Nedim; Akın, Kadir Okhan; Guntaş, Gülcan

doi:10.1002/jcla.22384

Cited by 13 publications

(16 citation statements)

References 18 publications

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“…Sonmez et al also found a poor correlation between the two methods at high ESR values ( p > 0.10). 9 Dhruva et al also had similar observations in patients with HCT between 30 to 35%. They realized that samples with high ESR values vary considerably around the mean difference compared with samples which had normal ESR readings.…”

Section: Discussionmentioning

confidence: 60%

“…Sonmez et al and Romero et al, similar to our study, evaluated the effect of low HCT (< 35%) on ESR levels. Sonmez et al 9 used samples of 755 patients and divided the corrected ESR values into low (≤20 mm/hour) and high (> 20 mm/hour) range and according to HCT (≥35%, < 35%) levels. Like our study, Sonmez et al 9 also found that in anemic patients, ESR values measured by the direct Westergren method were higher than automated method.…”

Section: Discussionmentioning

confidence: 99%

“…Sonmez et al 9 used samples of 755 patients and divided the corrected ESR values into low (≤20 mm/hour) and high (> 20 mm/hour) range and according to HCT (≥35%, < 35%) levels. Like our study, Sonmez et al 9 also found that in anemic patients, ESR values measured by the direct Westergren method were higher than automated method. Hence, Fabry’s formula was applied to correct the overestimation.…”

Section: Discussionmentioning

confidence: 99%

“…Since ESR performed by the manual standard Westergren method is also affected by hematocrit, Fabry’s formula (Westergren ESR X 15/55-HCT) can be used to correct ESR values obtained by the manual method. 9 Also, to overcome the confounding factors, recently many new automated techniques for measuring ESR have been developed and introduced in clinical laboratories. They also provide many advantages like safety of operators, reducing biohazards risks, quicker results, speedy processing time, and ease of performance of other hematological tests (erythrocyte, leukocyte and reticulocyte concentrations) in a single specimen.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Analysis of Erythrocyte Sedimentation Rate Measured by Automated and Manual Methods in Anaemic Patients

et al. 2020

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Purpose Erythrocyte sedimentation rate (ESR) is a widely used indicator of inflammation and a routinely done hematology investigation to monitor patients of autoimmune and infectious diseases. We aimed to compare the ESR results obtained by Roller 20LC automated instrument and standard reference Westergren method and analyzed the effect of anemia (hematocrit) on ESR measurements through the automated method. Methods We analyzed 1377 random anemic OPD patients (hematocrit [HCT] < 35%) for ESR levels measured by Roller 20LC using EDTA blood and Westergren method using citrated blood for a one and half year period from January 1, 2018 to June 30, 2019. Fabry’s formula was used to correct the Westergren ESR. Results The total number of samples after evaluation were divided into low (n = 232), intermediate (n = 417), high (n = 406), and very high range of ESR (≥100 mm/hr; n = 422). Mean difference between values of corrected and automated ESR for the low, intermediate, high and very high ESR range was 2.33 ± 5.03, 10.95 ± 8.04, 28.22 ± 19.11 and 43.3 ± 19.22 mm/hr, respectively. The 95% limit of agreement calculated by the Bland–Altmann analysis between the two methods for low-ESR range was −7.53 to 12.2 (highest correlation coefficient –0.65), while for very high ESR, range was −5.1 to 81.5 (least coefficient of 0.18) (p < 0.001). Conclusion In laboratories with high-sample load and where manual measurement may be tedious, the automated method of ESR measurement can safely replace the Westergren method for low-ESR values in patients with low hematocrit. While for high-ESR values, validation by the standard Westergren method may be needed.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Analysis of Erythrocyte Sedimentation Rate Measured by Automated and Manual Methods in Anaemic Patients

et al. 2020

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“…The control blood sample ESR was 6 mm/h and CQDs‐treated blood sample was 7.5 mm/h. The normal range of ESR by Westergren method is 15 mm/h . The obtained ESR value in CQDs‐treated (test) blood sample was within the acceptable range.…”

Section: Resultsmentioning

confidence: 57%

Sweet Corn (Zea mays L. var. rugosa) Derived Fluorescent Carbon Quantum Dots for Selective Detection of Hydrogen Sulfide and Bioimaging Applications

Rajendran

Kasthuri³

et al. 2019

ChemistrySelect

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We report a green, simple, and facile fluorometric method for the detection of hydrogen sulfide (S 2À ) using bluish green carbon quantum dots (CQDs) derived from sweet corn (Zea mays L. var. rugosa) as carbon source. The optical properties of the CQDs were studied using UV-visible, PL spectra and lifetime measurements. High-resolution transmission electron microscopy (HR-TEM), atomic force microscope (AFM) and dynamic light scattering (DLS) were used for morphological studies. Xray diffraction (XRD) technique was used for phase analysis. Zeta potential, Fourier transform infrared (FT-IR), Raman and Xray photoelectron spectroscopy (XPS) were used to measure the charge and composition of QDs. The prepared CQDs having size less than 2.4 nm with bluish green emission at ∼ 470 nm and showed excellent stability for more than 6 months in aqueous medium. The synthesized CQDs showed highly sensitive and selective detection of sulfide ions (S 2À ) in aqueous medium over other toxic metal ions and inorganic salts, and the detection limit was 8 nM in the linear range from 5 to 100 nM. The synthesized CQDs had excellent hemocompatibility as evidenced from the hemolytic assay (2.86 %) and ESR estimation (7.5 mm/h) on human RBCs. The cell viability of CQDs was evaluated on Vero and A549 cell line by MTT assay, which produced more than 90 % of cell viability in both cell lines at 250 μg/mL concentration. Owing to their biocompatible nature, the synthesized CQDs were used as a fluorescent probe for in vitro bioimaging applications.

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Determination of the erythrocyte sedimentation rate using the hematocrit‐corrected aggregation index and mean corpuscular volume

Higuchi

Watanabe

2023

Clinical Laboratory Analysis

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Background Determination of the erythrocyte sedimentation rate (ESR) by measurement of erythrocyte aggregation is an alternative to the Westergren method and can be performed rapidly. However, its principle is opaque and the ESR values obtained can deviate from Westergren method values (WG ESR) due to hematocrit. Furthermore, WG ESR is affected by particle size, but no studies have examined the effect of individual mean corpuscular volumes (MCVs). Methods Simultaneous measurement of the erythrocyte aggregation index (AI) over a 5‐s interval and determination of the complete blood count in 80 μL blood from 203 patients were performed (hematocrit, 21.4%–52.3%; MCV, 62.7–114.1 fL). ESR values were calculated with the hematocrit‐corrected AI (HAI) for comparison with WG ESR. We improved the calculation formula by using MCV. Results The sedimentation velocity of a single erythrocyte in the samples agreed well with an exponential function of HAI. ESR values calculated using HAI showed excellent correlation with WG ESR ( r = 0.899, p < 0.001; Bland–Altman analysis: bias 2.76, limits of agreement (LOA) −24.5 to 30.0), but the difference between the calculated ESR and WG ESR increased with decreasing MCV. Calculation of ESR considering both HAI and MCV eliminated the MCV‐dependent deviation and improved the correlation with WG ESR ( r = 0.920, p < 0.001, bias −2.17, LOA −24.6 to 20.3). Conclusion Calculation using HAI and MCV can rapidly provide ESR values that are highly correlated with WG ESR in clinical specimens over a wide range of hematocrit and MCV values.

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Test‐1 analyzer and conventional Westergren method for erythrocyte sedimentation rate: A comparative study between two laboratories

Abstract: The study showed that the role of the hospital and the capacity of testing are important in choosing the instrument for measuring ESR. Furthermore, the patient profile, especially malignancy possibility and Hct level, may be important for instrument selection.

Cited by 13 publications

References 18 publications

Comparative Analysis of Erythrocyte Sedimentation Rate Measured by Automated and Manual Methods in Anaemic Patients

Comparative Analysis of Erythrocyte Sedimentation Rate Measured by Automated and Manual Methods in Anaemic Patients

Sweet Corn (Zea mays L. var. rugosa) Derived Fluorescent Carbon Quantum Dots for Selective Detection of Hydrogen Sulfide and Bioimaging Applications

Determination of the erythrocyte sedimentation rate using the hematocrit‐corrected aggregation index and mean corpuscular volume

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